Downstream Vaccine Research
Vaccine research and development requires downstream process techniques to obtain the target antigen molecule and prepare the vaccine candidate for further analysis. The downstream process starts with the clarification and sterile filtration of small-scale cell cultures to obtain viruses and proteins in the supernatant, which is followed by concentration and purification of the molecules. This process can be very tedious and time-consuming.
To overcome this bottleneck, Sartorius offers rapid, user-friendly and scalable solutions to help you simplify cumbersome downstream application steps.
Streamlining the Workflow From Cell Culture Clarification to Concentration and Purification of Proteins and Viruses
Sartorius Downstream Vaccine Research Solutions Offer:
Sartoclear Dynamics® Lab kits are designed for rapidly harvesting 15 mL to 1,000 mL volumes of cell culture in the lab, enabling clarification and sterile filtration in one step. Sartolab® Multistation enables hands-free and simultaneous filtration of up to six samples of small volumes.
Vivaspin® centrifugal concentrators provide near-total mAb, protein, virus and other nanoparticle recoveries, while achieving high concentration factors in the shortest time. Now, we offer both PES and RC membranes to optimize molecule-to-membrane compatibility.
- Unique PES and RC membrane range
- Fastest concentration times
- Angular dead stop for complete sample retrieval
- Market-leading technical and application support
Measuring vaccine potency or biologically active components is critical to the determination of a vaccine’s effective dose. In addition, the stability of the vaccine has a major impact on its usage for immunization programs worldwide. Octet® Bio-Layer Interferometry (BLI) systems deliver comprehensive characterization of vaccines as well as rapid and reproducible determination of protein concentrations for titer and functional activity assays. A single platform delivers high value across a wide range of applications in vaccine discovery, development and manufacturing.
Product Highlights for Downstream Vaccine Research
Clarification and Filtration
Sartoclear Dynamics® Lab kits: Virus and Protein Harvesting in One Step
Sartoclear Dynamics® Lab kits are designed for rapid harvesting 15 mL to 1,000 mL volumes of cell cultures in the lab, enabling clarification and sterile filtration to be performed in one step. Kits combining a vacuum filtration unit, Sartolab® RF with a PES membrane and a filter aid for clarification.
Concentration and Purification of Viruses
Vivapure® Virus Purification and Concentration Kits
Adenovirus and lentivirus purification kits utilize membrane adsorber technology for fast and safe virus purification. High yields with low endotoxin levels are ideal for downstream use in cell-based assays.
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Concentration, Purification and Diafiltration of Proteins (Antigens and Antibodies)
Vivaflow®, Vivaspin® and Vivapure® Ultrafilters
Ultrafiltration devices offer perfect solutions for ultrafiltration needs in protein, peptide, nanoparticle and virus purification workflows. From concentration and diafiltration of the target molecule source (such as a cell culture supernatant) to final concentration, desalting or buffer exchange of the purified protein.
See how Vivaspin® was used in recent Coronavirus related research experiments for concentration of Virus-Like Particles (VLPs) and proteins.
- Evidence that TMPRSS2 Activates the Severe Acute Respiratory Syndrome Coronavirus Spike Protein for Membrane Fusion and Reduces Viral Control by the Humoral Immune Response. Glowacka et al. Journal of Virology
- Expression, purification, and crystallization of the SARS-CoV macro domain. Malet et al. Crystallography Journals Online
- Structure of the SARS-Unique Domain C From the Bat Coronavirus HKU4. Staup et al. SAGE Journals
- Structure, Function, and Antigenicity of the SARSCoV-2 Spike Glycoprotein. Walls et al. Cell
Cost-Saving Characterization of Vaccines and Biologics
Label-free Octet® BLI technology is used in downstream processing to determine titer, potency, and stability of biologics and vaccines.
Cubis® II Modular Premium Laboratory Balances
Cubis® II for Vaccine Development
Sample weighing is a common step during vaccine research and development, and in pre-clinical phases where reliable lab weighing results are very importance. As vaccine research becomes more challenging with the continuous emergence of new diseases and novel safety concerns, higher standards of precision, as well as flexibility and speed, are needed.
Sterility Testing of Sterile Pharmaceuticals
Closed Membrane Filtration Systems for Sterility Testing
Our Sterisart® portfolio is easy to use, minimizes the risk of false positives and false negative, meets all regulatory requirements and provides you with complete confidence in your sterility testing results. We redefine industry standards.
Picus® 2 Electronic Pipette - Connected for the Future with Bluetooth Connectivity
The Picus® 2 pipettes ensure reliable and repeatable pipetting results and feature an unbeatable ergonomic design that is kind to your hand. Anyone can use Picus® 2; it is intuitive like a mechanical pipette, while also offering more advanced options for experienced users.
Microsart® AMP Mycoplasma Kit
Ensure your results are from your cells, not from contamination. Screen your cell cultures with our real-time PCR kit for mycoplasma contamination.
Arium® Ultrapure Water Systems
Designed for efficient removal endotoxins, RNase, DNase, microorganisms and particles. Provides the highest safety for your critical cell culture applications.
Blotting Papers
Soak it up right! Pure blotting paper and consistent CN membrane for reproducible results.
Downstream Vaccine Research FAQs
Yes, Sartoclear Dynamics® Lab Kits with filter aid offer fast and effortless solution for clarification of sample volumes of up to 1L, enabling clarification and sterile filtration to be performed in one step.
It can! Sartorius provides a convenient conversion table to choose a MWCO up to 1/3 the diameter of the target virus. For example, high recoveries of Lentivirus - which has a diameter of ~90 nm - are achieved with 300 kDa MWCO Hydrosart® or 100 kDa MWCO PES membranes.
Ultrafiltration can be used for concentration, diafiltration, washing or purification of nanoparticles. In addition, ultrafiltration can be used to separate free- from nanoparticle-bound or encapsulated APIs. This enables a detailed understanding of nanoparticle functionalization, informing correct dosing while minimizing toxic effects.
Sartorius offers a comprehensive range of lab ultrafiltration solutions to meet the research and development needs for nanoparticles which will be used in tomorrow’s nanomedicines. Centrifugal, pressurized and crossflow devices enable processing of 0.1 mL to 5 L samples, offer superior process speeds compared to conventional techniques, and provide high target recoveries.
Use of centrifugal concentrators for buffer exchange or sample concentration requires optimization of the protocol and correct handling of the device in conjunction with a pipette to ensure:
- Determination of the concentrated sample volume for accurate yield measurement
- Retrieve the full volume of sample in a tall concentrator tube while avoiding sample-to-pipette and pipette-to-sample contamination
- Proper rinsing so that the preservatives used to maintain membrane stability do not elute into the sample leading to anomalous peaks in concentrate analysis
Read Effectively Use Picus® Pipettes with Vivaspin® Concentrators Application Note
How does the quality of ultrapure water influence the data and reproducibility of virology research?
Many of the assays used in vaccine and virology research are vulnerable to interference and contamination. Ensuring the availability of RNase/DNase and endotoxin-free water is essential for accuracy. In addition, the purity of laboratory water can influence the quality of viral pseudotypes, with demineralized water significantly decreasing transduction capacity compared to ultrapure water.
Arium® water systems offer ultrapure water solutions suitable for sensitive analytical workflows such as those used in virology and vaccine research. Download the Arium Virus Research Application Note
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