s

mAb Molecule Development - Lead Optimization

mAb Lead Optimization

Before moving forward with your early drug candidates, you need deeper, more meaningful insights and information-rich data about their characteristics. You need data from your candidates, such as 

  • Minimal immunogenicity profile 
  • Target specificity, selectivity and optimal effector functions

Assess these properties of your mAb candidates with phenotypic and functional assays from Sartorius.

Contact us

NEW INFOGRAPHIC

Challenges and Solutions for Developing New Monoclonal Antibodies

Unprecedented advances in medicine are delivering remarkable results for some patients. Recent approvals of CAR T therapies and new approaches in regenerative medicine, for example, exemplify Monoclonal antibodies (mAbs) used for immunotherapy stimulate the immune system to mount a response to target antigens. These powerful therapeutics are being used to...

Download to learn more

WEBINAR

Accelerating Nanobody Discovery Workflows with High-Throughput Flow Cytometry

In this webinar, Dr. Pieter Kennis discusses how nanobodies – small, single-domain antibody fragments that retain full antigen-binding capabilities and the properties of heavy-chain antibodies – and nanobody discovery and screening platforms exploit high-throughput flow cytometry to increase the throughput of nanobody screening campaigns.

Register to Watch Now
 


Intellicyt® iQue Screener PLUS

Functional Profiling

Perform multiplexed assays for functional analysis in antibody engineering/mutation/humanization screens with the iQue Screener PLUS.

Get a better indication of in vivo behavior by detecting target proteins in their native conformation on the cell surface.

Epitope Mapping: Rapidly detect and visualize epitope regions for hundreds of clones with high sensitivity, and validate your data with additional information on cell health and viability— all in one assay.

Learn more

IncuCyte® Live-Cell Analysis System

Study your lead candidates at work!

Get detailed, multiplexed, functional and morphological insight on complex cell models with real-time, automated measurements of the dynamic interplay between immune and cancer cells, directly inside your incubator.

Learn more

Read the article: Investigating new cancer therapy candidates with live-cell imaging

Sartoclear® Dynamics Lab P15 and Lab V

Quickly and easily prepare your clarified supernatant with your target molecule with Sartoclear® Dynamics, a simplified, small-scale, mammalian cell harvesting workflow for further analytics of your antibody lead candidates. 

Rapid and convenient clarification of high density mammalian cell cultures is often challenging. Our solution provides a single-step clarification and sterile filtration protocol--without clogging--that is 70% faster than traditional centrifugation.

Learn more

BioOutsource Testing Services

Physiochemical and Structural Analyses

Characterizing the inherent structural heterogeneity is an essential requirement of the development of a therapeutic monoclonal antibody. High and low molecular weight species, charge variants and post-translational modifications can all affect in vivo stability, loss of biological activity and produce unwanted immune effects.
 
Our team of experienced chemists can provide the support and advice required to ensure that these risks are managed by ensuring protein structure analysis and physiochemical analysis is performed at appropriate stages of the development of a therapeutic antibody.
 

Biological Analysis

Characterize the binding and functional activity of your biologic molecule with a range of cell-based binding and potency assays. Advance the development of your biosimilar with our comprehensive range of assays, available off-the-shelf for over 12 biosimilar target molecules.

Learn more

Application Note: Rapid Mammalian Cell Harvest without Centrifugation for Antibody Purification Using the Sartoclear Dynamics Lab Filtration System

Download now

Resources

Application Note: Minimizing Syringe Filter Usage in harvesting Monoclonal Antibodies from CHO Cell Culture Supernatants

PDF | 204.5 KB

Download

Application Note: Vivaflow® 200: A Critical Sample Preparation Tool for Concentrating Hybridoma Supernatants

PDF | 680.9 KB

Download

Application Note: Concentration of Mammalian Cell Culture Supernatants with Vivaspin® Turbo 15 and Amicon® Ultra-15 Devices

PDF | 138.5 KB

Download

Request More Information

Are you interested in the following cell analysis instruments for your research?

Other areas of interest? (Please check all that apply)

How can we assist you?