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Bio-Layer Interferometry is an analytical technique that monitors the interference pattern of white light reflected from two surfaces; a layer of immobilized protein on the biosensor tip and an internal reference layer.
The binding of an analyte in solution to the immobilized protein (ligand) on the biosensor results in an increase in optical thickness which in turn causes a shift in the interference pattern resulting in a wavelength shift. This shift in wavelengths (nm) is reported in real time. Only molecules that associate or dissociate and cause a change in optical thickness elicit a response pushing the boundaries of label-free biomolecular interaction analysis (BIA) in terms of scalable throughput, flexibility, and sample compatibility.