Handbook: 7th Edition Live-Cell Imaging and Analysis

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7th Edition Live-Cell Imaging and Analysis Handbook — A Companion Guide for Incucyte® Users

Last updated: June 2026

Overview

The Live Cell Imaging and Analysis Handbook (7th Edition) is an industry leading resource for researchers seeking to advance discovery with real time cell analysis. 

This comprehensive guide explores how AI driven image analysis, label free workflows, and automated cell segmentation are transforming modern biology. 

Covering key applications in cancer research, immunology, and advanced 3D models such as spheroids and organoids, it highlights approaches for measuring proliferation, cell health, and kinetic responses with precision. 

Learn how to generate more predictive, data driven insights and unlock the full potential of live cell imaging in complex biological systems.  
  

  • Document type: Digital Book / PDF / 132 MB
  • Page count: 437
  • Read time: 12hr 00min

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Key Takeaways

  • A comprehensive and industry leading handbook covering real time, kinetic live cell imaging and analysis, with practical workflows across modern biological research
  • New chapter introducing advanced 3D live cell imaging using confocal technology to visualize and quantify spheroids, organoids, and complex cell models
  • New chapter on AI driven image analysis, highlighting automated segmentation, classification, and label free approaches for faster, more reliable data
  • Expanded content on label free and AI enabled analysis, including measurements of cell confluence, proliferation, morphology, and cell health
  • Dedicated sections on advanced cell models and applications, including cancer biology, immunology, stem cells, and co culture systems for translational research

Live-Cell Imaging and Analysis 7th Edition Handbook: Table of Contents

1. Introducing Real-Time Live-Cell Analysis


2. From Images to Answers


3. A New Dimension in Live-Cell Imaging


4. The Power of AI


5. Cell Culture Quality Control Assays

  • 5a. 2D Cell Culture Quality Control
  • 5b. 3D Cell Culture Quality Control
  • 5c. QC for advanced cell types


6. Kinetic Cell Health, Proliferation and Viability Assays

  • 6a. Kinetic Proliferation Assays
  • 6b. Kinetic Apoptosis Assays
  • 6c. Kinetic Cytotoxicity Assays
  • 6d. Kinetic Cell Cycle Assays
  • 6e. Kinetic Assays for Monitoring ATP and mitochondrial Integrity
      


7. Kinetic Assays for Studying Immune Cell Models

  • 7a. Kinetic Assays for Immune Cell Activation
  • 7b. Kinetic Assays for Immune Cell Killing
  • 7c. Kinetic Assays for NETosis
  • 7d. Kinetic Assays for Immune Cell Differentiation, Phagocytosis and Chemotaxis

8. Kinetic Cell Migration and Invasion Assays

  • 8a. Kinetic Scratch Wound Assays
  • 8b. Kinetic Chemotaxis Assays
  • 8c. Kinetic Transendothelial Migration Assays


9. Kinetic Assays for Quantifying Protein and Extracellular Vesicle Dynamics

  • 9a. Kinetic Antibody Internalization Assays
  • 9b. Kinetic Live-Cell Immunocytochemistry Assays
  • 9c. Kinetic Assays for EV uptake


10. Kinetic Assays for Utilizing Complex Models

  • 10a. Kinetic Multi-Spheroid Assays
  • 10b. Kinetic Single-Spheroid Assays
  • 10c. Kinetic Spheroid Invasion Assays
  • 10d. Embedded Organoid Assay

 

11. Kinetic Assays for Studying Neuronal Models

  • 11a. Kinetic Neurite Analysis Assays
  • 11b. Kinetic Neuronal Activity Assays
  • 11c. Kinetic Neuroimmune Assays 
      

12. Label-Free AI-Driven and Advanced Cell Analysis

  • 12a. Kinetic AI confluence Analysis
  • 12b. Kinetic AI Nuclei Analysis
  • 12c. Kinetic Cell-by-Cell Analysis
  • 12d. Kinetic AI Cell Health Analysis
  • 12e. Kinetic Advanced Label-Free Classification Analysis
  • 12f. Kinetic 3D Object-by-Object Analysis


13. Appendix