Designing and Measuring Drug Potency Using the Octet® BLI Ligand Binding Methods
Overview
Measuring drug potency is essential for ensuring the clinical effectiveness of therapeutic drugs throughout their lifecycle but designing accurate and reproducible potency assays can be challenging due to variability in biological assays.
This application note guides you through using the Octet® Bio-Layer Interferometry (BLI) platform to develop methods for determining EC50, a key measure of antibody-based drug potency. We describe two methods: a 2-step dose response and a 3-step dose response design, and provide case studies to illustrate each approach. With the Octet® software, you can easily compare EC50 values across multiple drug molecules, helping you assess the similarity between different molecules.
By reading this application note, you will enhance your understanding of ligand-based assays for surrogate potency assessment, ensuring quality and consistency in therapeutic drug products.
- Document type: Application Note
- Page count: 10
- Read time: 15 minutes
Key Takeaways
- Learn how to design and measure drug potency using Octet® BLI ligand binding methods
- Explore 2-step and 3-step dose response designs with practical case studies
- See how Octet® software supports robust methods development and high-throughput screening
- Benefit from reduced assay development time compared to traditional endpoint assays
