Tangential Flow Filtration Process Solutions

Sartorius is well known for its expertise in tangential flow filtration (TFF), providing advanced solutions that drive innovation in ultrafiltration and diafiltration (UF | DF) applications. TFF is widely used for the separation and purification of biomolecules, with versatile applications in microfiltration and UF | DF.

• Innovative Applications: TFF is traditionally employed for cell harvesting or perfusion processing via microfiltration, while ultrafiltration and diafiltration are particularly effective for concentrating and desalting proteins, nucleic acids, viral vectors, oligonucleotides, and other molecules derived from sources such as blood and plasma, vaccines, or other biopharmaceutical applications.
• Principle of Operation: TFF works by passing a solution across a membrane surface in a turbulent manner. Small particles pass through the membrane (permeate), while larger molecules are retained (retentate). Unlike classical dead-end filtration, target molecules remain in the retentate, allowing for recovery and concentration as the fluid volume is reduced through filtration.
• Optimized Performance: The turbulent flow across the membrane plays a crucial role in process performance. A gel layer forms at the membrane surface, and its thickness is influenced by flow rates and transmembrane pressure. These parameters must be optimized based on the specific application.
• Efficiency & Support: Ultrafiltration is typically used to concentrate the final product molecule, followed by buffer exchange, known as diafiltration. Sartorius is committed to supporting and optimizing these processes to ensure reliable and efficient TFF performance.