Biophysical Characterization of the Binding of SARS-CoV-2 to the ACE2 Protein Using the Octet^® R4 Bio-Layer Interferometry Platform Application Note

Overview

Molecular level studies are routinely used to understand the determinants of infectivity for viruses, such as the binding interaction between SARS-CoV-2 to cells of respiratory mucosa through the angiotensin converting enzyme 2 (ACE2) receptors in the human body.

In this application note, we use the Octet^® Bio-Layer Interferometry (BLI) platform to characterize the binding of SARS-CoV-2 to the various molecules engaged during an infection (ACE2 protein, spike protein-S, receptor binding domain and the trimeric SARS-CoV-2 S protein). See how this advanced system allows you to quickly evaluate the thermodynamics at play during molecular interactions.