Translate Insights into Novel Strategies for Virology and Viral Vaccine Research
Innovative Solutions for Extraordinary Times
Since the global outbreak of the COVID-19 pandemic, remarkable progress has been made in vaccine R&D, accelerated by unprecedented collaboration in the scientific world. Emergence of virus variants, however, reminds us we need a deeper understanding of the cellular and molecular mechanisms of virus-host interactions that drive immune responses against the virus and its variants. To enable these critical investigations, Sartorius offers live-cell analysis, advanced flow cytometry and label-free binding analysis platforms.
- Cell and Protein Analysis Platforms - For tracking complex host-pathogen biology, you need high-capacity cell and protein analysis solutions to generate deeper, more relevant data on phenotype, activation and function.
- Cell Culture - For progressing your leads rapidly through the pipeline, you need high-throughput, multi-parallel cell culture systems.
- Downstream Applications – Overcome bottlenecks in lab scale downstream process, like clarification, concentration and purification of proteins and viruses.
Lab Solutions for Virology & Vaccine Research
Sartorius Virology and Vaccine Research Solution Offer:
Perform rapid, multiplexed, evaluation of immune-cell function through simultaneous analysis of cell-mediated cytotoxicity, cell health, function, activation, phenotyping and cytokine profiling.
Rapidly categorize and bin antibodies that bind to similar epitope regions using Octet® cross-competition assays.
Acquire and analyze data throughout the course of an experiment to capture time-dependent effects.
Collapse vaccine harvesting workflows to a single-step clarification and sterile filtration. Streamline your virus and protein purification and concentration workflows with ultrafiltration devices offering a broad range of membrane types and molecular weight cut-offs (MWCO).
Product Highlights for Lab Solutions for Virology & Vaccine Research
Octet® Bio-Layer Interferometry
Octet® BLI label-free binding and concentration measurement assays are widely used in vaccine and therapeutics development programs because they quickly provide binding information critical to the lead selection process. Traditional methods for analyzing biomolecular interactions such as ELISA provide only end-point data not taking into consideration association and dissociation kinetics. Furthermore, they are often cumbersome and may require rigorous sample preparation.
- Real-time binding kinetic and affinity measurements
- Measure interactions from purified and crude samples
- Fast data acquisition — simultaneously data from up to 96 biosensors
iQue® Advanced Flow Cytometry
The advanced iQue® flow cytometry platform enables high-throughput immune-cell profiling and functional assessment, immune cell activation and antibody screening with minimal sample volume and rapid results, in real time and without compromising your cell cultures.
- High-throughput multiplexed cytometry screening and real-time data analysis
- Sample as little as 1 ul from miniaturized assay volumes
- Multiplex cell phenotypes and cytokines in the same well
Incucyte® Real-Time Live-Cell Analysis
The Incucyte® Live-Cell Analysis Systems can automatically capture and analyze images from multiple microwell plates in parallel, thereby significantly increasing throughput. The systems enable acquisition and analysis of data throughout the course of an experiment to capture time-dependent effects, which is in contrast to endpoint analysis by traditional cell culture techniques.
- Real-time continuous kinetic measurements
- Never miss a data point
- Networked remote access reduces risk of contamination
- Monitor viral infection, cell health, movement, morphology, and function
Lab Solutions for Virology & Vaccine Research FAQs
The Incucyte Live-Cell Analysis System offers an automated option for working with pathogenic microorganisms while they remain in the incubator undisturbed for greater biological relevance. Using simple and reliable quantification and non-perturbing reagents, Incucyte® enables real-time analysis of viral infection and replication, host interactions, cell viability and antiviral screening, providing a platform to enable antiviral discovery for new treatments and vaccine development.
The Qbead® family of iQue® reagents enables capture of specific proteins on distinct beads to facilitate multiplexed quantitation of a range of proteins, cytokines, enzymes, growth factors, etc. in a single well at microliter assay volumes.
Target binding characterization is an essential analytical step for the selection of high-affinity and highly specific therapeutics, regardless of molecule type. Confirming the binding results, kinetic analysis with Octet® BLI instrument further describes the components of association and dissociation that comprise the overall affinity interaction.
Yes, the Octet® systems with a range of different biosensor chemistries offer robust assays for antibody and virus titer (ex. Influenza), potency and stability determination.
How can I easily clarify my small-scale cell cultures to obtain viruses and proteins for downstream analysis?
Sartoclear Dynamics® Lab kits are designed for rapid harvesting 15 mL to 1,000 mL volumes of cell cultures in the lab, enabling clarification and sterile filtration to be performed in one step. Sartolab® Multistation enables the hands-free and simultaneous filtration of up to 6 samples of small volumes.
How do you differentiate between Avidity and Affinity and how can one avoid avidity effects on a label-free affinity assay?
While Affinity measurements are typically a result of a 1:1 interaction between an antibody and it’s respective binding partner (antigen), avidity typically results from multiple affinities originating from two or more binding sites. Avidity is often symbolized by an artificially slower dissociate step that emanates from secondary binding that could occur due to nonoptimal assay design. To avoid avidity effects, an optimal assay design where ligand loading is well optimized is necessary. The most optimal assay design is to use the antibody as the ligand while the antigen stays in solution. If the antigen must be kept in solution, a dip and read plate-based platform such as the Octet, allows scientists to rapidly screen for optimal ligand loading concentrations (vary ligand while keeping the analyte (antibody) concentration constant and assessing optimal loading using the dissociation step profile.
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The CellCelector platform is a unique and fully automated cell imaging and picking system developed for detection, selection and isolation of single cells, clusters, spheroids and organoids as well as single cell clones and adherent colonies. It is widely used in a multitude of research areas such as:
- Antibody Discovery
- Cell Line Development
- Isolation of Bacterial Colonies Workflows and more