CHO Host Cell Protein Detection

This technical note outlines a protocol for using the Sartorius-Cygnus Anti-CHO HCP Detection Kit in developing and routine running of process-independent assays on the Octet platform. The measurement involves a sandwich-type assay on an Anti-CHO HCP Biosensor that is pre-coated with the gold-standard 3G Anti-CHO HCP antibody from Cygnus Technologies. 

A completely hands-off, walk-away HCP assay analyzing 96 samples can be set up to run automatically on an Octet HTX instrument with results obtained in one hour. The assay can also be run on other 8- and 16-channel Octet instruments together with the Sidekick Station with time-to-results of 75 and 90 minutes, respectively.