Lead Selection & Optimization

Lead Selection & Optimization

Lead Molecule Selection and Optimization

Lead selection and optimization is a critical process in the identification of molecules that meet predefined requirements and can be progressed to the next step of development. While biologics lead selection mainly involves the screening of lead biological molecules to select those with desired functional and biophysical characteristics, optimization helps improve these properties. 

Octet® BLI systems are improving the speed and efficiency of selection and optimization workflows with high throughput and easily developed assays for applications like affinity ranking, epitope binning of large antibody matrices, Fc-receptor binding, glycosylation screening, antibody-antigen specificity, binding characterization and titer analysis.

Why Do Researchers Choose Genuine Octet® BLI?

Thousands of researchers trust Octet® BLI systems for superior
performance, confident compliance, and exceptional customer
service & support.

  • 7000+ Octet® BLI publications
  • 3,500+ instrument installs worldwide
  • Much, much more

See Why Researchers Choose Octet® BLI

Featured Applications

Titer Determination on the Octet® BLI Platform 

Titer and protein concentration determination is a critical process in the development of biologics drug molecules. The active protein concentration can be used to determine potency of the drug molecule. Titer and protein concentration determination are more robust to cell culture and media, making them desirable as replacement for enzyme-linked immunosorbent assay (ELISA) and high-performance liquid chromatography (HPLC) in upstream and downstream processes. 

  • Analyze a full 96-well plate of samples for IgG titer in as little as two minutes 
  • Use crude and unpurified samples 
  • Automate for walkaway high throughput analysis

Read Application Note

Epitope Binning and Cross-Competition Assays

In early drug development, cross-competition studies are necessary to characterize hundreds of antibody clones. Since monoclonal antibodies in different bins bind to distinct antigen epitopes and display diverse functional characteristics, epitope binning studies can increase the likelihood of choosing a lead antibody with the desired biological activity. 

Epitope binning studies rely on the sequential binding of two antibodies to an antigen, and are performed using dozens of antibody pairs in cross-competition matrices. The Octet® BLI platform excels at these large-scale studies due to assay speed, throughput and exceptional reproducibility. 

  • Sample plate format allows for use of crude and non-purified samples
  • Automation capable Octet® RH96 and RH16 allow for walk away high throughput analysis

Read Application Note

Off-Rate Screening Utilizing Octet® BLI technology

When screening crude samples with unknown concentrations, off-rate screening is a powerful tool for predicting sample properties. Library screening by ELISA does not enable ranking of antibodies based upon their affinities for an antigen. Using Octet® BLI systems, clones with high affinities and low off-rates can be rapidly identified and selected for further characterization. Many biotechnology companies utilize the technology in automated affinity screening and off-rate screening of positive clones obtained from ELISA-based primary screens.

Read More

Kinetic Characterization and Clone Selection 

Monitoring the binding of candidate molecules to targets is a critical process in lead molecule selection and can aid in the selection of desirable clones. Octet® BLI systems generate highly-precise data on molecular binding affinities, specificities and association/dissociation rate constants and in a high throughput manner. 

  • Accurately determine ka, kd, and KD
  • Screen up to 96 clones simultaneously in 96 or 384-well plates
  • Perform analysis directly in crude samples - no need for sample purification

Read More

Fragment Screening

Fragment-based drug design (FBDD) has become an increasingly popular platform for the identification of lead candidates in drug discovery programs. The detection and characterization of fragment binding events is facilitated by sensitive biophysical technologies capable of detecting low affinity interactions of low molecular weight compounds. The Octet® SF3 system has the necessary sensitivity and throughput to provide complete fragment screens on libraries of several thousand compounds in just a few weeks per target.

Read Application Note

Find Out How You Can Leverage the Power of Octet®️

Boost your research and commercialization goals by combining BLI and SPR at an attractive promotional price. 

Explore Promotion

Featured Resources

Key Considerations for Automating Octet® Assays: Ligand Screening at Avitide
White Paper

Automating Octet® Assays for Ligand Screening

Drug development and production is challenging. Avitide, based in Lebanon, New Hampshire, provides on-demand development and supply of high-performanc...

Read White Paper
A compendium for successful BLI and SPR assays
Application Guide

A Compendium for Successful BLI and SPR Assays

Octet® Label-Free Biosensor Analysis

Access the Guide

Integrating Octet® BLI into Early Antibody Discovery Workflows

Improve the overall drug discovery process to increase the success rate of preclinical candidates

Access Webinar
app note cover
Application Note

Customized Quantitation of Recombinant Therapeutic Proteins

Using Octet® SAX Biosensors

Access Application Note
A Rapid Method to Quantitatively Screen Bispecific Antibodies Using Protein A and Octet® His1K Biosensors
Application Note

Screen Bispecific Antibody Using Protein A and His1K Biosensors

Rapid quantitative screening of bispecific antibodies using Protein A and HIS1K biosensors

Access Application Note
Cover: Characterizing Membrane Protein Interactions by Bio-Layer Interferometry
Application Guide

Characterizing Membrane Protein Interactions

By Octet® Bio-Layer Interferometry (BLI) Platforms

Access Guide
Application Note cover
Application Guide

Label-Free Technologies

For Accurate Determination of Kinetics and Affinity Rate Constants

Access Guide
White Paper

Streamlining Affinity Analysis for Accelerated Lead Screening

Antibody and other protein therapeutics are a major focus in drug discovery pipelines today

Access White Paper
Cell Line Development: Accelerating Antibody Discovery by Monitoring Titer and Glycosylation With the Octet® Platform
Application Guide

Cell Line Development

Accelerating Antibody Discovery by Monitoring Titer and Glycosylation With the Octet® Platform

Access Guide

Instant Access

app note cover
Application Note

Enhancing Efficiency and Economics in Process Development and Manufacturing of Biotherapeutics

PDF | 372.5 KB
app note cover
Application Note

Reducing Variability in Small Molecule Screening and Kinetics Applications

PDF | 1.4 MB
Kinetic Analysis of Antibody Binding to an Expressed Membrane Protein on Captured Lipoparticles
Application Note

Kinetic Analysis of Antibody Binding to an Expressed Membrane Protein on Captured Lipoparticles

PDF | 929.6 KB
SPR Fragment based drug discovery
Application Note

Fragment-Based Drug Discovery (FBDD) Using Octet® SPR OneStep® and NeXtStep™ Injections

PDF | 5.1 MB
Octet® SF3 SPR

Octet® SF3 SPR 

Generate high-quality kinetics and affinity data in a fraction of the time compared to standard multi-cycle kinetics using the Octet® SF3.

Explore Octet® SF3
Octet® BLI rapidly determines recombinant protein concentration as a control parameter in cell line development.

96-Channel Ultra High Throughput Octet System: Octet® RH96

Monitors up to 96 biosensors simultaneously, enabling label-free detection for protein quantitation and kinetic characterization at unmatched speed

Explore Octet® RH96
Octet RH16 ultra high throughput label free detection system

16-Channel High Throughput Octet System: Octet® RH16

Ideal for high-throughput applications that demand high sensitivity and low sample volume requirements

Explore Octet® RH16
Octet R8 label free detection system instrument

8-Channel System: Octet® R8

Unmatched Flexibility and Versatility in Protein Analysis

Explore Octet® R8

Request a Quote, Demo, or More Information

What other areas are you interested in? (select all that apply)

Request a Quote, Demo, or More Information

Related Content


Biologics Characterization Tools

Explore More

Octet® System Service and Support

Explore More

Octet® BLI Resources

Explore More