TFF Cassettes
Modern biopharmaceutical processes demand exceptional performance and efficiency. For tangential flow filtration (TFF), this means achieving high flux and ideal resolution capabilities to ensure efficient separation and purification, resulting in high product quantity and quality.
Sartorius tangential flow filtration cassettes provide high-performance separation, combined with full scalability and easy handling, across various applications.
TFF Cassettes Portfolio
TFF Membrane Materials Optimized for Your Needs
Sartorius offers two distinct membrane types, tailored to meet the diverse requirements of various applications.
Hydrosart®
The Hydrosart® membrane is a high-performance, stabilized cellulose-based membrane optimized for biopharmaceutical process applications.
Extremely hydrophilic, ensuring non-protein binding and virtually non-fouling properties.
Exceptional cleanability combined with high caustic stability, providing a long life cycle with stable performance in flux and retention.
Excellent chemical compatibility with commonly used solvents in ADC or oligonucleotide processing.
Polyethersulfone (PES)
The polyethersulfone membrane is well-established in the biotechnology and pharmaceutical industries.
High flux rates enable fast processing.
Stable polymer with a broad pH range (1-14) and temperature tolerance.
Minimal product loss due to low-adsorptive material.
Find Right TFF Filter Cassette for Your Needs
For ultrafiltration & diafiltration applications, e.g., proteins, monoclonal antibodies (mAbs), and virus particles.
| Attribute | Hydrosart® | Polyethersulfone (PES) |
|---|---|---|
Nominal Molecular Weight Cut-offs | 2, 5, 10, 30, 100, 300 kDa | 1, 5, 8, 10, 30, 50, 100, 300 kDa |
| Filtration Areas | 0.01 up to 14 m² | 0.01 up to 3.5 m² |
Typical Process Fit | Peptides, oligonucleotides, proteins, monoclonal antibodies (mAbs), vaccines, cell & gene therapy, pDNA, mRNA, ADCs, recombinant proteins, and viral vectors | Proteins (albumin), blood and plasma, monoclonal antibodies (mAbs), vaccines, cell & gene therapy, pDNA, mRNA, recombinant proteins, and viral vectors |
Self-Contained | Available | Partially available |
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For microfiltration applications (e.g. perfusion reactor or cell harvest).
| Attribute | Hydrosart® | Polyethersulfone (PES) |
|---|---|---|
Pore Size | 0.2 and 0.45 µm | 0.1 µm |
Filtration Areas | 0.2 up to 3 m² | 0.2 up to 3 m² |
Typical Process Fit | Cells and particles | Cells and particles |
| View Products | View Products |
Advantages of Single-Use TFF Cassettes
Single-use TFF cassettes significantly reduce setup and turnaround times compared to traditional reusable systems. By eliminating the need for cleaning and validation between batches, these cassettes streamline operations, enabling faster processing and rapid turnaround times.
The single-use design of these cassettes reduces cross-contamination risk and supports bioburden control by eliminating residual carryover between batches.
Single-use TFF cassettes are delivered pre-sterilized and in a ready-to-use format, supporting aseptic processing requirements. This reduces the need for additional sterilization steps and ensures compliance with stringent regulatory standards.
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Supporting Products & Services
Learn More About TFF Cassettes
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Need Optimization? Sartorius Expertise at Your Service
At Sartorius, we understand the complexities involved in evaluating and optimizing your TFF application. Our expertise can guide you through multiple aspects to ensure optimal performance:
- Choose the Right Cut-Off: Select a cut-off that is 2-3 times tighter than your target molecule size to ensure effective concentration.
- Evaluate Membrane Performance: Test different membranes to identify which offers the best performance for your specific application, considering factors like cleanability and caustic stability.
- TMP Scouting: Conduct trials with Sartorius support to determine the optimal transmembrane pressure (TMP) that balances flow rate and gel layer formation, ensuring efficient filtration.
- Operation Strategy Evaluation: Stability and efficiency can also be impacted by the final operation strategy. Besides TMP and flux-controlled strategies, pressure-controlled operations can provide even better performance within your application.
By systematically addressing these areas with Sartorius expertise, you can tailor your TFF setup to achieve optimal results.
Frequently Asked Questions
A crossflow filter is another term for a tangential flow filter cassette.
Ultrafiltration, also known as concentration, is used for concentrating molecules (e.g., monoclonal antibodies). Diafiltration refers to a buffer exchange, typically performed as a second step after the initial concentration step.
The optimal cut-off strongly depends on the final molecule size to be concentrated. Typically, a cut-off 2-3x tighter than the target molecule is recommended.
TMP stands for transmembrane pressure and is an important parameter during TFF optimization, along with the turbulence flow. The optimal conditions should be evaluated for every process and cassette type to achieve the best results.
