Human Mesenchymal Stem Cell Culture 

Generate Pure High-Quality Human Mesenchymal Stem/Stromal Cells 

hMSCs are desirable tools in tissue engineering and cell therapy, but can be significantly affected by culture conditions and media components. Sartorius offers a completely defined, serum- and xeno-free system for hMSC isolation, expansion, and differentiation, including reagents for attachment, dissociation, and cryopreservation. MSC NutriStem® XF Medium supports superior hMSC proliferation and long-term growth with normal morphology, stable karyotype, and tri-lineage differentiation potential.

MSC NutriStem® XF Medium 

Find Your hMSCs Culture Solution

MSC NutriStem® XF supports the isolation and expansion of hMSCs, providing high-quality cultures for research and cell-based therapies. 

  • Xeno-free, serum-free  

  • Superior proliferation rate  

  • Maintain cell characteristics and differentiation potential 

  • Custom scale-up and optimization services 

  • Smooth transition from RnD to manufacturing

  • Full scientific and regulatory support 

  • Manufactured in accordance with applicable cGMP guidelines 

  • DMF available 

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Test MSC NutriStem® XF Medium

Sample media for stem cell-based therapies​​​​​​.

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Optimal Culture Media for Human Mesenchymal Stem/Stromal Cells

MSC NutriStem® XF media support superior growth and expansion of hMSCs derived from a variety of sources, including bone marrow (BM-MSC), adipose tissue (AT-MSC) and umbilical cord matrix (UC-MSC). 

  • Defined, xeno-free, serum-free formulations 

  • Support long-term growth of hMSCs  

  • Maintain hMSC self-renewal and multi-lineage differentiation potential 

  • Drug Master File (DMF) available 

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PLT Supplement for Human Mesenchymal Stem/Stromal Cell Culture

PLTGold® Human Platelet Lysate is a xeno-free, animal serum-free product derived from human platelets.

  • Contains all the growth factors and proteins necessary for hMSC growth 

  • Available in research and clinical grades 

  • Manufactured in a cGMP-compliant facility  

  • Does not require the addition of heparin 

  • Drug Master File (DMF) available 

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Optimal Adherence of Human Mesenchymal Stem/Stromal Cells

Attachment factors are critical for adherence of hMSCs, particularly when grown in serum-free culture.  

  • Xeno-free attachment solution  

  • Facilitate attachment, cell spreading, growth, morphology, differentiation, and motility of hMSCs

  • Manufactured in accordance with applicable cGMP guidelines

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Reliable Recombinant Trypsin for hMSC Dissociation

Recombinant Trypsin Solutions are animal component-free, defined cell dissociation enzymes that replace porcine trypsin and avoid animal-related variability and contamination.  

  • Ideal for dissociating attachment-dependent cells, such as hMSCs, in both serum-containing and serum-free conditions 

  • Can be directly substituted for trypsin without protocol changes 

  • Optimized for sensitive cell types, a critical characteristic in preventing harsh effects for downstream processing

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Efficient Differentiation of Human Mesenchymal Stem Cells

Whether for basic research, drug discovery, or therapeutic applications, stem cell differentiation requires standardized culture methods to ensure reproducible and reliable results. Sartorius offers a unique line of MSCgo™️ differentiation media.

  •  Serum-free and xeno-free

  • Effectively differentiate hMSCs into adipocytes, chondrocytes, and osteoblasts

  • Suitable for hMSC from various sources (hMSC-AT, hMSC-BM, and hMSC-CT)

  • Contains all the growth factors and supplements

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Superior Proliferation and Expansion of hMSCs

MSC NutriStem® XF Medium promotes superior proliferation and expansion of BM-hMSCs compared to other serum-free and serum-containing media. 

Long-Term Growth of hMSCs

MSC NutriStem® XF Medium promotes superior long-term growth of AT-hMSCs compared to other serum-free and serum-containing media.

High Expression of Mesenchymal Stem Cell Markers

Immunophenotyping results of AT-hMSCs at passage 2 using FACS analysis. hMSCs cultured in MSC NutriStem® XF Medium were found to be >99% positive for CD73, CD90, and CD105.

Differentiation Potential: Adipocytes, Osteocytes and Chondrocytes

Human AT-hMSCs were expanded in MSC NutriStem® XF Medium for 3-5 passages before differentiation. Representative images show stained adipocytes, osteocytes and chondrocytes.

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Benefits of MSC NutriStem® XF Media

Why Choose MSC NutriStem® XF Medium for Your hMSC Applications

Human mesenchymal stem/stromal cells are promising tools for a range of applications in both research and cell therapy. MSC NutriStem® XF Medium supports the culture of hMSCs in vitro for multiple passages while maintaining their immunomodulatory profile and multipotent differentiation potential, enabling the production of sufficient cell numbers required for many cell-based therapeutic strategies. 

  • Comprehensive portfolio — everything you need for optimal human mesenchymal stem/stromal cell culture 

  • Defined, serum-free, xeno-free or animal component-free media and reagents 

  • Scalable and customizable  

  • Manufactured in accordance with applicable cGMP guidelines

  • Regulatory and scientific support 

  • DMF available  

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FAQs - MSC Media

Human mesenchymal stem cells (hMSCs) are adult, multipotent stromal cells with the capacity to differentiate into mesodermal lineages including osteocytes, adipocytes, and chondrocytes, as well ectodermal (neurocytes) and endodermal lineages (hepatocytes). HMSCs are self-renewable (up to six passages), easily accessible, and cultures can expand in vitro with exceptional genomic stability. They have an adherent, fibroblast-like (spindle-cell phenotype), shoal-like pattern.

  • Positive markers: MSCs have a specific set of cell surface markers (CD73, D90, CD105).  HMSCs are non-hematopoietic.
  • Negative markers: MSCs lack expression of CD14, CD34, CD45, and human leucocyte antigen-DR (HLA-DR).

Mesenchymal stem cells (MSCs), also referred to as mesenchymal stromal cells, are adult stem cells capable of self-renewal and multilineage differentiation. MSCs can differentiate into cells of mesodermal lineages (adipocytes, chondrocytes, and osteoblasts) and non-mesodermal lineages (ectodermal and endodermal, including neuronal cells, cardiomyocytes, hepatocytes, and epithelial cells). The plasticity of MSCs confers benefits in tissue regeneration. MSCs can inhibit the immune system, promote cell survival, and induce angiogenesis among others pleiotropic activities. The immunosuppressive role of MSCs is particularly interesting for clinical use because it confers resistance to rejection by the host immune system after transplantation.

There are known protocols for isolating MSCs from different tissues. The protocols are well-published and the cells can usually be harvested in aesthetic clinics (e.g.,   during liposuction) and orthopedic wards. The stages include lysis of cells (mainly fibroblast), connective tissue digestion, pull down of MSCs, and seeding.

NutriStem® MSC XF Basal Medium is suitable to use from the earliest stage. The medium should be used after the lysis step and before seeding to wash the cells. The medium should be equilibrated at the incubator. The complete medium is used in the later stages of isolation, and in the proliferation and seeding of the MSCs. You can obtain MSCs from easily accessible sources using minimally invasive methods, and can rapidly expand them in large-scale clinical use for patient-specific, autologous therapies.

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Regulatory Considerations When Choosing Stem Cell Media

Explore the regulatory process and learn how to ease your path and overcome hurdles as you transition to clinical applications and scale-up.

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White Paper

Assuring the Multipotency of Your Human Mesenchymal Stem Cells

Our suggested protocol helps you ensure high quality and reproducibility when using hMSCs.

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Application Note

The Right Protocol and Medium to Isolate and Expand Your AT-MSCs

Isolate and expand your adipose-derived MSCs using our tested protocol and MSC NutriStem® XF Medium.

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Application Note

Passaging MSC Using NutriStem® XF Medium and Human Platelet Lysate

Combine MSC NutriStem® XF Medium with PLTGold® Human Platelet Lysate to support cell proliferation and culture longevity.

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MSC NutriStem® References

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