Octet® BLI Label-Free Detection Systems for Biomolecular Interactions Analysis

Unprecedented Time and Cost Savings For Kinetics, Affinity, and Concentration Assays

Based on Bio-Layer Interferometry (BLI), Octet® BLI systems enable real-time, label-free analysis for the determination of kinetics, affinity and antibody/protein quantitation. This fluidics-free instrument platform offers several advantages over other label-free techniques like Surface Plasmon Resonance (SPR). The Octet® BLI platform also provides robust monitoring of biomolecular interactions to overcome limitations in throughput, performance and cost associated with traditional technologies such as enzyme-linked immunosorbent assay (ELISA) or high-performance liquid chromatography (HPLC). These fluidics-free, low-maintenance, sensitive systems increase lab productivity, reduce costs and shorten experimental timelines.  
The Sartorius Octet® BLI platform has become an invaluable technique analyzing therapeutic biomolecules at various stages of development. With unparalleled ease-of-use, the instruments transform workflows beyond the limitations of label-free methods, providing comprehensive characterization capabilities across a broad range of applications critical for efficient biotherapeutics development and production. 
With a wide range of ligand capture surfaces that allow for immobilization of antibodies, recombinant proteins, DNA, RNA and virus-like particles, the Octet® BLI platform has become a must-have laboratory tool for biomolecular interactions studies.  
Benefits to accelerate your research:

  • Higher Throughput - Protein quantitation in as little as two minutes, and parallel processing of up to 96 samples 
  • Fast Time to Results – Automated analysis for faster time-to-market and faster publications
  • Real-time Analysis – Binding interactions are continuously monitored as they happen. Don’t miss out on subtle kinetic behavior patterns.
  • Crude Sample Compatibility - Analytes can be measured in crude, unpurified mixtures such as cell lysates or hybridoma supernatants.
  • Ease of Use – Simple, integrated user interface designed for biologists. Spend less time learning instrumentation and more time deriving insights.
  • Low Total Cost of Ownership – Low-maintenance system has no fluidics modules to clog, so no downtime while waiting for replacements.

Discover Biolayer Interferometry (BLI) Technology

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Octet® R Series

Accelerate Your Research with an Upgradeable, Label-Free Protein Analysis Platform

The Octet® BLI is an easy-to use, real-time analytical tool that rapidly screens critical quality attributes.

2-Channel System: Octet® R2

Grows With Your Biomolecule Characterization Needs

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4-Channel System: Octet® R4

The Perfect Balance Between Cost and Throughput with Given Leeway

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Octet R8 instrument

8-Channel System: Octet® R8

Unmatched Flexibility and Versatility in Protein Analysis

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Octet® RH16 and RH96

Unprecedented Throughput for Industry Applications

Octet® BLI rapidly determines recombinant protein concentration as a control parameter in cell line development.

96-Channel Ultra High Throughput Octet System: Octet® RH96

Monitors up to 96 biosensors simultaneously, enabling label-free detection for protein quantitation and kinetic characterization at unmatched speed

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16-Channel High Throughput Octet System: Octet® RH16

Ideal for high-throughput applications that demand high sensitivity and low sample volume requirements

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Octet®️ N1 – Label-Free Assays in a Drop

Octet® N1

Label-free assays in a drop

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Benefit for Label-Free Detection Requirements

Lab technician with Octet RH96
Experimental Simplicity to De-Risk Your Journey and Simplify Progress

A microfluidic-free design allows for unprecedented time and cost savings for binding kinetics, affinity and analyte concentration measurement with high precision, accuracy and a broad dynamic range.

Analyze Tough Samples Faster 

Parallel processing of up to 96 samples - coupled with minimal sample preparation - provides unprecedented throughput. Use fluidics-free assays with 96- and/or 384-well sample plates and ready-to-use biosensors for flexibility in sample types from large biologics to small molecules, purified or in crude form.

Perfect for Multi-User Environments 

Octet® BLI systems are known for reliable, user-friendly, trouble-free operation and have quickly become the go-to systems in academic and industrial labs for their ease-of-use, robustness and application flexibility.

Transfer Methods to QC and Manufacturing with Confidence

Rapidly develop and validate Octet® BLI assays for lot release, ligand binding, quantitation and impurity assays.

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Featured BLI System Resources

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Case Study

Bispecific Antibodies: From Research to Clinical Application

Learn how Octet BLI transforms our approach to drug discovery and development

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Octet R Series System brochure cover

Octet® R Series Systems

Accelerate your research with an upgradeable, label-free protein analysis platform

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Biomolecular Binding Kinetics Assays on the Octet® Platform thumbnail
Application Note

Biomolecular Binding Kinetics Assays on the Octet® Platform

Introducing label-free platforms, the technology, capabilities, and advantages of label-free binding assessment

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Comparability Studies: Minimize Time, Maintain Quality

Developing Methods for Comparability Studies of Therapeutic Monoclonal Antibodies

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Instant Access

Octet top 10 reasons to choose BLI

Top Ten Reasons to Choose Sartorius Octet®️ BLI Technology to Accelerate Your Research, Development, and Manufacturing

PDF | 167.1 KB
Octet® Systems in Bioprocessing: Easy-to-Use and Cost-Effective Tools for Multiple Applications
Application Guide

Octet® Systems in Bioprocessing: Easy-to-Use and Cost-Effective Tools for Multiple Applications

PDF | 282.5 KB

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