Antigen Expression & Selection

After virus-host interaction studies, candidate antigens and vaccines undergo thorough evaluation and characterization of antibody binding, antibody epitope binning as well as vaccine titer and potency assays.

See how Sartorius' innovative virology solutions advance and accelerate these key applications in discovery and development of new vaccines against viral infections.

Cell and Protein Analysis Platforms - For tracking complex host - pathogen biological processes, you need high-capacity cell analysis solutions to generate deeper, more relevant data on phenotype, activation, and function.

Antigen, Antibody Expression - For progressing with your leads rapidly through the pipeline you need high throughput, multi-parallel cell culture systems.

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Antigen Expression & Selection Categories

Epitope Binning

Assess Epitope Diversity and Coverage Rapidly categorize and bin antibodies that bind to similar epitope regions using Octet® cross-competition assays...

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Titers and Potency: Fast Vaccine Development

Fast and accurate determination of vaccine titer in cell culture is important in understanding vaccine development process performance and for correct...

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Cell Health and Viability Assays

Study virus-modulated cell health, proliferation and viability in real time. Cell-by-cell analysis can help you monitor and visualize changes in the p...

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Antibody Titer and Screening

Identify antibodies binding to desired antigens, transfected cells or natural expressors. Rapidly identify clones with specific binding profiles by ut...

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Multiparallel Small Scale Cell Culture

High-throughput cell culture systems with scalable, multiparallel mini-bioreactors and automated functionality accelerate cell line/strain selection.

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Mycoplasma Detection

Human, animal and insect cells are commonly used in cell culture-based processes and are typically susceptible to mycoplasma infections. Sartorius’ Mi...

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Sartorius Antigen Expression & Selection Solutions Offer:

Fast, Flexible Antibody Epitope Characterization Platform

A study showed a non-competing pair of human neutralizing antibodies blocked the binding of SARS-CoV-2 to its ACE2 receptor. In this research the Octet® system was used to investigate antibody binding.

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Vaccine Research-Tailored Biosensors for Efficient Workflow

Select from a range of tailored affinity capture and immobilization chemistries suitable for vaccine and biotherapeutic development research.

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Accuracy and Reproducibility in Titer and Screening Measurements

Using the iQue® platform, researchers assessed antibody binding to native spike protein in SARS-CoV-2 research. This research helped to highlight a potential therapeutic route for COVID-19 patients.

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Product Highlights for Antigen Expression & Selection

Octet® Bio-Layer Interferometry

Octet® BLI systems provide researchers with the capability to detect the interactions of a diverse range of biomolecules, from small molecules to proteins and viruses. It is widely used for determining the kinetics and affinity of host-pathogen binding interactions.

  • Real-time binding kinetic and affinity measurements
  • Measure interactions from purified and crude samples
  • Fast data acquisition — simultaneously data from up to 96 biosensors

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Incucyte® Live Cell Analysis uses whole-well, label-free imaging to scan clones and verify monoclonality.

Incucyte® Real-Time Live-Cell Analysis

The Incucyte® Live-Cell Analysis Systems automatically capture and analyze images from multiple microwell plates in parallel, thereby significantly increasing throughput. The systems enable acquisition and analysis of data throughout the course of an experiment to capture time-dependent effects, which is in contrast to endpoint analysis by traditional cell culture techniques. The mechanism of infection can be studied by assessing virus-modulated cell death and proliferation.

  • Real-time continuous kinetic measurements
  • Never miss a data point
  • Networked remote access reduces risk of contamination
  • Monitor viral infection, cell health, movement, morphology, and function

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iQue Advanced Flow Cytometry Platform

Que® Advanced Flow Cytometry

The iQue® Advanced Flow Cytometry Platform gets you from samples to actionable results in record time. The iQue® platform can be used for viral vaccine research and virology studies such as antibody library screening, immune response assessment, including epitope mapping and antibody neutralization studies.

  • High-throughput multiplexed cytometry screening and real-time data analysis
  • Sample as little as 1 ul from miniaturized assay volumes
  • Multiplex cell phenotypes and cytokines in the same well

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Antigen Expression & Selection FAQs

Epitope binning assays with Octet® BLI instruments help identify antibodies that block the same epitope on a target antigen and are crucial when it comes to identifying or engineering mAbs with favorable kinetics and affinity profiles. This assay format can also be used to determine neutralization antibody competition with virus receptor binding to host receptors.

Octet® BLI biosensor assays are used for clone screening, titer analysis and relative screening for determining other critical quality attributes (CQAs) such as potency or relative screening of the glycosylation profile of biologics drug molecules. Crude sample compatibility of Octet® BLI assays enable early analysis of clone specificities, productivity and attributes for early decision making.

Yes, the iQue Advanced Flow Cytometry bead-based kits enable the multiplexed assessment of antibodies binding to different SASR-CoV-2 proteins and isotypes simultaneously. iQue Qbeads® DevScreen SAv, part of the Qbeads® DevScreen family, are bead-based kits that provide you more flexibility to make your own bead-based multiplex assays.

iQue Qbeads® DevScreen SAv are streptavidin coated beads that can be used to screen with biotinylated targets. There are 5 different SAv-coated bead populations, and these can be multiplexed.

Yes, Humane Genomics used this technology to examine the binding of a vaccine candidate to ACE2 receptors on target cells. In the video, the ACE2 receptor (red) on the cells is shown binding with the SARS-CoV-2 spike protein (green) on the vaccine. This binding is visualized as yellow, co-localized patches. Infectious Diseases.

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To minimize the risk of contamination, it is recommended to implement a number of measures, such as aseptic working, use of mycoplasma-free tested reagents and media, and regular mycoplasma testing. In addition, filtration of media prepared from dry media is an essential element in a prevention strategy. Our vacuum bottle-top filters Sartolab® RF with a pore size of 0.1 μm can effectively remove the mycoplasma strains A. laidlawii and M. hyorhinis.

Antigen Expression & Selection Resources

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Real-Time Live-Cell Analysis of Lentiviral Titer Determination

This application note describes the utility of the Incucyte® Live-Cell Analysis System in combination with Incucyte® Fabfluor-488 Antibody Labeling an...

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Webinar: A Dissection of SARS-CoV-2 with Primary Human Lung Culture Sy...

Join Pharma IQ and The Sartorius Group for an overview of the methods for human lung organoids and quantitative assessment of the biological effects o...

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A Fast and High Precision Influenza Vaccine Potency Assay

A Fast and High Precision Influenza Vaccine Potency Assay

The Octet® platform’s Bio-Layer Interferometry (BLI) technology offers an alternative assay to the Single Radial Immunodiffusion (SRID) technique that...

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