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Keep It Cool: Cellular Cryopreservation for Clinical Applications

Stem cells and T cells are critical raw materials for cell-based therapies, and usually, a large number of cells are required for clinical applications. Cryopreservation is crucial for these applications and is the only method to maintain the functional properties and genetic stability of cells for long-term storage.

However, the freeze-thaw process is stressful to the cells. The challenges with cryopreservation include low survival rate, altered gene expression and morphology, loss of cellular function, epigenetic changes, and differences in protein composition. Moreover, if optimal conditions are not provided during cell freezing, damage and cellular apoptosis or necrosis may occur post-thaw.

This webinar will explain how to overcome these challenges, describe the common methods for cryopreservation, offer suggestions for viability issues after cryopreservation, and provide tips for optimized and efficient cell cryopreservation that can facilitate translation to cellular therapy applications. In addition, the webinar will detail the considerations when choosing cryopreservation media and focus on the specific requirements necessary for keeping clinically accepted cells for cellular applications.

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What Will You Learn

  1. Summarize the common techniques and the associated challenges of cryopreservation.
  2. Explain how to maintain cell characteristics and obtain cell growth, high viability, and clinically accepted cells after cryopreservation.
  3. Identify tips and considerations for optimized and efficient cryopreservation.

Meet Our Expert

Oren Ben-Yosef, PhD

Product Manager for Stem Cells, Advanced Therapies

Oren has a BSc in Biotechnology Engineering and a PhD in Medical Sciences from the Technion Israel Institute of Technology, where his research focused on culturing methods and differentiation of embryonic stem cells and induced pluripotent stem cells. Oren joined Biological Industries, now part of Sartorius, in 2016, and he has been a Product Manager for Stem Cells since 2019.

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