Octet HTX

96-Channel Ultra High Throughput Octet System: Octet HTX

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96-Channel Ultra High Throughput Octet System

  • Analyze up to 96 samples simultaneously - Perform your quantitation or kinetics experiments with unmatched speed. Quantitate up to 96 samples of an IgG sample in two minutes.
  • Perform a 32 x 32 epitope binning in less than eight hours - Use in-tandem, sandwich, or pre-mix assay designs to perform epitope binning. Complete a 32 x 32 epitope binning in less than eight hours.
  • Streamline workflows with automation – Automation-ready and robot-compatible for microplate and biosensor tray loading.

From IgG titer to epitope binning, the Octet HTX offers label-free molecular interaction analysts with state-of-the-art high throughput platforms that facilitate the rapid characterization and development of biologics drug molecules. 

Octet HTX
Simultaneously reads 8, 16, 32, 48 or 96 wells

The HTX read head provides design flexibility that allows users to analyze, 8, 16, 32, 48, or 96 samples at a time allowing for customized throughput up to 96 samples.

Full plate off-rate ranking in minutes, not hours

The Dip and Read assay format allows off-rate analysis of clones to be performed rapidly even in crude samples using ready-to-use biosensors off the shelf.

96-well plate quantitation in as little as two minutes

IgG titer determination of a full 96-well plate can be completed in two minutes. In addition, sample plate compartment is compatible with 384-well plates allowing for even more throughput.

32 x 32 epitope binning in less than eight hours

With the HTX, analysis of epitope binning matrices can be completed in record time, with 32 x 32 in less than eight hours. A powerful epitope binning analysis software enables easy visualization of data sets and provides a flexible data matrix that can accommodate a variety of cross-blocking formats.

Microfluidics-free Dip and Read format reduces assay time and maintenance cost

The Octet HTX utilizes Dip and Read™ technology with the read head dipping into samples in a plate format. With no fluidics involved, Octet systems are highly robust.

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Applications

ELISA Conversion

ELISAs are known for having variability in results, susceptibility to human error, and slow times-to-results. High throughput Octet systems are designed to overcome those limitations.  Convert your ELISA assay into an Octet assay.

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Epitope Binning

Epitope binning studies rely on the sequential binding of two antibodies to an antigen, and are performed using dozens of antibody pairs in cross-competition matrices. The Octet system excels at these large-scale studies due to assay speed, throughput, and exceptional reproducibility

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Protein Contaminant Testing

Protein contaminants are a major hurdle to overcome during the production of biopharmaceuticals. The Octet platform's rapid high throughput protein analysis combined with the anti-CHO HCP and Residual Protein A kits  provide biologics developers with a rapid and easy to use technique for contaminant testing.

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Featured Publications

Label-Free Detection Technologies: Key Considerations and Applications

Binding interactions in biological samples are dynamic and driven by changes to the environment. The techniques used to characterize these...

Read eBook

Automating Octet Assays for Ligand Screening

Drug development and production is challenging. Avitide, based in Lebanon, New Hampshire, provides on-demand development and supply of...

Read White Paper

Technology

Bio-Layer Interferometry (BLI) based on fiber optic biosensors

Sample volume

40–80 μL/well (384-well tilted bottom microplate) and 180–220 μL/well (96-well microplate), nondestructive testing, easily recovered

Data presentation

Sensorgrams displaying kinetic traces or concentration binding rates Epitope binning and cross-blocking matrices and trace overlays Tabulated kinetic or concentration data

Automation

Robot compatible microplate and biosensor tray loading

Orbital flow capacity

Static or 100–1500 rpm

Analysis temperature

Ambient + 4°C to 40°C, in 1°C increments

Association rate constant (ka)

101 to 107 M-1 s-1

Dissociation rate constant (kd)

10-6 to 10-1 s-1

Affinity constant (KD)

1 mM – 10 pM

Baseline noise (RMS)

<3 pm (8–16 biosensors); <8 pm (32–96 biosensors)

Baseline drift

< 0.1 nm/hour

Quantitation range

32–96 biosensors: 0.1–100 μg/mL of hIgG at 1000 rpm**; 1.0–700 μg/mL at 400 rpm 8–16 biosensors: 0.05–300 μg/mL of hIgG at 1000 rpm**; 0.5–2000 μg/mL at 400 rpm

Quantitation precision

CV <10%

Weight

200 lbs (90.7 kg)

Dimensions (H x W x D)

30.1 in x 31.5 in x 31.5 in (77 cm x 80 cm x 80 cm)

Sample type

Proteins, antibodies, peptides, serum containing media (up to 25%), DMSO containing buffers, virus-like particles, untreated cell culture supernatants and crude cell lysates

Sample plate

Standard, 96-well and 384-well, black, tilted bottom microplates

Molecular weight detection

>150 Da (8–16 biosensors), >5000 Da (32–96 biosensors)


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