Stem Cell Culture Media for Cell and Gene Therapy
Stem cell–based therapies hold tremendous potential to treat a wide range of conditions, including Type 1 diabetes, Alzheimer’s disease, and other applications in regenerative medicine. As these therapies continue to expand, the demand for high-quality stem cell media is rapidly increasing. Sartorius supports this need with animal component–free, xeno-free, and serum-free media solutions, along with a comprehensive portfolio of supplements, differentiation media, attachment factors, and dissociation solutions - helping you optimize every step of your workflow.
Designed to meet high quality standards, Sartorius solutions are extensively QC tested to support reliable performance in relevant pluripotent and multipotent stem cells.
Animal Component-Free, Xeno-Free, and Serum-Free Stem Cell Media Portfolio
Find the Right Stem Cell Media and Reagent for Your Application
Sartorius’ portfolio of stem cell culture media, reagents, and freezing solutions supports key stem cell workflows, including culture, reprogramming, differentiation, and cryopreservation. The culture media and supplements help maintain healthy stem cell growth, consistent cell performance, and lineage-specific differentiation to accelerate scientists’ progress in understanding disease mechanisms and developing advanced stem cell therapies.
| PSC Culture Animal-Component Free | PSC Culture Xeno-Free | MSC Culture | HSPC Culture | |
|---|---|---|---|---|
| Media | Nutri3D hiPSC ACF Medium | NutriStem® hPSC XF Culture Media | MSC NutriStem® XF Culture Media | CellGenix® GMP SCGM |
| Attachment | — | — | NutriCoat™ | — |
| Dissociation | Recombinant Trypsin Solution | Recombinant Trypsin Solution | Recombinant Trypsin Solution | — |
| Cryopreservation | NutriFreez® Cryopreservation | NutriFreez® Cryopreservation | NutriFreez® Cryopreservation | NutriFreez® Cryopreservation |
| Differentiation | — | NutriStem® hPSC XF GF-free as a basal medium | MSCgo™ Differentiation Media | — |
| Supplement | — | — | PLTGold® Platelet Lysate | — |
| View Products | View Products | View Products | View Products |
Human Induced Pluripotent Stem Cell (hiPSC) Culture
iPSCs are a powerful platform for patient-specific therapies, disease modeling, and predictive drug testing. Their use depends on culture media that preserve pluripotency for downstream differentiation. Nutri3D hiPSC ACF is an animal component-free medium for efficient and scalable hiPSC expansion from 2D seed train to 3D aggregate suspension culture, supporting high-density expansion and consistent cell quality.
hiPSC medium for reliable expansion and maintained pluripotency
Single-medium for 2D seed train to 3D scale-up
Animal component free and defined formulation
Manufactured under applicable cGMP guidelines
Promotes healthy 2D monolayers and 3D aggregates
DMF in process
Pluripotent Stem Cell (PSC) Culture
PSC culture conditions significantly impact cellular characteristics, growth, and potential downstream use. For therapeutic stem cell applications, cultures must be scalable and free of xenogenic factors. NutriStem® PSC media are derived from defined components and xeno-free.
Support long-term culture of PSCs
Preserve pluripotency and PSC normal karyotypes
Manufactured in accordance with applicable cGMP guidelines
DMF available
Mesenchymal Stem/Stromal Cell (MSC) Culture
MSCs are promising tools for regenerative medicine and cellular therapies. When expanding MSCs in vitro for translational use, a cGMP-compliant culture that generates large numbers of healthy, proliferative MSCs is vital. MSC NutriStem® is xeno-free and serum-free.
Exceptional MSC proliferation and rapid expansion
Used in multiple clinical trials worldwide
Manufactured in accordance with applicable cGMP guidelines
DMF available
Hematopoietic Stem and Progenitor Cell (HSPC) Culture
HSPCs are used for autologous and allogeneic cell therapies. CellGenix® GMP SCGM is an optimized, xeno-free medium used for the serum-free expansion of low numbers of human hematopoietic stem and progenitor cells.
- Serum-free cultivation of HSCs
- Expansion and differentiation of NK cells and CIK cells
- Manufactured under applicable cGMP guidelines
- FDA & PMDA DMF available
Ready to Explore Sartorius Stem Cell Solutions?
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Cell Culture Supplements
PLT Supplement for Human Mesenchymal Stem/Stromal Cell Culture
PLTGold® Human Platelet Lysate is a xeno-free, animal serum-free product derived from human platelets.
Contains all the growth factors and proteins necessary for hMSC growth
Available in research and clinical grades
Manufactured in a cGMP-compliant facility
Does not require the addition of heparin
Drug Master File (DMF) available
Optimal Adherence of Human Mesenchymal Stem/Stromal Cells
Attachment factors are critical for adherence of hMSCs, particularly when grown in serum-free culture.
Xeno-free attachment solution
Facilitate attachment, cell spreading, growth, morphology, differentiation, and motility of hMSCs
Manufactured in accordance with applicable cGMP guidelines
Reliable Recombinant Trypsin for hMSC Dissociation
Recombinant Trypsin Solutions are animal component-free, defined cell dissociation enzymes that replace porcine trypsin and avoid animal-related variability and contamination.
Ideal for dissociating attachment-dependent cells, such as hMSCs, in both serum-containing and serum-free conditions
Can be directly substituted for trypsin without protocol changes
Optimized for sensitive cell types, a critical characteristic in preventing harsh effects for downstream processing
Efficient Differentiation of Human Mesenchymal Stem Cells
Whether for basic research, drug discovery, or therapeutic applications, stem cell differentiation requires standardized culture methods to ensure reproducible and reliable results. Sartorius offers a unique line of MSCgo™️ differentiation media.
Serum-free and xeno-free
Effectively differentiate hMSCs into adipocytes, chondrocytes, and osteoblasts
Suitable for hMSC from various sources (hMSC-AT, hMSC-BM, and hMSC-CT)
Contains all the growth factors and supplements
Complete Your Process With Comprehensive Solutions
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FAQs - Stem Cell Media
Stem cells can differentiate to several cell types:
- Pluripotency stem cells are from embryonic cells or targeted cells that can be reprogrammed to iPSC. Both cell types have self-renewal properties, and can stay undifferentiated for at least 50 passages.
They can differentiate to each cell from the three lineages of the embryonic germ layer — endoderm, mesoderm, and ectoderm, and can be used to regenerate tissue in practice.
For these cells, scientists need a serum-free medium with controlled levels of growth factors to keep cells undifferentiated for as long as possible. As the serum is eliminated from the medium, scientists must add an attachment matrix or carrier (animal source or defined) to the culture to allow the cells to expand. - MSCs are multipotency cells from connective tissues of the body. They have the ability to stay undifferentiated for up to six passages, and can spontaneous differentiate to one of three cell types — chondrocytes, osteocytes, and adipocytes. The cells can be differentiated to other cell types by using specific growth factors. The media we offer keeps cells undifferentiated for at least six passages (depending on the cell source) and allows fast differentiation when needed. As serum is eliminated from the medium, you can add attachment factors or microcarriers.
Sartorius offers media for pluripotent stem cells (PSCs) and mesenchymal stromal cells (MSCs), as well as ancillary products for the culture process from isolation to expansion and differentiation finished with cryopreserved cells.
Media can keep PSCs undifferentiated for at least 50 passages and MSCs for at least 6 passages.
- PSCs: One ready-to-use bottle of NutriStem® hPSC XF Media.
- MSCs: Basal media with supplements. As the media is serum-free, it needs to be supplemented with attachment matrix. You can supplement PSCs with laminine and vitronectine, and MSCs with fibrinogen and fibronectin.
- Detachment: A peptide of recombinant trypsin with or without EDTA.
- Differentiation: Kits for MSC to chondrocytes, osteocytes and adipocytes.
- Cryopreservation: Medium based with 10% DMSO and salt based with 5% DMSO.
Once you have fully characterized the growth properties of your cells, a tailored media formulation can help optimize their growth and productivity.
You can get customized media formulations that add or remove elements, or create a complete custom cell culture media formulation to meet your specifications.
Totipotent stem cells: Totipotent (omnipotent) stem cells can differentiate into any of the 220 cell types found in an embryo as well as extra-embryonic cells (placenta).
Pluripotent stem cells: Pluripotent stem cells can differentiate into all cell types of the body (but not the placenta). Embryonic stem cells and induced pluripotent cells are under this potency.
Multipotent stem cells: Multipotent stem cells can develop into a limited number of cell types in a particular lineage. MSCs are under this potency.