mAb Cell Line Development — Cell Banking
Cell Banking
Fully characterized, well-documented, homogeneous master cell banks (MCB) and working cell banks (WCB) are critical attributes to mitigate risk and ensure safety and product quality of any biological product.
Press Release: Sartorius Stedim Biotech Launches New Services for Mammalian Cell Bank Manufacturing — Read now
Intellicyt® iQue Screener PLUS
Measure and monitor bioprocess and Critical Quality Attributes (CQAs) for your antibodies, including apoptosis, autophagy, proliferation, and antibody binding.
MYCAP® CCX Cell Expansion System
Reliable aseptic technique for cell culture expansion, MYCAP® CCX has a revolutionary closed design ensuring media feed, inoculation, sampling, and transfers are done aseptically—without the need for a biosafety cabinet.
Sartoclear® Dynamics Lab P15 and Lab V
Rapid and convenient clarification of high-density mammalian cell cultures is often challenging. Sartoclear® Dynamics Lab provides a single-step clarification and sterile filtration protocol 70% faster than traditional centrifugation--without clogging. Quickly and easily prepare your clarified supernatant with your target molecule with our simplified, small-scale mammalian cell harvest tools for antibody preparation before further analysis.
Microsart® AMP Mycoplasma Kit
Mycoplasma are widespread contaminants in cell culture. Given their tiny size (~0.3-0.8 µm), mycoplasma cannot be visualized by microscopy. Cultivation-based detection takes 28 days, and even then, the colonies are difficult to visualize with the naked eye. Mycoplasma-contaminated products present a human health risk, so you must confirm your cell lines are uncontaminated.
Microsart® qPCR kits offer a safe and rapid method for early detection of mycoplasma contamination. It’s fast, highly specific and compliant with international guidelines.
BioOutsource Cell Bank Manufacturing
BioOutsource and Sartorius Stedim Biotech have combined their expertise to develop an industry-leading closed system, single-use and fully cGMP compliant cell banking manufacturing process with comprehensive PAT options available.
Resources
Application Note:
From Mammalian Cell Cultures to Pure Proteins: Sartoclear Dynamics® Lab Significantly Reduces Cell Harvest Time
Application Note:
Rapid Mammalian Cell Harvest without Centrifugation for Antibody Purification Using the Sartoclear Dynamics Lab Filtration System
Application Note:
The Mouse IgG Type and Titer Kit is a single, multiplexed assay that simultaneously quantifies IgG isotype, IgG quantity per isotype, total IgG secretion, cell count and cell health
Webinar:
Accelerating Nanobody Discovery Workflows with High-Throughput Flow Cytometry