Measure Phagocytosis in Real Time
The Incucyte® Live-Cell Analysis System enables real-time, automated phagocytosis assays within your cell culture incubator.
- Visualize and quantify phagocytosis using time-lapse imaging
- Automated analysis over the entire assay time course
- Simple mix and read 96/384-well protocols, no washing, no fixing, no lifting
- Highly sensitive measurements, low background and low cell numbers
Measuring Phagocytosis of Cells with Incucyte® Phagocytosis Assay
- Automatically quantify the phagocytosis of cells in real time
- Investigate efferocytosis and antibody mediated cellular phagocytosis (e.g., anti-CD47 / ADCP)
- Compatible with your choice of target cells labeled with the pHrodo® Cell Labeling Kit for Incucyte®
Measuring Phagocytosis of Bioparticles® with Incucyte® Phagocytosis Assay
- Monitor and measure uptake of bacteria or yeast pHrodo® Bioparticles® for Incucyte®
- Validate phagocytosis with images and movies
- Study the effect of treatments on full time course phagocytosis profiles
Incucyte® Phagocytosis Assays vs. Other Common Approaches
Common methods used to assess phagocytosis are often single point (e.g. High Content Analysis (HCA)), require cell lifting (e.g. flow cytometry) or washing/quenching of the labelled pathogen (e.g. fluorescein labelled).
Incucyte® | Plate reader | Flow cytometry | HCA | ELISA | |
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Real-time cell visualization |
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Integrated analysis |
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Mix-and-read, no wash or quench |
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Long-term kinetic measurements |
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Flexible choice of effector cells |
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No fixation/lifting |
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High sensitivity/low background |
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Low cell number/bioparticle number |
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