High Performance, Label-free Kinetics Characterization
Octet® R2 Protein Analysis System: Grows With Your Biomolecule Characterization Needs
The Octet® R2 System makes high-quality interaction analysis affordable and flexible. Priced for startup academic and biotech labs and low volume users are no longer bound by the trade-off between cost and performance when choosing a label-free assay system. The 2-channel Octet® R2 system offers an advanced, fluidics-free approach to interaction analysis, with a wide variety of off-the-shelf Dip and Read biosensors for binding kinetics and quantitation analysis. Octet® R2 provides a low-cost entry point for labs with lower throughput needs, while providing the flexibility to field-upgrade to 4 or 8 channels anytime the need arises.
The Octet® R2 System characterizes protein-protein and protein-small molecule binding interactions for early research and discovery. The multipurpose system also provides quantitative information by measuring active protein concentrations — even in complex mixtures like cell culture supernatants and lysates. With sensitivity for small molecule analysis, the Octet® R2 instrument enables the measurement of interactions between small molecules down to 150 Da in size, and their larger binding partners to determine accurate kinetics rates.
- Budget-Friendly - Designed for academic and startup labs. Low volume users are no longer bound by the trade-off between cost and performance when choosing a label-free assay system
- Flexible second channel for use as a reference or more samples - Unique 2-channel design has dedicated spectrometers for simultaneous referencing or added capacity.
- Grows With You – Decide later to upgrade to a 4-channel or 8-channel Octet® R- Series system for higher throughput. Upgrades are performed within a single visit by a field service engineer, without the hassle of trading in the old model
Key Features of the Octet R2 BLI System
The Benefits of the Octet® R2 Protein Analysis System
The Octet® R2 System monitors binding events in real- time measuring binding association (ka) and dissociation (kd) kinetics, and affinity constants (KD). The system’s two channels can be used independently for screening purposes, or in tandem, pairing the sample read with a dedicated reference for high-quality kinetic characterization.
Samples are loaded in standard microplates, eliminating fluidic line maintenance and expanding the number of samples that can be run in an assay. Pre-defined templates in Octet® BLI Discovery software streamline setup prior to running an assay and minimizes training needs. The software also offers users a range of parameters and metrics for analyzing acquired data in a powerful yet intuitive software interface.
The 2-channel Octet® R2 system directly detects the presence of specific proteins in solution with minimal interference from complex matrices. Accurate and reproducible concentrations can be determined in as little as 2 minutes per sample using a simple, one-step assay.
The sample plate temperature can be controlled from 15–40°C, which enables reliable kinetic determinations at different temperatures for temperature-sensitive proteins. Additional advantages of sample cooling include the ability to rapidly determine binding rate constants at multiple temperatures to extrapolate thermodynamic parameters.
The Octet® R2 system provides the ability to re-rack biosensors at the end of an experiment. Biosensor re-racking provides enhanced flexibility in loading ligands on biosensors and reduces operational costs when biosensors are loaded with precious target reagents.
Robust technology with simple, integrated user interface designed for biologists. Spend less time learning instrumentation and more time deriving insights.
Applications for the Octet® R2
Large Molecule Characterization
With pre-defined templates to follow in the Octet® BLI Discovery software, the Octet® R2 system streamlines setup prior to running an assay and minimizes training needs. Octet® Analysis Studio then gives users a range of parameters and metrics for analyzing acquired data, in a powerful yet intuitive software interface.
Small Molecule Characterization
With sensitivity to screen small molecule fragment libraries, the Octet® R2 instrument enables the measurement of interactions between small molecules down to 150 Da in size to determine accurate kinetics rates.
Educational Resources
Product Resources
| Detection Technology | Bio-Layer Interferometry (BLI) based on fiber optic biosensors |
| Biosensor Type | Disposable, single-use fiber optic biosensors with optional reuse by regeneration and/or re-racking in the biosensor tray |
Data Presentation |
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Sample Type | Proteins, antibodies, peptides, DNA, RNA, liposomes, viruses and virus-like particles in various media including serum, buffers containing DMSO, periplasmic fractions, untreated cell culture supernatants and crude cell lysates |
Number of Spectrometers | 2 |
Maximum Simultaneous Reads | 2 |
Data Collection Rate | 2, 5 or 10 Hz |
Sample Position and Format | 1 standard 96-well, black, flat-bottom microplate |
Sample Volume | 180–220 µL/well Nondestructive testing |
Orbital Flow Capacity | Static or 400–1500 rpm |
Analysis Temperature Range | 15°C to 40°C, in 1°C increments |
Quantitation and Kinetics | |
Throughput | Up to 2 assays in parallel |
Molecular Weight Detection | >150 Da |
Analysis Time per Sample | hIgG quantitation in 2 minutes for 2 samples Real-time kinetic binding experiments from 5 minutes to 4 hours for kinetic analysis |
| Association Rate Constant (ka) | 101 to 107 M-1 s-1 |
| Dissociation Rate Constant (kd) | 10-6 to 10-1 s-1 |
| Affinity Constant (KD) | 1 mM – 10 pM |
Quantitation Range | 0.5 µg/mL to 2000 µg/mL (Human IgG and Protein A Biosensor) |
Baseline Noise (RMS) | <3.0 pm |
Baseline Drift | < 0.1 nm/hour |
Quantitation Precision | CV <10% |
Instrument | |
Weight | 72 lb (32.7 Kg) |
Dimensions (H x W x D) | 19.5 in x 22 in x 18.2 in (49 cm x 56 cm x 46 cm) |
