High Throughput System for Biologics Discovery, Development, QC, and Manufacturing
The Octet® R8 label-free protein analysis system provides fast, high throughput, and accurate characterization of biomolecules and is compatible with longer experimental run times and seamless integration into GxP-compliant labs during biologics development.
The 8-channel Octet® R8 system performs quantitation and kinetic analysis of up to 96 samples in 30 minutes to 2.5 hours, depending on the specific assay. An optional microplate evaporation cover minimizes losses in sample volume, allowing post-analysis sample recovery even after an experiment.
The Octet® R8 system can be used for a wide range of analyses including kinetic analysis, titer of IgG and other proteins, reagent qualification, immunoassay development, bioprocess development, quality analysis, crude antibody screening, epitope binning/mapping, ligand binding assays, small molecule analysis, elucidating cell signaling mechanisms, and infectious disease monitoring. Analysis can be done using a single channel or up to eight channels, enabling flexibility in sample throughput.
- Unmatched Flexibility and Versatility - Eight parallel, independent channels provide maximum speed, sensitivity, and flexibility
- Unattended Run Time – An optional evaporation cover facilitates up to 12 hours of unattended run time, while minimizing losses in sample volume and allowing post-analysis sample recovery even after an experiment
- Upgrade to GxP - The Octet® R8 GxP package is available with 21 CFR Part 11 software and IQOQPQ kits, protocols, and services for regulatory compliance. Choose when to upgrade a non-GxP system to a GxP system for regulatory compliance.
Explore the Fluidic-Free Industry Standard for Label-Free Analysis
The Octet® R8 Protein Analysis System Features
The Octet® R8 system monitors binding events in real time to calculate on rates (ka), off rates (kd), and affinity constants (KD).
The high sensitivity Octet® R8 system detects a diverse range of biomolecules from small molecules to proteins to cells.
The Octet® R8 system’s eight channels can be used independently to measure samples for screening purposes or in tandem, pairing the sample read with a dedicated reference for high-quality kinetic characterization.
Optional 21 CFR Part 11 software for Octet® R8 system available along with installation and qualification (IQOQ) and performance qualification (PQ) kits.
The sample plate temperature can be controlled from 15–40°C, enabling reliable kinetic determinations at different temperatures for temperature-sensitive proteins. Additional advantages of sample cooling include the ability to rapidly determine binding rate constants at multiple temperatures to extrapolate thermodynamic measurements. The Octet® R8 system’s eight channels can be used independently to measure samples for screening purposes or in tandem, pairing the sample read with a dedicated reference for high-quality kinetic characterization.
The Octet® platform provides faster time to results and process economy when compared to other technologies due to its fluidics-free design. Additionally, crude sample compatibility saves valuable time by eliminating the need for sample pre-treatment, and non-destructive testing conserves precious samples for other assays.
Applications
Kinetic and Affinity Characterization
The superior sensitivity of the Octet® R8 system enables measurement of both small and large molecules and kinetic constants over a broad range. The system accurately measure kinetic constants by bringing the detection surface directly to the sample, eliminating the need for microfluidics.
With ready to use biosensors for most proteins, the unique Dip and Read approach streamlines workflows and expedites assay development. It allows for direct measurement of crude samples with minimal instrument maintenance.
Protein Quantitation in Bioprocessing
Accurate and reproducible concentrations can be determined in as little as 2 minutes per sample or 32 minutes for a whole 96-well plate using a simple, one-step assay.
High sensitivity in quantitation can be easily achieved by increasing read time and shake speed, and even higher sensitivity can be achieved to sub-ng/mL levels with 2-step or 3-step assay formats, allowing automated measurement of contaminants such as host cell proteins and residual protein A, faster and more precisely than ELISA. Process economics can be improved further by regenerating and reusing the biosensors.
Quality Control (QC) and Manufacturing
The Octet® R8 system has been developed to operate reliably in regulated quality control (QC) environments. The Octet® is aptly used in method development and validation for lot release and in-process testing for ligand binding, concentration, and impurity analysis in both upstream and downstream processes.
The ease of use and simplicity in design together with 21 CFR Part 11 software and a full line of GxP products and services makes the Octet® R8 system an ideal analytical instrument for compliant labs.
Educational Resources
Product Resources
| Detection Technology | Bio-Layer Interferometry (BLI) |
| Biosensor Type | Disposable, single-use fiber optic biosensors with optional reuse by regeneration and/or re-racking in the biosensor tray |
Data Presentation | Graphs displaying real-time kinetic binding sensorgrams, fitted result plots, and residuals of fits Concentration data analysis including calibration curves and output of tabulated concentration data Tabulated kinetic data Epitope binning and cross-blocking matrices and trace overlays |
Sample Type | Proteins, antibodies, peptides, DNA, RNA, liposomes, viruses and VLPs in various media including serum, buffers containing DMSO, periplasmic fractions, untreated cell culture supernatants, and crude cell lysates |
Number of Spectrometers | 8 |
Maximum Simultaneous Reads | 8 |
Data Collection Rate | 2, 5 or 10 Hz |
Sample Position and Format | 1 standard 96-well, black, flat-bottom microplate |
Sample Volume | 180–220 µL/well Nondestructive testing |
Orbital Flow Capacity | Static or 100–1500 rpm |
Analysis Temperature Range | 15°C to 40°C, in 1°C increments |
Quantitation and Kinetics | |
Throughput | Up to 8 assays in parallel |
Molecular Weight Detection | >150 Da |
Analysis Time per Sample | Real-time kinetic binding experiments from 5 minutes to 4 hours, or up to 12 hours at 25°C with the evaporation cover |
| Association Rate Constant (ka) | 101 to 107 M-1 s-1 |
| Dissociation Rate Constant (kd) | 10-6 to 10-1 s-1 |
| Affinity Constant (KD) | 1 mM – 10 pM |
Baseline Noise (RMS) | <3.0 pm |
Baseline Drift | < 0.12 nm/hour |
Quantitation | |
Workflow | Up to 8 assays in parallel |
Analysis Time per Sample | Human IgG quantitation in 2 minutes for 8 samples, ≤ 32 minutes for 96 samples |
DirectQquantitation Range for Human IgG with Protein A Biosensor | 0.05 –2000 μg/mL |
Instrument | |
Weight | 72 lb (32.7 Kg) |
Dimensions (H x W x D) | 19.5 in x 22 in x 18.2 in (49 cm x 56 cm x 46 cm) |
