Hollow Fiber TFF

Our Hollow Fiber TFF Modules Enable Gentle and Fast Separation

Sartorius hollow fiber tangential flow filtration (TFF) modules provide high-performance separation in various upstream and downstream bioprocessing unit operations, including cell harvest, clarification, perfusion, diafiltration and concentration. They feature low binding modified polyethersulfone membranes (m-PES) that deliver consistently high process flux and product yields.

Available in three product lines, our hollow fiber (hollow fibre, HF) TFF modules have a small footprint and are easy to set up.

The CrossflowExpert™ calculators make it simple to determine the modules and operating conditions specific to your crossflow filtration application.

Watch this video to find out more about the time and cost benefits that Sartorius' singe-use Hollow Fiber filters can offer and how they can help to improve your processes.

Consult Our Experts


Modules are available in a range of batch volumes, membrane surface areas and module diameters to scale with your process.

Gentle Processing

A void-free flow channel geometry reduces shear stress and allows gentle separation of viruses, biomolecules and cells.

Filtration Flexibility

Our membranes feature various pore sizes for microfiltration and molecular weight cut-offs (MWCO) for ultrafiltration. Plus, we offer three different fiber IDs.

Cost and Time Savings

Choose self-contained, humectant free modules for a true single-use solution or opt for multi-use modules that are easy to clean and sanitize.

Expert Product Selection Assistance

Proprietary calculators (CrossflowExpert™) make it simple to determine the modules and operating conditions specific to your crossflow filtration application.

Hollow Fiber TFF Modules

Superior Filtration, Simple Setup

Sartorius hollow fiber (hollow fibre, HF) TFF modules are the ideal solution to save money, time and space. They come in a range of batch volumes, membrane surface areas and module diameters, which means they can provide a linear and predictive scale-up from laboratory to pilot-scale to manufacturing-scale by using matching materials, fluid-path length and performance characteristics.

Three different product lines are available to accommodate your specific process needs: the Single-use Line, the autoclavable Steamer Line, and the Re-use Line, which can be cleaned, stored and used repeatedly.


Single-use applications

Aseptic applications

Multi-use applications





Available in Assembly




Batch Volumes

10 mL up to 1500 L

10 mL up to 250 L

10 mL up to 1500 L

Pore Sizes &
Membrane Cut-offs

0.1 µm, 0.2 µm, 0.45 µm, 0.65 µm;
3 kD, 5 kD, 10 kD, 30 kD, 50 kD, 100 kD, 300 kD, 500 kD, 750 kD

Fiber ID

0.5 mm, 1.0 mm, 2.0 mm

Learn More

Learn More

Learn More


CrossflowExpert™ Calculators - Filtration Made Simple

CrossflowExpert™ calculators make it simple to determine what hollow fiber module and operating conditions are needed to perform a crossflow filtration application. Enter your data and the calculator will solve for the application.

  • Module Size
  • Shear Rate
  • Process Time
  • Water Flux

Configure Your Solution

Hollow Fiber TFF Modules

Single-use hollow fiber TFF (crossflow) modules offer a true ready-to-use separation solution. They are delivered humectant-free, gamma-irradiated, and fully integrity-tested. Since no cleaning is required, they save time and money.

  • Single-use solution
  • Ready-to-use
  • Small footprint  
  • Scalable design

The Steamer Line of hollow fiber TFF modules is designed for perfusion and aseptic applications. These modules are delivered gamma-irradiated and ready-to-use and can be incorporated into single-use or reuse systems.

  • Self-contained units
  • Autoclavable
  • Humectant-free — no pre-rinsing

The Re-use Line of hollow fiber TFF modules can be easily cleaned after each use — 20 times or more depending on the choice of cleaning solution, cleaning time and frequency, which is determined by the specific process stream, fouling tendency and operating conditions.

  • Self-contained — no assembly required
  • Rapid setup
  • Easy scalability

Single-use Assemblies

Customized Assemblies for Complete Single-use Separation

Our Single-use Line products allow you to design complete assemblies that provide all components needed for a fully integrated membrane separation solution. A customized Flow Assembly combines the Single-use Line hollow fiber TFF module of your choice with single-use pump heads; feed, retentate and permeate tubing; single-use pressure sensors; transducers; flow sensors and meters; and aseptic connectors, plugs or bags.

  • A range of batch volumes, from 10 mL up to 1500 L
  • Minimal facility footprint — horizontal or vertical setup
  • Gamma-irradiated to reduce the risk of cross-contamination
  • Pre-assembled and ready-to-use  
  • No cleaning validation required

Consult Our Experts

Purpose-Built Accessories

Accessories to Maximize the Value of Hollow Fiber TFF Modules

Consult with Sartorius experts to design a fully customized separation system incorporating our hollow fiber (hollow fibre, HF) TFF modules. Or build your own solution using our full portfolio of products and purpose-built accessories, which include monitoring and control systems, connector kits, and stands and holders.

Take advantage of vertical lab and production space with stainless steel Flow Stands and Flow Stand holders to secure your hollow fiber TFF modules. The Flow Stands come with easy-to-use handles to quickly adjust the height of your module or assembly.

  • Optimize placement of modules or assemblies on a lab bench or in a process system
  • Holders can be used interchangeably
  • Stands and holders sold separately

Flow Connector Kits are designed specifically to connect hollow fiber TFF modules to Flow Assemblies. The kits can include the following, as needed:

  • Clamps
  • Pinch valves
  • Reducers
  • Luer fittings
  • Silicone gaskets
  • Plugs
  • Caps
  • Single-use pressure sensors to monitor transmembrane pressure (TMP)

Consult Our Experts

Consult Our Experts

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