Induced Pluripotent Stem Cells (iPSCs) and Embryonic Stem Cells (ESCs)
In 2012, the Nobel prize for physiology or medicine was awarded for the discovery that adult somatic cells can be reverted to a pluripotent stem cell state using reprogramming factors. Since then, induced pluripotent stem cells (iPSCs) have become one of the most promising pathways for therapeutic applications.
Pluripotent stem cells, including iPSCs and embryonic stem cells (ESCs), have the innate ability to self-renew indefinitely and differentiate into any cell type. This confers a unique advantage when compared to other cell therapies since the starting cells can be differentiated into any specific cell type required for the final therapy. Pluripotent stem cells can also serve as a canvas for gene modification before they are differentiated. This enhances the targeted therapeutic benefit by yielding not only the desired phenotypic characteristics but also the genetic characteristics to maximize efficacy. Additionally, the effects of graft versus host disease (GvHD) can be mitigated, with certain genetic modifications. Finally, ESCs and iPSCs can be used as new cell sources for the generation of DC, T cell therapies, NK cells and pancreatic islets.
To ensure a seamless transition from preclinical development to commercial manufacturing, we offer CellGenix® cytokines and growth factors both in GMP and preclinical grade.
Our GMP cytokines are provided with documented evidence of purity, potency, consistency and stability. In combination with our expert regulatory and technical support this will help simplify your raw material qualification and validation efforts.
CellGenix® GMP recombinant cytokines are produced following all applicable GMP guidelines and allow for the safe use in accordance with USP Chapter <1043>, Ph. Eur. General Chapter 5.2.12 and ISO 20399:2022.
Test Growth Factor and Cytokines Samples for iPSC| ESC Cells
Samples for cell and gene therapy as well as regenerative medicine.
iPSC and ESC Growth Factors and Cytokines | ||
|---|---|---|
| FGF-2 | More Info | Visit eShop |
TGF-β1 | ||
Activin A | More Info | Visit eShop |
| EGF | More Info | Visit eShop |
IL-1β | More Info | Visit eShop |
OSM | ||
SCF | ||
TPO | ||
FGF-2
CellGenix® Recombinant Human FGF‑2 (rh FGF‑2, also known as bFGF) is an animal component–free growth factor that enhances proliferation of mesenchymal stem cells (MSCs), supports chondrocyte proliferation and differentiation, and maintains undifferentiated pluripotent stem cells (PSCs). Both preclinical and GMP grades are produced in a dedicated GMP facility.
FGF‑2 plays a key role in wound healing and tumor development by promoting angiogenesis and is used in cell and gene therapy for expansion of bone marrow– and adipose-derived MSCs.
TGF-β1
CellGenix® Recombinant Human TGF‑β1 (rh TGF‑β1) is an animal component–free cytokine that supports expansion of iPSCs, ESCs, MSCs, and regulatory T cells (Tregs) and drives T cell differentiation into Tregs. Both preclinical and GMP grades are produced in a dedicated GMP facility.
TGF‑β1, a member of the TGF‑β superfamily, regulates cell differentiation, proliferation, and apoptosis and is secreted by many immune cells, including T cells, B cells, macrophages, and monocytes. In cell and gene therapy, it is used to expand iPSCs, ESCs, MSCs, and Tregs.
CellGenix® rh TGF‑β1 is produced in a human amniocyte cell line (CAP®), enabling authentic human post‑translational modifications that cannot be achieved with bacterial expression systems.
Activin A
CellGenix® Recombinant Human Activin A is an animal component–free growth factor that promotes definitive endoderm differentiation of ESCs and iPSCs and helps maintain their undifferentiated state. Both preclinical and GMP grades are produced in a dedicated animal-free GMP facility.
Activin A, a member of the TGF‑β superfamily, regulates cell proliferation, differentiation, apoptosis, and wound repair. In cell and gene therapy, it is used to drive ESC/iPSC differentiation into definitive endoderm and to support maintenance and self‑renewal of pluripotent stem cells.
EGF
CellGenix® Recombinant Human EGF (rh EGF) is an animal component–free growth factor that promotes expansion of mesenchymal stem cells (MSCs) and supports differentiation of ESC‑ and iPSC‑derived hepatoblasts. Both preclinical and GMP grades are produced in a dedicated animal-free GMP facility.
EGF drives cell growth, proliferation, and differentiation and is present in various body fluids. In cell and gene therapy, it is used to expand bone marrow– and adipose-derived MSCs and generate stem cell–derived hepatocytes.
IL-1β
CellGenix® Recombinant Human IL‑1β (rh IL‑1β) is an animal component–free cytokine that stimulates maturation of immature dendritic cells (DCs) and supports generation of megakaryocytes from pluripotent stem cells (PSCs). Both preclinical and GMP grades are produced in a dedicated animal-free GMP facility.
IL‑1β is a proinflammatory cytokine mainly produced by monocytes and activated macrophages. It drives inflammatory responses in T cells, NK cells, and B cells by inducing other proinflammatory cytokines and interferons and modulating DC cytokine secretion. In cell and gene therapy, CellGenix® IL‑1β is used for ex vivo maturation of DCs.
OSM
CellGenix® Recombinant Human Oncostatin M (rh OSM) is an animal component–free cytokine that drives differentiation of ESC‑ and iPSC‑derived hepatoblasts into hepatocytes. It is produced in a dedicated animal-free GMP facility for safe use in ATMP manufacturing.
OSM, member of the interleukin-6 (IL-6) family of cytokines closely related to LIF, is mainly produced by T cells and monocytes and is involved in liver development, hematopoiesis, inflammation, and bone remodeling.
SCF
CellGenix® Recombinant Human Stem Cell Factor (rh SCF) is an animal component–free cytokine that supports survival and differentiation of hematopoietic stem and progenitor cells, drives cord blood–derived CD34⁺ cells toward NK cells, and is used to differentiate ESCs and iPSCs into megakaryocytes and T cells. Both preclinical and GMP grades are produced in a dedicated animal-free GMP facility.
SCF, produced by fibroblasts and endothelial cells, is essential for hematopoiesis, spermatogenesis, and melanogenesis and is used in cell and gene therapy of HSCs used for transplantation.
TPO
CellGenix® Recombinant Human Thrombopoietin (rh TPO) is an animal component–free cytokine that supports self‑renewal and maintenance of hematopoietic stem cells (HSCs), drives their differentiation into NK cells, and promotes ESC/iPSC differentiation into megakaryocytes. Both preclinical and GMP grades are produced in a dedicated animal-free GMP facility.
TPO is a liver- and kidney-derived glycoprotein hormone that regulates platelet production by stimulating megakaryocyte formation. In cell and gene therapy, it is used for ex vivo expansion and differentiation of HSCs.
