Incucyte® CX3 Live-Cell Analysis System: Clarity from Complexity
The Incucyte® CX3 Live-Cell Analysis System empowers every scientist to study complex 3D cell models over time. Key to this capability is the new confocal imaging that produces clear images of organoids and spheroids. With exceptional flexibility, the CX3 supports simultaneous 2D and 3D assays, leverage fluorescence-based and label-free assays to continuously monitor morphology, growth, and phenotypic changes, gaining physiologically relevant data without disrupting cultures.
Complete with unmatched throughput and powerful software, the CX3 transforms 2D and 3D workflows, delivering clear insights that guide decisions in drug development, disease modeling, and personalized therapies.
Key Features:
- Choose between spinning disk confocal, wide-field fluorescence, HD phase, and brightfield imaging
- Multiplex with up to three fluorescence channels (Green, Orange, NIR)
- 4x, 10x, and 20x objectives on an automated turret
- Multi-user support with three interchangeable vessel trays, accommodating up to six microplates in parallel
- Continuous, long-term, non-invasive imaging of 2D and 3D models in a controlled environment.
See what the CX3 has to offer in Live-Cell Imaging & Analysis
More Colors. More Insights. More Possibilities.
Incucyte® CX3 Applications
The Incucyte® CX3 system offers excellent flexibility. Set up 2D and 3D cell assays simultaneously or perform comprehensive studies of spheroids and organoids using new confocal imaging. Choose both fluorescence-based and label-free assays to continuously monitor morphology, growth, and phenotypic changes in spheroids, organoids, stem-cell-derived models, and patient-derived 3D cultures, and gain physiologically relevant data without disrupting the culture. Additionally, the system is optimized for high-throughput workflows, making it ideal for large-scale applications such as:
- Compound screening and toxicity profiling in 3D tumor spheroids
- High-content assessment of drug response across organoid panels
- Parallel monitoring of differentiation and viability in stem-cell derived 3D models
- Time-resolved analysis of treatment effects in multi-well plate formats
Accelerate your next discovery with Incucyte’s suite of live-cell applications.
Cell Monitoring & Workflows
Imaging Vessels
The Incucyte® Live-Cell Analysis Systems are compatible with a wider range of culture vessels – currently over 700 and growing! From 6-well to 384-well microtiter plates to standard tissue culture flasks, the Incucyte® can support your live-cell imaging and analysis needs.
CX3 Specifications
| Category | Attributes | Description |
|---|---|---|
| Optical System | Fluorescence Channels | 3 |
| Optical Module | Green/Orange/NIR | |
| Objectives | 4x, 10x, 20x | |
| Objective automation | Automated turret | |
| Imaging Modes | Spinning Disk Confocal | |
| Widefield Fluorescence | ||
| Label-free HD Phase Imaging | ||
| Brightfield | ||
| Spinning Disk | 25 µm pinhole | |
| Multi-User Support | Remote Network Capability | |
| Unlimited, free user licenses | ||
| Independent vessel scheduling | ||
| Multi-objective scheduling | ||
| Incubation | Incubator size | >250L required |
| Incubation to 42ºC | ||
| Software Module Capability | Incucyte® Spheroid | |
| Incucyte® Organoid* | ||
| Incucyte® Cell-by-Cell | ||
| Incucyte® Advanced Label-Free | ||
| Incucyte® AI Cell Health | ||
| Incucyte® ScratchWound* | ||
| Incucyte® Chemotaxis* | ||
| Incucyte® Neurotrack* | ||
| Incucyte® Neuronal Activity* | ||
| Incucyte® 21 CFR Part 11* | ||
| Capacity and Vessel Compatibility | Interchangeable Trays | 3 |
| Microplate Capacity | 6 | |
| Microplate Compatibility | 6, 12, 24, 48, 96 and 384-well | |
| Flask Compatibility | T-25, T-75, T-100, T-175, T-225 | |
| Other Compatibility | Dishes and slides | |
| Computing and Storage | Operating System | Windows 11 loT Enterprise LTSC |
| Controller Storage | 43.6 TB | |
| Expandable RAID Storage | Add additional 87.2 TB with Incucyte® CX3 Data Storage | |
| Controller Memory | 64 GB |
Related Products
CX3 Resources
Featured Resources
What to Expect from our Virtual Demo
Because we want to provide the best and most relevant support to your research, Sartorius experts are available to demonstrate the features and benefits of our live-cell imaging and analysis systems in a personalized manner.
- Initial consultation with your local support team to understand your interests
- Virtual demo at no cost to you!
- Total demo time would be 2-4 hours, depending on number of applications
See how the Incucyte® can support your research needs, from setting up an assay to analyzing and exporting your data.
