Optimal Combinations of CellGenix^® Reagents Boost Natural Killer Cell Expansion, Viability, and Purity

Abstract 

Cell and gene therapies, including immunotherapy with natural killer (NK) cells, have emerged as promising avenues for treating various diseases. However, successfully producing these therapies requires manufacturers to overcome operational challenges associated with yield and long-term cell culture. The selection of an optimal cell culture media and cytokine cocktail is an important step in generating high yields of functional cytotoxic NK cells.

This application note presents the performance of CellGenix^® GMP Stem Cell Growth Medium (SCGM) and CellGenix^® recombinant human cytokines for the expansion and cultivation of NK cells. Fold expansion, viability, phenotype, and cytotoxicity of NK cells cultured in CellGenix^® GMP SCGM were compared to those cultured in other commercially available NK cell media. The results suggest that the chosen medium and cytokine cocktails deliver the highest expansion while maintaining cytotoxicity and purity.