Live-Cell Imaging and Analysis System: Incucyte® S3

With the Incucyte® S3 Live-Cell Analysis System, take your research further with automatic acquisition and analysis of cells in a physiologically relevant environment. See more information in every sample and explore more applications - from cell health to complex functional assays - through utilization of up to two total fluorescence and HD phase imaging channels simultaneously. 

Key Features

  • Robust application suite
  • Multi-user, multi-application support through remote network capability and unlimited user licenses
  • Two-color Green/Red OR Orange/NIR fluorescence plus HD phase contrast imaging
  • 4X, 10X, 20X objectives on an automated turret
  • Support for three interchangeable vessel trays and over 700 vessels, compatible with flask, dishes, slides and microplates - up to six microplates in parallel.

Analyze even the most sensitive living cells around the clock for days, weeks or months. Never miss powerful insights again with the Incucyte® S3 Live-Cell Analysis System and associated reagents, consumables and software! 

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Explore the Incucyte® S3

Incucyte® S3 Features

See What Your Cells Are Doing and When They Do It with the Incucyte® S3

Protective Shield

Relevant Environment

Cells remain stationary while optics move below capture changes in biology, without disrupting cell cultures in the stable incubator environment

Product Hub

Remote, Networked Access

Interact with instrument at anytime from anywhere –  ideal for routine monitoring of assays and critical for restrictive laboratory conditions


Purpose-Built Software

Easy-to-use software to support all scientists across variety of cell models - turn complex assays into reliable and objective publication-ready data

Idea in Mind

More Insight

Accommodate multiple users and applications with up to six microplates, two different fluorescent channels and three objectives


Browse Publications

See how your peers are accelerating their studies with the latest Incucyte® research, featuring over 7000 references in peer-reviewed publications

Explore Our Publications

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Learn how the Incucyte® can support your research needs, from setting up an assay to analyzing and exporting your data with a virtual demo

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Key Features

Support for Multiple Users

  • Incucyte® accommodates up to six microplates at a time
  • Users can schedule experiments at different image acquisition frequencies and magnifications in parallel
  • Remote, networked access with unlimited, free licenses

Simple, Flexible Sample Prep

  • Compatible with a wide range of culture vessels and applications
  • Utilize Incucyte® reagents for minimal cell perturbance and maximum efficiency

Set Up & Walk Away

  • Brand new user interface for simple and flexible experimental set up
  • Track experimental conditions from setup through analysis

Acquire Images Over Days or Weeks

  • Leave your cells undisturbed in the incubator during image acquisition
  • Unique optical design – cells remain stationary while optics move
  • Multiple imaging modes – HD phase plus red and green fluorescence

View and Analyze in Real-Time...or Later

  • Efficient and reproducible image analysis
  • Powerful visualization of images and time courses

Incucyte® S3 Applications

Experience faster discovery with the suite of live-cell applications from Incucyte®. Obtain real-time insight into morphological and phenotypic changes for pathway and mechanistic studies by observing time-dependent and cell-dependent treatment effects. 

Begin your investigations from the moment cells are placed in culture.  Whether you work rare or expensive cell types such as primary cells, stem-cell derived cultures, patient derived cells, or recombinant cell lines, you can quantitate and validate cell morphology and phenotype at all stages of cell maintenance and modification. Furthermore, the applications enable analysis of a wide range of phenotypic cell-based assays.

With the insights gained from Incucyte® assays, you can make informed decisions, quickly optimize workflows, and efficiently study complex live-cell assays to accelerate your next discovery.


Download Incucyte® Applications Brochure

Imaging Vessels

The Incucyte® Live-Cell Analysis Systems are compatible with a wide range of culture vessels – currently over 700 and growing! From 6-well to 384-well microtiter plates to standard tissue culture flasks, the Incucyte® can support your live-cell imaging and analysis needs.  

Search Our Supported Vessels

S3 Specifications




Optical System

Fluorescence Channels


Optical Module 

Green/Red (Standard)

Orange/NIR (available for purchase)

Label-Free HD Phase Imaging


4X, 10X, 20X (all included)

Objective Automation 

Manual swap 

Multi-User Support

Remote Network Capability
Unlimited, Free user licenses
Independent Vessel Scheduling


Incubator Size 

>200 L required

Incubation to 42°C

Software Module Compatibility

Incucyte® Cell-by-Cell 
Incucyte® Spheroid
Incucyte® Cell Migration 
Incucyte® Chemotaxis 
Incucyte® Neurotrack 
Incucyte® Neuronal Activity* 
Incucyte® Angiogenesis 
Incucyte® AI Cell Health 

*Requires Orange/NIR Optical Module

Capacity and Vesselware Compatibility 

Interchangeable Trays


Microplate Capacity 


Microplate Compatibility 

6, 12, 24, 48, 96, 384-well

Flask Compatibility 

T-25, T-50, T-75, T-100, T-175, T-225

Other Labware Compatibility

35mm, 60mm, 100mm, 150mm dishes and chamber slides

Computing and Storage 

Operating System

64-bit Windows 10

Controller Data Storage

27.3 TB

Expandable RAID Storage

Add an additional 32.7 TB of storage with Incustore

Controller Memory

64 GB

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Discover which live-cell imaging and analysis instrument works best for your lab.

Learn More about the Incucyte® Instruments

Incucyte® S3 Resources

Featured Resources


Live-Cell Analysis 6th Edition Handbook

Real-time live-cell analysis is redefining the possibilities and workflows of cell biology. See how.

Download eBook

AI-Driven, Label-Free Quantification of Cell Viability

Learn about the benefits of AI-driven image analysis and label-free cytotoxicity assays

Watch the Webinar

Advances in Live-Cell Biological Image Analysis

This eBook explores biological live-cell imaging and analysis more deeply

Download eBook

Product Resources

Incuyte® Live-Cell Analysis S3 Technical Specifications

PDF | 654.6 KB

Incucyte® — Reagents, Consumables and Software

PDF | 8.8 MB
Incucyte - Empower Live-Cell Analysis Inside Your Incubator

Live-Cell Analysis Systems Brochure

PDF | 10.1 MB

FAQ for Live-Cell Imaging and Analysis Instrument: Incucyte® S3


The fluorescent channels vary depending on the instrument.  The Incucyte® S3 comes standard with two-color Green/Red fluorescence plus HD phase contrast imaging, but users have the option to separately purchase an Orange/NIR Optical Module for studies with sensitive cells that require imaging at longer wavelengths.

All Incucyte® models provide multi-user support with unlimited, free user licenses.  Additionally, multiple assays can be run and imaged in different channels in parallel.  However, only the Incucyte® SX5 and S3 support independent vessel scheduling for different image acquisition frequencies, and the Incucyte® SX5 is the only instrument equipped with an automated turret, providing multi-objective scheduling support.

The Incucyte® itself is not an incubator, but rather sits within a standard tissue culture incubator so cells are maintained in a physiologically relevant environment. For the Incucyte® S3, an incubator larger than 200L is recommended.

The Incucyte® S3 is a great instrument for your company. Incucyte® S3 shares the same highest maximum throughput as the Incucyte® SX5 but supports only the phase channel as well as green and red fluorescence.

What to Expect from our Virtual Demo  

Because we want to provide the best and most relevant support to your research, Sartorius experts are available to demonstrate the features and benefits of our live-cell imaging and analysis systems in a personalized manner. 

  • Initial consultation with your local support team to understand your interests
  • Virtual demo at no cost to you!
  • Total demo time would be 2-4 hours, depending on number of applications

See how the Incucyte® can support your research needs, from setting up an assay to analyzing and exporting your data.

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