Hollow Fiber TFF
Our Hollow Fiber TFF Modules Enable Gentle and Fast Separation
Sartorius hollow fiber tangential flow filtration (TFF) modules provide high-performance separation in various upstream and downstream bioprocessing unit operations, including cell harvest, clarification, perfusion, diafiltration and concentration. They feature low binding modified polyethersulfone membranes (m-PES) that deliver consistently high process flux and product yields.
Available in three product lines, our hollow fiber (hollow fibre, HF) TFF modules have a small footprint and are easy to set up.
The CrossflowExpert™ calculators make it simple to determine the modules and operating conditions specific to your crossflow filtration application.
Modules are available in a range of batch volumes, membrane surface areas and module diameters to scale with your process.
A void-free flow channel geometry reduces shear stress and allows gentle separation of viruses, biomolecules and cells.
Our membranes feature various pore sizes for microfiltration and molecular weight cut-offs (MWCO) for ultrafiltration. Plus, we offer three different fiber IDs.
Choose self-contained, humectant free modules for a true single-use solution or opt for multi-use modules that are easy to clean and sanitize.
Proprietary calculators (CrossflowExpert™) make it simple to determine the modules and operating conditions specific to your crossflow filtration application.
Superior Filtration, Simple Setup
Sartorius hollow fiber (hollow fibre, HF) TFF modules are the ideal solution to save money, time and space. They come in a range of batch volumes, membrane surface areas and module diameters, which means they can provide a linear and predictive scale-up from laboratory to pilot-scale to manufacturing-scale by using matching materials, fluid-path length and performance characteristics.
Three different product lines are available to accommodate your specific process needs: the Green Line for single-use, the autoclavable Steamer Line, and the Reuse Line, which can be cleaned, stored and used repeatedly.
Perfusion and aseptic applications
Available in Assembly
10 mL up to 1500 L
10 mL up to 250 L
10 mL up to 1500 L
Pore Sizes &
0.1 µm, 0.2 µm, 0.45 µm;
0.5 mm, 1.0 mm and 2.0 mm
CrossflowExpert™ Calculators - Filtration Made Simple
CrossflowExpert™ calculators make it simple to determine what hollow fiber module and operating conditions are needed to perform a crossflow filtration application. Enter your data and the calculator will solve for the application.
- Module Size
- Shear Rate
- Process Time
- Water Flux
Hollow Fiber TFF Modules
Single-use hollow fiber TFF (crossflow) modules in our Green Line offer a true ready-to-use separation solution. They are delivered humectant-free, gamma-irradiated, and fully integrity-tested. Since no cleaning is required, they save time and money.
- Single-use solution
- Small footprint
- Scalable design
The Steamer Line of hollow fiber TFF modules is designed for perfusion and aseptic applications. These modules are delivered gamma-irradiated and ready-to-use and can be incorporated into single-use or reuse systems.
- Self-contained units
- Humectant-free — no pre-rinsing
The Reuse Line of hollow fiber TFF modules can be easily cleaned after each use — 20 times or more depending on the choice of cleaning solution, cleaning time and frequency, which is determined by the specific process stream, fouling tendency and operating conditions.
- Self-contained — no assembly required
- Rapid setup
- Easy scalability
Customized Assemblies for Complete Single-Use Separation
Our single-use Green Line products allow you to design complete assemblies that provide all components needed for a fully integrated membrane separation solution. A customized Flow Assembly combines the Green Line hollow fiber TFF module of your choice with single-use pump heads; feed, retentate and permeate tubing; single-use pressure sensors; transducers; flow sensors and meters; and aseptic connectors, plugs or bags.
- A range of batch volumes, from 10 mL up to 1500 L
- Minimal facility footprint — horizontal or vertical setup
- Gamma-irradiated to reduce the risk of cross-contamination
- Pre-assembled and ready-to-use
- No cleaning validation required
Accessories to Maximize the Value of Hollow Fiber TFF Modules
Consult with Sartorius experts to design a fully customized separation system incorporating our hollow fiber (hollow fibre, HF) TFF modules. Or build your own solution using our full portfolio of products and purpose-built accessories, which include monitoring and control systems, connector kits, and stands and holders.
Take advantage of vertical lab and production space with stainless steel Flow Stands and Flow Stand holders to secure your hollow fiber TFF modules. The Flow Stands come with easy-to-use handles to quickly adjust the height of your module or assembly.
- Optimize placement of modules or assemblies on a lab bench or in a process system
- Holders can be used interchangeably
- Stands and holders sold separately
Flow Connector Kits are designed specifically to connect hollow fiber TFF modules to Flow Assemblies. The kits can include the following, as needed:
- Pinch valves
- Luer fittings
- Silicone gaskets
- Single-use pressure sensors to monitor transmembrane pressure (TMP)