Pluripotent Stem Cell Culture

Human Pluripotent Stem Cell Culture

Maintaining High-Quality Pluripotent Stem Cells

The transition from stem cell culture research models to clinical applications such as regenerative stem cell therapies require defined, high-quality culture systems and appropriate documentation. Sartorius provides an optimized cell culture environment for human induced pluripotent stem cells (hiPSCs) and human embryonic stem cells (hESCs), including the NutriStem®, serum-free, xeno-free media family, and its auxiliary reagents, manufactured in a cGMP-compliant facility.   

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NutriStem® hPSC XF Medium 

NutriStem® hPSC XF Medium is a powerful low-bFGF medium that works across research and clinical workflows, giving you ultimate control over your cultures. 

  • Defined, xeno-free, serum-free medium 

  • Designed for optimal growth and expansion of human iPSCs and hESCs 

  • Customizable formulation 

  • Custom scale-up services available 

  • Scientific and regulatory support 

  • Drug Master File (DMF) available 

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Test NutriStem® hPSC XF Medium

Sample media for stem cell-based therapies​​​​​​.

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Media Purchasing Made Easy

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Optimal Culture of hPSCs for Research and Cell Therapies

NutriStem® hPSC XF Medium supports superior growth and expansion of hiPSCs and hESCs.

  • Xeno-free and serum-free formulation 

  • Performance-validated over long-term culture 

  • Superb cell proliferation 

  • Excellent maintenance of pluripotency, normal karyotype, and gene expression over time 

  • Maximum flexibility — compatible with multiple matrices 

  • Drug Master File (DMF) available

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Optimal Medium for Reprogramming and Differentiation

NutriStem® hPSC growth factor (GF)-free contains no TGF or FGF, making it an ideal medium for many applications, such as reprogramming, embryoid body (EB) formation, and various differentiation assays. 

  • Flexible and compatible with multiple applications 

  • Facilitates transition of cells to or from complete NutriStem® hPSC culture medium 

  • Easily modified by the addition of chosen growth factors or cytokines to create a customized complete medium

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High Expression of Pluripotent Stem Cell Markers

H1 cells cultured in different media for 6 passages were analyzed using flow cytometry and gene expression. Cells cultured in NutriStem® hPSC XF Medium were >90% positive for SSEA-4 and Oct-4. 

Embryoid Body (EB) Formation

hESCs cultured in NutriStem® hPSC XF medium were tested for EBs at 14 days.
The 4 cell types found:  

A. Neural rosette
B. Neural rosette stained with tubulin 
C. Primitive blood vessels  
D. Megakaryocytes 

Easy and Reliable Single Cell Passaging

Recovery of H1 (61) hESCs from single-cell passage using NutriStem® hPSC XF and Recombinant Trypsin EDTA Solution on 0.5μg/cm² LaminStem™ 521. Colony morphology shown at 1 day and day 4 post-passage.

Healthy hPSC Colony Morphology

hiPSCs cultured in NutriStem® hPSC XF Medium on Matrigel™ display compact colonies and distinct colony morphology typical of hPSCs.

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NutriStem® hPSC XF Medium Scientific References

Find guides, references, and other resources for using and optimizing your NutriStem® hPSC XF Medium.

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Regulatory Considerations When Choosing Stem Cell Media

Explore the regulatory process and learn how to ease your path and overcome hurdles as you transition to clinical applications and scale-up.

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Improved Cellular Maintenance of Human Pluripotent Stem Cells

Seamlessly transfer to clinically compliant XF culture conditions to facilitate stable hPSCs that retain high levels of pluripotency.

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Complete Your Process With Comprehensive Solutions

Customized Media and Buffers

Robust and reliable production of customized cell culture media and buffers based on existing formulations or improvement of recipes.

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Cell Biopreservation Solutions

High-quality solutions designed and validated for the cryopreservation and cold storage of cells, including stem cells, T cells, and PBMCs.

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Ambr® 250 High Throughput

The Ambr® 250 system is a high throughput, automated bioreactor system for process development with 12 or 24 fully featured single-use 100 – 250 mL mi...

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Benefits of NutriStem® hPSC XF Media

Why Choose NutriStem® hPSC XF Medium for Your hPSC Applications

  • Achieve superior results and maximum control with the very first defined, xeno-free, serum-free medium for hPSCs.  

  • Maximum flexibility and compatibility — choose the matrix and passaging reagent that best suits your specific applications.  

  • Enhance with additional bFGF for your various culture needs 

  • Easily transfer to the clinic with complete regulatory support  

  • cGMP compliant 

  • Drug Master File (DMF) available 

  • Maintain the hPSC full differentiation potential and cell characteristics 

  • Customize for your unique needs — several packaging and volume fill options available including bags for bioprocessing 

  • Enjoy your weekend with a completely weekend-free protocol 

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FAQs - hPSC Media

Scientists use embryonic stem cells and induced pluripotent stem cells and their derivatives for applications like human development and disease mechanism studies, drug screening, toxicity testing, cell therapy, and organ regeneration. There are several known methods for somatic cells reprograming – the two most common are viral and non-viral RNA- and DNA-based vectors.

Pluripotent stem cells can undergo self-renewal and to differentiate into all cells of the tissues of the body. Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) fall under this definition.

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Application Note

Optimize and Scale Up Production of Human IPSC

This application note describes how Sartorius systems were used to create a scalable platform for iPSC production.

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Related Assets

White Paper

IPSC-Derived Cardiomyocytes

PDF | 389.2 KB
Application Note

Transition hPSCs From mTeSR™ 1 to NutriStem® Medium on Matrigel®

PDF | 888.0 KB
Application Note

Xeno-Free hiPS Cell Culture Using NutriStem® hPSC XF Medium

PDF | 1.7 MB
Application Note

Single Cell Passaging With NutriStem® hPSC XF on Laminin-521 Without the Need for ROCK Inhibitor

PDF | 1.8 MB
Application Note

Passaging Methods for hPSCs Under Feeder-Free Conditions

PDF | 897.2 KB
Application Note

Transition hPSCs From 8™ Medium to Nutristem® Medium on Matrigel®

PDF | 962.5 KB
Application Note

Transition hPSCs From 8™ Medium to Nutristem® Medium on Vitronectin

PDF | 731.9 KB
Application Note

Transition hPSCs From StemFlex™ to NutriStem® Medium on Matrigel®

PDF | 869.1 KB

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