Speed Up Your Entire Workflow with iQue® 5 High-Throughput Screening (HTS) Cytometry
Screen a 96-Well Plate in 5 Minutes
Traditional flow cytometers can analyze a limited number of samples but fall short for rapid screens of antibodies or chimeric antigen receptor (CAR) T cells.
The iQue® HTS Cytometer addresses this critical gap as a data generation powerhouse for high-throughput screening applications. Its unique advantages lie in its speed and user-friendliness, from setup to acquisition and analysis, dramatically boosting productivity. Your results will be ready by the time you finish a quick coffee break.
Featuring a patented sampling method, the iQue® enables the fastest sample acquisition in the industry. It supports 96- or 384-well plates and facilitates continuous plate loading through integration with popular robotics systems. Coupled with integrated analysis and novel visualization tools, this platform delivers quality results faster, so you can develop therapeutics sooner.
Choose the Right iQue® HTS Cytometer for Your Needs
Get the Data You Need Faster
- Market-leading speed completes processes 96-well plates in as few as 5 minutes or 384-well plates in 20 minutes
- Validated consumables and buffers ensure consistent performance
- Plug and play kits speed up assay design and validation
- Patented sampling with replaceable probe / tubing reduces downtime from clogs
Design Your Assay Your Way
- Broad detection range covers cells, beads, yeast and more (down to 0.2 µm)
- Up to 27 channel capacity supports wide range of applications
- Flexible throughput by compatibility with 96- and 384-well plates
- Integration with robotics systems allows extended operation
A True User-Friendly Experience
- Intuitive iQue Forecyt® Software simplifies operation for users of all levels
- Multi-user environment features walkaway automation, comprehensive analysis, and advanced visualization tools
- iQue® Qmax trolley provides convenient sheath and waste fluid management during long runtimes
System Overview: iQue® HTS Cytometer
Explore the Wide Range of Supported Applications
Antibody Discovery and Development
Adoptive Cell Therapy
Small Molecule Screening
- A simple, scalable, multi-user environment featuring walkaway automation, comprehensive analysis and visualization tools that are not available in traditional flow cytometers
- Walk-up-and-use operation that eliminates the need for pre-analytical user setup and power users
- Flexible plate formats suitable for a broad range of cell and bead-based analyses
Rapid, Walkaway Processing of 96 or 384 Well Plates
- Patented, rapid microvolume sampling processes 96 well plates in as few as 5 minutes or 384 well plates in 20 minutes
- Samples are delivered to detectors in an air-gap delimited stream, so an entire plate of data can be processed at one time
- Generous cartridge station allows for unattended multiple plate processing
Simultaneous Cell Phenotyping and Secreted Protein Detection
Real-time Data Acquisition and Analysis and Dynamic Visualization of Results
- Rapid assay optimization – adjust a gate to see updated plate-wide analysis in real time
- Easy, interactive assay development and decision environment that is reproducible across plates
- Plate level annotation, analysis, and visualization integrated within the standard population selection tools
HTS by Cytometry Features
Biologically Relevant, Reproducible, and Cost Effective HTS Cytometry
Relieve capacity bottlenecks throughout your entire workflow with faster plate sampling, integrated analysis, and novel data streamlining tools.
Enjoy an easy to use, multi-user environment with walkaway automation, including automated QC bead vortexing on-board and 48-hour continuous run times.
Take advantage of the open platform design for integration with leading robotic systems for automatic plate handling and operation.
Rely on our experts to overcome hardware, software, or technical challenges and get your experiments back on track as fast as possible.
See how your peers are accelerating their studies with the latest iQue® research, featuring 1,200 + references in peer-reviewed publications.
High-Throughput Screening (HTS) by Cytometry Software, Reagents, and Consumables
High-Throughput Screening (HTS) by Cytometry Software
iQue Forecyt® Software —Rapid Data Visualization and Interpretation
The integrated iQue Forecyt® software addresses the analysis bottlenecks associated with multi-parametric data generation by enabling rapid data visualization and interpretation.
Reduce data analysis times from days to minutes by having all of your data analyzed as soon as the plate is done sampling.
Flow Cytometry Reagents & Consumables
Optimized for the iQue® HTS Platform
iQue® Kits, most of which have been developed to work with no-wash protocols, are packaged to suit a wide range of throughput needs.
These kits, in conjunction with an iQue® HTS Cytometer and iQue Forecyt® Software, provide an integrated screening solution enabling high content, high throughput screening of cells and beads for deeper insight into complex biologies.
iQue® Specifications & Optical Configurations
The iQue® is Available In Multiple Optical Configurations and Flexible Plate Formats
The iQue® HTS by Cytometry Platform is available in 4 configurations to address a wide range of screening and experimental applications. The entry level 2 laser iQue® 3 BR is equipped with a blue and a red laser ideal for applications which do not require many parameters, but, can take advantage of the speed the iQue® HTS Platform is famous for.
3 laser configurations increase the flexibility and accommodate larger multiplexing needs depending on the specific fluorphores. The iQue® 3 VBR includes Violet, Blue, and Red lasers, while, the iQue® 3 VYB replaces the red laser with a yellow laser.
The iQue® 5 further expands iQue® capabilities, with its 4 (Violet–Yellow-Blue–Red) laser and 27 channel configurations.
| iQue® 3 Blue-Red (BR) | iQue® 3 Violet-Blue-Red (VBR) | iQue® 3 Violet-Yellow-Blue (VYB) | iQue® 5 Violet – Yellow -Blue – Red | |
| Catalog No. | 91375, 91381 (HD) | 91377 | 91379 | BA-97135 |
| Lasers | 488nm, 640nm | 405nm, 488nm, 640nm | 405nm, 561nm, 488nm | 405 nm, 488 nm, 561 nm, 637 nm |
| Detection Channels | 8 | 15 | 15 | 27 |
| Plate Types | 96- and 384-well, 384- and 1536-well (Blue-Red HD) | 96- and 384-well | 96- and 384-well | 96-and 384- well |
| Min Sampling Time | < 5 minutes/96 wells, < 20 minutes/384 wells | |||
| Min Sample Aspiration | 1 µL | 1 µL | 1 µL | 1 µL |
| Detection Rate | Up to 35,000 events/second | |||
| Dynamic Range | > 7 Decades | 7.2 Decades | ||
| Clogging Detection | Bubble sensor to visually ID Clogs | |||
| Optional Products | Cat. No. |
|---|---|
| Forecyt® Enterprise Edition with 5 Floating Client Licenses | 91131 |
| Robotic Integration API License | 90208 |
| QMax Workstation | Request Info |
High-Throughput Screening (HTS) by Cytometry Applications
High-Throughput Screening (HTS) by Cytometry Applications to Support Your Most Important Research
To efficiently characterize disease states and to develop a clear picture of how single cells respond to a stimulus, whether it’s a small molecule, antibody, cytokine, or other entity, quantitative measurement of multiple biological readouts is required.
iQue® HTS by Cytometry Applications enable the assessment of multiple cell types and a range of cellular parameters in a single well, allowing for a streamlined high-throughput workflow, improved data coherence and a highly informed data set.
Request a Quote or Demo Explore All High-Throughput Screening (HTS) by Cytometry Applications
Antibody Characterization
Increase data throughput and quality by multiplexing antibody binding, function, and titer across the process.
Cytokine Profiling
Assess multiple cell parameters faster, with fewer cells and less reagents.
Cell Monitoring and Workflows
Streamlined combined workflows and protocols that are simple and easy to follow providing greater biological insights
Immune Cell Function
Understand the Complexities of the Immune System with the iQue® HTS Platform
Cell Health & Proliferation
Analyze cell health, viability and proliferation on a cell-by-cell basis with the iQue® HTS Platform
Related Products and Resources
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Additional Resources
iQue® High-Throughput Cytometry Publications
With over 1,200 references worldwide, the iQue®️ HTS Platform helps researchers explore new areas of study and address questions fundamental to life science research.
iQue® Cytometry Panel Builder
Use the dynamic, interactive panel design tool, for streamlining the creation of complex panels, to profile populations on different iQue® configurations..
