Octet® BLI Resource Library

Research the Industry Standard for Label-Free of Biomolecular Interactions Analysis (BIA)

Research the Industry Standard for Label-Free of Biomolecular Interactions Analysis (BIA) - The Octet® BLI platform enables label-free biomolecular interaction analysis based on Bio-Layer Interferometry (BLI) technology. Download the latest application notes, infographics, eBooks, and more to support your label-free research and development.

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Featured Octet® Resources

Octet biosensors
Tutorial

Octet® Software Tutorials

Mastering Octet® BLI Discovery and Analysis Studio Software for Streamlined Biomolecular Interaction Analysis

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A compendium for successful BLI and SPR assays
Application Guide

A Compendium for Successful BLI and SPR Assays

A comprehensive guide for designing and performing label-free biomolecular interaction analysis.

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White Paper

The End of End-Point Assays

Faster time-to results allows assessment of accurate and precise data earlier in the workflow

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Application Note

Advances in Immunogenicity Evaluation

Paving the Way for Safer Biologic Therapies

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Application Note

Biomolecular Binding Kinetics Assays on the Octet® Platform

Explore advantages of label-free binding assessment in therapeutics development and life sciences research.

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Application Guides (18)

Structure Function Relationship: Effect of Changes in Higher Order Structure to Analyte Ligand Binding
Application Guide

Structure Function Relationship

Effect of Changes in Higher Order Structure to Analyte Ligand Binding

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Application Guide

Harmonizing Regulatory Guidelines for Assay Validation

Current regulatory guidelines for assay validation were harmonized for kinetic-based assay to derive a common set of parameters.

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Application Guide

Label-free to Decipher CRISPR/Cas Mechanisms

Octet® BLI to measure and characterize CRISPR/Cas mediated binding and Gene activation

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A compendium for successful BLI and SPR assays
Application Guide

A Compendium for Successful BLI and SPR Assays

A comprehensive guide for designing and performing label-free biomolecular interaction analysis.

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Octet® BLI Workflows in Small-Molecule Interaction Discovery and Characterization
Application Guide

Small-Molecule Interaction Discovery and Characterization

With Octet® BLI Workflows

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Cell Line Development: Accelerating Antibody Discovery by Monitoring Titer and Glycosylation With the Octet® Platform
Application Guide

Cell Line Development

Accelerating Antibody Discovery by Monitoring Titer and Glycosylation With the Octet® Platform

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Application Guide

Determining Nanoparticle Vaccine Antigenic Efficiency

Nanoparticle (NP) vaccine and antigen delivery platforms have emerged as a promising approach due to their ability to interact with immune components

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Application Guide

Stability, Formulations and Aggregation Studies

Expanding Octet® Applications in Downstream Biologics Characterization

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Application Guide

Accurate Measurements on the Octet® Platform

Without Added Sample Purification Steps

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Application Guide

Advancing Development of Coronavirus Vaccine and Therapeutics

BLI Technology Plays a Vital Role in the Race for a SARS-CoV-2 Treatment

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Application Guide

Recent Insights into Covid-19 Binding Epitopes

Researchers are using the Octet® system to understand the virus's binding mechanisms as a first step in the development of a vaccine

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Octet® Systems: Modernize Biopharmaceutical QC Testing to Increase Efficiency
Application Guide

Modernize Biopharmaceutical Quality Control Testing

Quality control of biological products to support clinical trials and post market assay activities require the evaluation of multiple critical product...

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Application Guide

Label-Free Technologies

For Accurate Determination of Kinetics and Affinity Rate Constants

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Application Guide

Ligand Binding Assays That Meet Compliance with Octet® BLI Systems

Octet® GxP packages support the GMP compliant implementation, including 21 CFR Part 11 software, validation packages, and biosensor validation support...

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Application Guide

Characterizing Membrane Protein Interactions

By Octet® Bio-Layer Interferometry (BLI) Platforms

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Application Guide

A Simple Method to Determine Relative Potency (EC50)

Using Octet® BLI Technology

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Application Guide

Simplifying Biotherapeutic Manufacturing and Quality Control

With Label-Free Biosensor Technology

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Octet® Systems in Bioprocessing: Easy-to-Use and Cost-Effective Tools for Multiple Applications
Application Guide

Octet® Systems in Bioprocessing: Easy-to-Use and Cost-Effective T...

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Application Notes (29)

Application Note

An Essential Guide to Fcγ Receptor Binding

A guide to Fcγ receptors binding and assay design. Effectively study binding kinetics and affinities of IgG1, IgG2, & IgG4 antibodies with Fcγ rec...

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Application Note

Advances in Immunogenicity Evaluation

Paving the Way for Safer Biologic Therapies

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Optimizing Kinetics Assays to Avoid Avidity Effects
Application Note

Optimizing Kinetics Assays to Avoid Avidity Effects

A fundamental understanding of the differences between affinity and avidity is essential when designing and developing assays

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Application Note

Rapid, Automated, At-Line Adenovirus Quantitation Using Octet® BL...

Learn how we report the development of a rapid, high-throughput capsid assay for AV quantification on the Octet® BLI platform that demonstrates excell...

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Octet R4 SARS-CoV-2 application note
Application Note

Octet® R4 Bio-Layer Interferometry Platform SARS-CoV-2

Biophysical Characterization of the Binding of SARS-CoV-2 to the ACE2 Protein Using the Octet® R4 Bio-Layer Interferometry Platform

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Application Note

Quantitation of Fc-Fusion Protein in Cell Culture Supernatants

The cell line development group at Biogen IDEC needed a robust assay for the measurement of Fc-fusion protein in crude cell culture supernatants.

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Octet® AAVX Biosensors for Rapid and Direct Quantitation of AAV Capsids
Application Note

Measure AAV Titer in as Little as 15 Minutes

Rapid Quantitation of AAV Capsids for Various Serotypes for Bioprocess Development and Quality Control in Gene Therapy

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Application Note

Measure Pharmacokinetics of Monoclonal Antibodies

Scientists from Elanco® use Octet® BLI to rapidly design and establish a preclinical PK method for analyzing canine antibodies

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Application Note

Customized Quantitation of Recombinant Therapeutic Proteins

Using Octet® SAX Biosensors

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Analysis of FcRn-Antibody Interactions on the Octet® Platform
Application Note

Analysis of FcRn-Antibody Interactions on the Octet® Platform

Assay design recommendations and best practices for producing the highest quality of kinetic data.

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Application Note

High-Throughput Assays for Epitope Binning and Cross-Competition Analy...

Epitope binning through cross competition assays is a powerful tool in evaluating functional diversity in antibody clones.

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Analysis of Fc-Gamma Receptor-IgG Interactions on the Octet® Platform
Application Note

Analysis of Fc Gamma Receptor IgG Interactions on Octet® Platform

Whether you're assessing FcRn-Antibody or FcγR-IgG binding interactions, you need a high throughput, versatile, and easy to use solution.

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Application Note

Second Generation Anti-Human IgG Fc Capture Biosensors

For Affinity Characterization of Human IgG-Antigen Interactions and Quantitation of Human IgGs or Human Fc Region Containing Proteins 

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Application Note

Mannose Glycans Content Screening for Human-mAb Samples

A novel approach for relative mannose content screening and ranking of human monoclonal antibody (mAb) samples using the Octet® GlyM Kit

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Application Note

Biomolecular Binding Kinetics Assays on the Octet® Platform

Explore advantages of label-free binding assessment in therapeutics development and life sciences research.

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Validated Quantitation and Activity Assay of Antibody Fragment Molecule (Fab) for Process Development and Quality Control
Application Note

Validated Quantitation and Activity Assay of FaB

For Process Development and Quality Control

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Application Note

MAb Quantitation Protein A HPLC vs Protein A Bio-Layer Interferometry

Rapid, accurate and cost-effective quantitation of monoclonal antibodies (MAbs) is essential for bioprocessing.

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Application Note

Potency Assay Development Qualification and Validation Strategies

A method for evaluating the binding of an Fc gamma receptor III (FcγR) molecule to the widely characterized NIST mAb.

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A Rapid Method to Quantitatively Screen Bispecific Antibodies Using Protein A and Octet® His1K Biosensors
Application Note

Screen Bispecific Antibody Using Protein A and His1K Biosensors

Rapid quantitative screening of bispecific antibodies using Protein A and HIS1K biosensors

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Application Note

Accelerating Process Optimization in Cell Line Development

This application note describes the power of using the Octet® platform along with Ambr® 15 Cell Culture to identify optimum yield conditions.

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Application Note

A Fast and High Precision Influenza Vaccine Potency Assay

An important understanding of vaccine development process performance, and for correctly scaling each process step.

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Rapid, Automated, At-Line AAV2 Virus Quantitation Advances Bioprocessing in Gene Therapy
Application Note

Rapid, Automated, At-Line AAV2 Virus Quantitation Advances Bioprocessi...

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Application Note

Enhancing Efficiency and Economics in Process Development and Manufact...

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Application Note

Performing Quantitation Assays Using Octet® N1 System

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Application Note

Instant Determination of Protein Presence Using Octet® N1 System

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Application Note

Fast Quantitation of Proteins and Antibodies Using the Octet® N1 Syste...

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Application Note

Reducing Variability in Small Molecule Screening and Kinetics Applicat...

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Application Note

Generating Reliable Kinetic Data For Protein Ligand Interactions

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Optimizing Protein-Protein and Protein-Small Molecule Kinetics Assays
Application Note

Optimizing Protein-Protein and Protein-Small Molecule Kinetics Assays

PDF 950.7 KB
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Application Note

CaptureSelect Affinity Ligands For Antibody Detection And Characteriza...

PDF 1.3 MB
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Octet RH16 with Lab Tech
Application Note

Converting ELISA Assay Into Octet® Quantitation Assay

PDF 207.5 KB
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Application Note

Futureproof your Lab with the Octet®️ R Series of Systems — Flexibilit...

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Brochures (3)

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Brochure

Octet® R Series Systems

Accelerate your research with an upgradeable, label-free protein analysis platform

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Label-free Assays in a Drop (N1 Brochure)
Brochure

Label-free Assays in a Drop (N1 Brochure)

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Octet®️ Systems Platform Brochure
Brochure

Octet® BLI Systems - Enterprise-Ready, Label-Free Biomolecular Interac...

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Case Studies (1)

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Case Study

Bispecific Antibodies: From Research to Clinical Application Case Stud...

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eBooks (8)

Octet®️ BLI 21 CFR Part 11 Compliance Checklist eBook
eBook

GxP Compliance Without the Complexity

A comprehensive tool set for compliance makes the Octet® BLI platform an ideal analytical instrument in regulated environments.

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Label-Free Techniques for Biologics Assessment
eBook

Label-Free Techniques for Biologics Assessment

When investigating biological mechanisms and developing new therapeutics, BLI and SPR offer unique advantages

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eBook

Rapid and Efficient Measurement of Critical Quality Attributes and Par...

The Octet®️ Bio-Layer Interferometry (BLI) platform offers an excellent alternative to traditional assays that are time- and labor-intensive.

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eBook: How Are Label-Free Technologies Used in Early Drug Discovery?
eBook

How Are Label-Free Technologies Used in Early Drug Discovery?

Modern analytical techniques designed for real-time analysis and high-throughput capabilities significantly reduce the time to the discovery

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eBook

Label-Free Detection

Technologies, Key Considerations, and Applications

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eBook

Accelerating the Development and Manufacture of Therapeutics

Establish specificity, accuracy, precision, linearity and range as stipulated by USP 1032 requirements

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eBook

Overcoming the Challenges of Infectious Diseases

Explore the latest methodologies and strategies in the development of future vaccines fighting infectious diseases with label-free approaches

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eBook

Accelerating Vaccines and Virus Research

Live Cell Analysis, Advanced Flow Cytometry and Label-Free Binding Analysis

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Editorials (2)

Editorial

Addressing the Challenges of QC Labs in Vaccine Manufacturing

In cell culture-based vaccine manufacturing processes, microbial contamination control and impurity testing are crucial elements of quality control.

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Editorial

Advancing the Development of COVID-19 Neutralizing Antibodies

Learn more about how virus-neutralizing antibodies have proven to be a key tool in the fight against SARS-CoV-2.

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Flyers (6)

Flyer

Octet® AT: For Octet® BLI Biosensor Transfer

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Flyer

AAV Capsid Titer Data 80% Faster Compared to ELISA

Bioprocess Five Additional Batches of AAV Samples Annually

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Octet service plan
Flyer

Octet® Service Contracts - Protect Your Octet®, Pioneer and BLItz® Sys...

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Flyer

New Octet®️ R Series of Systems Flyer

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Octet top 10 reasons to choose BLI
Flyer

Top Ten Reasons to Choose Sartorius Octet®️ BLI Technology to Accelera...

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selection guide
Flyer

Octet® BLI Biosensor Selection Guide

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Infographics & Posters (6)

Flyer

AAV Capsid Titer Data 80% Faster Compared to ELISA

Bioprocess Five Additional Batches of AAV Samples Annually

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Infographic
Infographic

Understanding Covid-19 Vaccine and Therapeutic DevelopmenT

Learn more about how Octet® systems are used to advance Coronavirus research and vaccine development

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Poster

Influenza Vaccine Titre Determination Bio-Layer Interferometry (BLI)

Fast, accurate determination of vaccine titre during influenza vaccine manufacture is important in understanding process performance and correctly sca...

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Infographic

Octet® Bio-Layer Interferometry (BLI) Technology

Label-free alternative for characterizing protein-protein and drug-protein interactions in standard microtiter plate format

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Poster

High Productivity and Process Economy in GxP Applications

Quantitation assays must show precision, accuracy, linearity, and reproducibility

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Poster

Octet® BLI Systems

Unmatched Versatility for Discovery, Development and Quality Control

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Technical Notes (31)

Technical Note

Still Analyzing AAV Empty/Full Ratio the Hard Way?

Experience a novel, rapid, and high-throughput approach for determining AAV empty/full ratio in as little as 30 minutes.

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Technical Note

Batch Immobilization of a Biotinylated Ligand onto Streptavidin Biosen...

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Technical Note

Octet® Kinetics Assay Method Development Guideline

Developing kinetics binding assays to establish specificity, accuracy, precision, linearity and range as stipulated by USP 1032

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Technical Note

Octet® AHC2 Biosensors

For Quantitation and Kinetic Characterization of Human Fc-Region Containing Proteins

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Octet® High Precision Streptavidin 2.0 (SAX2) Biosensors
Technical Note

High Precision Streptavidin 2.0 (SAX2) Biosensors

Streptavidin is often deployed as the molecule of choice for covalent linking to solid surfaces for ligand binding assays applications.

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Technical Note

Octet® BLI Quantitation Assays: Method Development Guideline

Design and develop biological assays that conform to USP <1032> for accuracy, precision, linearity and limit of quantitation (LOQ) to be establi...

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CHO Host Cell Protein Detection
Technical Note

CHO Host Cell Protein Detection

Designed for a rapid high-throughput quantitation of residual CHO Host Cell Proteins (HCPs) in bioprocessing samples during production and development...

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Technical Note

Octet® ProL Biosensors

For Quantitation of Whole-Molecule Antibody and Antibody Fragments

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Immunogenicity Assessment of Therapeutic Protein on the Octet®️ BLI Sy...

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Octet®️ BLI 21 CFR Part 11 Software Frequently Asked Questions (FAQ)

Octet®️ BLI 21 CFR Part 11 Software Frequently Asked Questions (FAQ)

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Technical Note

Analysis of FLAG Proteins on the Octet®️ BLI Platform Using SAX or SAX...

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Octet GlyM Kit Technical Note
Technical Note

Octet® GlyM Kit - For High-Throughput Mannose Glycans Screening of Cru...

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Octet AAVX Biosensors for Quantitation of AAV Capsids
Technical Note

Octet® AAVX Biosensors for Quantitation of AAV Capsids

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Small Molecule Binding Kinetics
Technical Note

Small Molecule Binding Kinetics

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Technical Note

Host Cell Protein Detection

PDF 952.5 KB
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Technical Note

Octet® Anti-Mouse IgG Fc Capture (AMC) Biosensors

PDF 678.2 KB
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Amine Reactive Second-Generation (AR2G) Biosensors
Technical Note

Amine Reactive Second-Generation (AR2G) Biosensors

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Octet® Amine Reactive SecondGeneration (AR2G) Reagent Kit technical note
Technical Note

Octet® Amine Reactive Second-Generation (AR2G) Reagent Kit

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Technical Note

Biotinylation of Proteins for Immobilization onto Streptavidin Biosens...

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Octet® GST Biosensor Kinetics Assays technical note
Technical Note

Octet® GST Biosensor Kinetics Assays

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Octet® GST Biosensor Quantitation Assays
Technical Note

Octet® GST Biosensor Quantitation Assays

PDF 600.2 KB
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Octet® NTA Biosensor Kinetic Assays technical note
Technical Note

Octet® NTA Biosensor Kinetic Assays

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Octet® NTA Biosensor Quantitation Assays technical note
Technical Note

Octet® NTA Biosensor Quantitation Assays

PDF 551.2 KB
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Anti-HIS (HIS2) Biosensor Quantitation Assays technical note
Technical Note

Anti-HIS (HIS2) Biosensor Quantitation Assays

PDF 630.9 KB
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Technical Note

High Precision Streptavidin Biosensor (SAX) Quantitation and Kinetic A...

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Technical Note

Anti-Penta HIS (HIS1K) Biosensors for Label-Free Analysis of HIS-Tagge...

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Rapid Analysis of Fab Fragments and IgG with Anti-Human Fab2G Biosensors
Technical Note

Rapid Analysis of Fab Fragments and IgG with Anti-Human Fab2G Biosenso...

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Residual Protein A Detection Kit
Technical Note

Residual Protein A Detection Kit

PDF 1.8 MB
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Webinars (16)

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Webinar

Harnessing the Power of Multifunctional Antibodies

Discover the cutting-edge advancements in immunotherapy with a focus on multifunctional antibodies for treating autoimmune disorders and cancers.

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Webinar

A New Wave Biosensor Approach for AAV Process Development

REGENXBIO Inc. shares their experience in finding a high-throughput technique for AAV capsid concentration quantification.

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Webinar

Affinity versus Avidity Webinar

The multivalent nature of antibodies can lead to avidity effects that skew binding affinity data. Hear from a protein analytics expert about avoiding...

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Webinar

Antibody-Drug Conjugates (ADCs) Webinar

Simplifying the Characterization of the drug trastuzumab to Kadcyla®️ and Enhertu®️

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Webinar

Reduce Cycle Times for AAV Quantification

See the Octet® AAVX Biosensor in comparison to an in-house assay on the Octet BLI platform.

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drug target review webinar
Webinar

CHO for Accelerated Antibody Development

Explore applications of the CHO antibody simultaneous display and secretion technology

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Webinar

Cell Surface SARS-CoV-2 Nucleocapsid Protein Modulates Host Immunity

Cell surface Nucleocapsid (N) protein may play an important role in host adaptive immunity to SARS-CoV-2

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Webinar

Rapid Characterization and Selection of SARS-CoV-2 Vaccine Candidates

Using Bio-Layer Interferometry Technology, we developed a set of assays to identify antibodies with SARS-CoV-2 reactivity

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Webinar

Bio-Layer Interferometry as a Strategic Platform

To validate covalent proximity inducing small molecules with synthetic tumor immunotherapeutic applications

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Webinar

Novel High-Throughput Glycoanalytics for Biopharmaceuticals

Improve the overall drug discovery process to increase the success rate of preclinical candidates.

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Webinar

Integrating Octet® BLI into Early Antibody Discovery Workflows

Improve the overall drug discovery process to increase the success rate of preclinical candidates

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Webinar

Characterizing Critical Reagents in Support of Drug Development

BLI technology for affinity characterization antibodies can benefit critical reagent workflows

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Webinar

Fragment Based Drug Discovery of Co-Factor Inhibitors for p97

Virtual screening of molecular databases and biochemical/biophysical techniques

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Webinar

Optimers™️ - Future Ready Antibody Mimetics

Discover the new versatile Octet®️ R series and the use of Optimer™️ binders to improve affinity purification of novel biologics

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Webinar

Drug Discovery with High-throughput Biomolecular Interaction Analysis

Learn how Octet® BLI works in therapeutic discovery and development, including common challenges and solutions

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Webinar

Comparability Studies: Minimize Time, Maintain Quality

Developing Methods for Comparability Studies of Therapeutic Monoclonal Antibodies

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White Papers (8)

White Paper

White Paper: Facilitating Analytical Characterization of Biosimilars D...

Orthogonal Analysis Using Ligand Binding Kinetics and Live Cell Binding

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Key Considerations for Automating Octet® Assays: Ligand Screening at Avitide
White Paper

Automating Octet® Assays for Ligand Screening

Drug development and production is challenging. Avitide, based in Lebanon, New Hampshire, provides on-demand development and supply of high-performanc...

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End of End-Point Assays cover
White Paper

The End of End-Point Assays

Faster time-to results allows assessment of accurate and precise data earlier in the workflow

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Investigating Synthetic Immune Recruitment by Proximity Inducing Molecules - White Paper
White Paper

Investigating Synthetic Immune Recruitment

by Proximity Inducing Molecules

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White Paper

Streamlining Affinity Analysis for Accelerated Lead Screening

Antibody and other protein therapeutics are a major focus in drug discovery pipelines today

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Writing Grant Proposals for Biomolecular Interactions Research

Tips collected over the years from customers successful with their equipment grant proposals.

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White Paper

Enhanced Productivity and Labor Efficiency

In Lot Release and In-Process Testing of Biologics in GxP Laboratories

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Octet Instrument Comparability Kinetics Precision Assessment
White Paper

Instrument Comparability Assessment: Kinetics Precision Assessment in...

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Simplifying Process

Octet R8e brochure on an iPad screen within a scientific lab
Brochure

Octet® R8e BLI System

Discover with Accuracy: Enhanced BLI Analytics With New Heights of Sensitivity

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Quick Start Guides

Product Guide

Octet® BLI Systems Practical Guide

Gain easy and fast access with step-by-step instructions for system preparation, assay optimization, and data analysis, ensuring reliable results.

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Product Guide

Octet® BLI Systems Beginner's Guide

Our beginner-friendly starting guide is your perfect companion, directing you through every step from system setup to data analysis.

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Introducing the New Octet® ARC Biosensors

Octet ARC Biosensors Pouch for Kinetic and Quantitation Analysis - Product Image

Reliable and Versatile Solution for Characterization of RbIgG Proteins

Octet® ARC Biosensors offer a flexible platform for a broad range of high-throughput applications including lead identification and optimization, cell line development, process development, and quality control.

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Biolayer Interferometry (BLI)

Experience the difference of label-free Octet® BLI Systems

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Octet® Publication Database

Literature references covering a large variety of research areas and applications

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