Performance, Label-Free Biomolecular Interaction Analysis
For over 20 years, we have dependably supported our customers by creating established fluidic-free Bio-Layer Interferometry (BLI) platforms, consumables, and software. These products are trusted by scientists worldwide to support their bioanalytical needs for protein interaction analysis spanning from drug discovery to antibody engineering to bioprocessing and quality control (QC).
Our dedication to quality is reflected in an install base of more than 3,500 customers and is well-documented in more than 7,000 customer publications. Over the years, our top priority has been providing exceptional technical and scientific support, ensuring you can quickly and effectively utilize our products to achieve your experimental goals.
We continue to shape the future with Octet®, the only brand currently combining both industry-leading label-free technologies – Bio-Layer Interferometry and Surface Plasmon Resonance (SPR) for real-time label-free measurements of binding kinetics and affinity or to determine the active concentration of an analyte.
Unlock the true value of Octet® with these five fast facts:
Watch the Video: Octet® BLI Solutions
Performance, flexibility and confidence for real-time label-free, high-quality binding kinetics and analyte quantitation.
Octet®️ BLI systems offer an advanced, fast, robust, and fluidics-free approach for protein-protein and protein-small molecule analysis. The Octet®️ BLI platform allows direct detection of specific proteins or drug molecules - even in complex mixtures and unpurified samples such as cell culture supernatants and lysates.
Always opt for the genuine Octet® platform to empower your research by enabling faster time to market, quicker project completion and greater project capacity.
20 Years of BLI Expertise
Two Decades of Supporting Groundbreaking Scientific Research
Proven countless times – the genuine Octet® BLI is an industry-leading technology with more than 7000 peer reviewed publications covering a large variety of research areas and applications. The Octet® Publication Database provides an easy way to search scientific publications that reference and/or cite the use of our Octet® products for label-free biomolecular interaction analysis in real time.
Discover Our Literature Search Tool
3,500+ Octet® Instrument Installs Worldwide
We take pride in being a part of significant scientific breakthroughs worldwide. Our local teams of service engineers, technical support, and field application specialists are always available to provide you with the assistance you need, ensuring that you can continue to progress confidently in your research.
What's It Like to Be an Octet® User?
Break Free from Bargain Imitations — See What Genuine Octet® BLI Users Are Saying
Thousands of researchers worldwide use Octet® systems in diverse fields ranging from drug discovery to biopharmaceutical development and academic research to determine binding specificity, active analyte concentration, affinity, or binding kinetics in real-time. Discover what users of genuine Octet® BLI have to say about this indispensable tool that has helped them advance their research and accelerate the development of new therapeutics and diagnostics.
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Octet® Performance: Sensitive, Clean, and Reproducible Data
Baseline Stability, Low Noise, and Low Baseline Drift are Key for High-Quality Data
The Octet® R Series offers an advanced, fluidics-free approach to bimolecular analysis, with a wide variety of off-the-shelf biosensors for rapid binding kinetics and quantitation analysis. These systems utilize Sartorius’ genuine label-free Bio-Layer Interferometry (BLI) technology, enabling direct detection of specific proteins and other biomolecules — even in complex mixtures like crude cell culture supernatants and lysates.
The Octet® BLI systems monitor binding events in real time to calculate on rates (ka), off rates (kd) and affinity constants (KD). The superior sensitivity enables measurement of low molecular weight molecules down to 150 Da. The following example shows the kinetic interaction analysis of a small molecule.
Figure 1. The kinetic analysis of between a ligand, biotinylated-carbonic-anhydrase, and the small molecule analyte Furosemide (330.7 Da) with Super Streptavidin (SSA) Biosensors at 1000 rpm and 25°C on an Octet® R8 system. Furosemide working solutions were prepared in serial dilutions of 0.1, 0.3, 1.0. 3.0, 10.0 µM (n=4), respectively. Data was processed and curve fitted using a 1:1 binding model.
High Resolution and Low Noise to Measure High Affinity Interactions
Slow off-rate measurements show minimal changes in signals in the dissociation step requiring a combination of stable baselines and low baseline drifts for accurate measurement of the dissociation phase.
In addition, capturing measurable dissociation of the interaction may take hours of (> 2 hr data acquisition) specifically at the dissociation phase of higher affinity interactions that exhibit very slow off rates. Therefore, measuring longer dissociation times is also critical for measuring high-affinity interactions.
The Octet® R8 system simultaneously enables measurements of up to 8 samples in a single unattended run. Combined with a unique microplate cover that keeps samples intact by minimizing evaporation, it allows scientists to collect accurate measurements with evaporation control for up to 12 hours.
The measurement of one high-affinity interaction measured in triplicates in a long dissociation experiment on the Octet® R8 system is demonstrated below. Full kinetic characterization of an antibody-antigen interaction was run three times with a total run time of 11 hours.
Figures 2 and 3 provide two examples of long dissociation measurement over several hours to allow the detection of very slow off-rates.
Figure 2. A high-affinity interaction between an IgG and Fab measured in triplicates that required a long dissociation step time of more than three hours was measured using an Octet® R8 system. The total experiment run time was 11 hours for measurements made in triplicate.
Figure 3. Kinetics of high-affinity interaction between Human Epidermal Growth Factor (HER2) protein and anti-HER2 monoclonal antibody (Herceptin) analyzed in the Octet® R8 system demonstrating reliable high-resolution kinetic data.
Unprecedented Time and Cost Savings for Kinetics, Affinity, and Concentration Assays
Octet® BLI biosensors provide a fluidic-free design facilitating scalability in throughput and capability to assess interactions from crude, unpurified samples during early discovery, development, and manufacturing in real time for faster decision making.
Table 1: IgG antibody titer measurement using a conventional ELISA assay in comparison with a label-free Octet® BLI assay run on different systems to demonstrate the time-savings with running up to 96 samples in parallel.
BLI (Octet® R8) + Protein A biosensors | BLI (Octet® RH16) + | BLI (Octet® RH96) + | ELISA | |
|---|---|---|---|---|
Time to Results | <30 min per 96-well plate | <15 min per 96-well plate | 2 min per 96-well plate | 4 – 6 h per 96-well plate |
Operator Time | Approx. 10 min | Approx. 10 min robotics compatible | Approx. 10 min | 2 – 3 h |
Precision | < 5% | < 5% | < 5% | 15 - 20% |
Dynamic Range | 0.01 – 2000 µg/mL | 0.01 – 2000 µg/mL | 0.1 – 700 µg/mL | 1 – 100 ng/ml |
Sample Type | Crude cell culture supernatant (without centrifugation or filtration) | Crude cell culture supernatant (without centrifugation or filtration) | Crude cell culture supernatant (without centrifugation or filtration) | Cell culture supernatant |
Sample Preparation | None | None | None | Sample dilution 1:5,000 – 50,000 |
Assay Type | Real time | Real time | Real time | End point |
Octet® BLI Products are Confidently Adopted in Regulated Environments
Not Just GxP Compliance Tools — Rely on a Comprehensive Toolbox
From antibody quantitation, ligand binding characterization to potency assays, Sartorius offers a wide range of GxP compliance tools for developing validated assay methods on the genuine Octet® BLI platform.
The United States Pharmacopeia (USP) has recently released a new general chapter <1108> which for the first time cites the use of biolayer interferometry in key ligand binding applications that provide more precise kinetic data and that can measure a relatively wide range of binding affinities.
Explore Octet® Maintenance and Validation Services
Installation Qualification (IQ)
Verifies and documents all necessary components. Software required for operation are received and properly installed in accordance with Sartorius installation protocols.
Operational Qualification (OQ)
Tests critical subsystems of the instrument including optical system, shaker, and temperature controller to verify operating functions are in accordance with the Sartorius product operational specifications.
Performance Qualification (PQ)
Test performance of the system in the customer environment with pre-validated assays to verify instrument operating in accordance with a written and pre-approved specifications document by Sartorius.
Preventative Maintenance (PM)
The system lamps are replaced, calibrated, inspected for any potential issues, and proactively addressed. A comprehensive multipoint inspection and testing ensures each system meets the Sartorius product operational specifications.
Sartorius Offers Octet® 21 CFR Part 11 Software and a Full Line of GxP Products and Services as Part of the Octet® GxP Package Including Our Biosensor Validation Support Services
This service enables the Octet® BLI users to sample multiple lots of a biosensor during assay qualification and validation and reserve a well-characterized lot for purchase. Hence, customers can order up to five different biosensor lots, up to 20 trays from each lot for evaluation, and reserve up to 40 additional trays for future purchase.
Approved Drugs Utilizing Octet®️ BLI Data in Their Regulatory Approval Process
Octet®️ BLI assays can help identify potential safety concerns and improve the accuracy of drug evaluations. Here are several examples of companies, including Pfizer, AstraZeneca, or Roche, who have utilized such assays to generate data submitted as part of the supporting information for the drug’s approval with regulatory bodies such as the FDA or the EMA.
World-Class Octet® Customer Support
We Prioritize Providing Exceptional Customer Support to Ensure a Seamless Experience for Our Valued Customers
Our commitment to this is demonstrated through our:
- Live Global Technical Support — Live support is available via phone and email, ensures prompt responses within 24-48 hours.
- Knowledge Base — We understand the importance of troubleshooting and have developed the Octet® Knowledge Base, a comprehensive resource available anytime to assist users in resolving assay-related challenges.
- Software Access — Our customers can conveniently obtain the software they need, enhancing their experience with our products.
- Safety Datasheets & Certificates — Recognizing the significance of timely information, we have centralized Octet® safety datasheets and certificates of analysis, making them readily available online for immediate download.
Meet Some of Our Technical Support Team
Yixin Lin Ph.D, Manager, Global Technical Support
Helping Octet® BLI and SPR users for the past 13 years to navigate biosensor assay development, technical support, and troubleshooting and more than 20 years of experience in customer-facing environments. Yixin joined the Octet® team in 2010 as a Field Applications Scientist and is an expert in label-free biosensor assays, kinetics, immunoassays, biomarker proteomics, and statistics. Yixin leads a team of highly specialized scientists that provide world-class support to Octet® customers. Yixin enjoys taking walks, appreciating nature and city streets in her spare time.
Terence Hui, M.Sc. Manager, Field Applications Scientist
Terence is a dedicated hands-on protein biochemist and leads a highly skilled team of applications scientists in the Americas. He has been with the Octet® family for over 12 years and has been committed to advancing technical innovation, bringing extensive well-rounded experience in biological science, protein characterization, biosensor assay development, and people management. Outside of work Terence likes to explore new food, listens to music, and hiking.
Hendrik Wuensche, PhD, Technical Support Scientist
Hendrik has been supporting and guiding Octet® users in Europe to build SPR and BLI assays for more than 11 years. He is an expert in label-free biosensor assays, kinetics, quantitation, epitope binning, and drug discovery screening. Hendrik joined the Octet® team in 2012 as an Applications Scientist and has recently joined the technical support team supporting Octet® users globally. Hendrik likes mountain biking and caring for his bonsai trees in his spare time.
Tim Heiseler, Ph.D, Senior Application Scientist
Providing scientific customer support in the field of label-free interaction analysis for over 10 years across various European regions, Tim joined the Octet® team in 2013 as a Field Application Scientist. He has expertise in label-free interaction analysis, including kinetics and quantitation, as well as immunoassays, high-throughput screening, protein engineering and design. Tim is an integral member of the experienced BioA FAS EMEA team at Sartorius BioA, offering localized scientific support to their customers.
150 Years of Established Excellence
Our Company History
Sartorius was founded in 1870 as a precision mechanical workshop and has evolved into a leading international life science group. The Octet®️ business was integrated in 2020 to further the Sartorius mission of Simplifying Progress. For over 150 years we have helped researchers make scientific discoveries and we will continue to be here for you tomorrow.
