96-Channel Ultra High Throughput Octet System
- Analyze up to 96 samples simultaneously - Perform your quantitation or kinetics experiments with unmatched speed. Quantitate up to 96 samples of an IgG sample in two minutes.
- Perform a 32 x 32 epitope binning in less than eight hours - Use in-tandem, sandwich, or pre-mix assay designs to perform epitope binning. Complete a 32 x 32 epitope binning in less than eight hours.
- Streamline workflows with automation – Automation-ready and robot-compatible for microplate and biosensor tray loading.
From IgG titer to epitope binning, the Octet HTX offers label-free molecular interaction analysts with state-of-the-art high throughput platforms that facilitate the rapid characterization and development of biologics drug molecules.
The HTX read head provides design flexibility that allows users to analyze, 8, 16, 32, 48, or 96 samples at a time allowing for customized throughput up to 96 samples.
The Dip and Read assay format allows off-rate analysis of clones to be performed rapidly even in crude samples using ready-to-use biosensors off the shelf.
IgG titer determination of a full 96-well plate can be completed in two minutes. In addition, sample plate compartment is compatible with 384-well plates allowing for even more throughput.
With the HTX, analysis of epitope binning matrices can be completed in record time, with 32 x 32 in less than eight hours. A powerful epitope binning analysis software enables easy visualization of data sets and provides a flexible data matrix that can accommodate a variety of cross-blocking formats.
The Octet HTX utilizes Dip and Read™ technology with the read head dipping into samples in a plate format. With no fluidics involved, Octet systems are highly robust.
Applications
ELISA Conversion
ELISAs are known for having variability in results, susceptibility to human error, and slow times-to-results. High throughput Octet systems are designed to overcome those limitations. Convert your ELISA assay into an Octet assay.
Epitope Binning
Epitope binning studies rely on the sequential binding of two antibodies to an antigen, and are performed using dozens of antibody pairs in cross-competition matrices. The Octet system excels at these large-scale studies due to assay speed, throughput, and exceptional reproducibility
Protein Contaminant Testing
Protein contaminants are a major hurdle to overcome during the production of biopharmaceuticals. The Octet platform's rapid high throughput protein analysis combined with the anti-CHO HCP and Residual Protein A kits provide biologics developers with a rapid and easy to use technique for contaminant testing.
Featured Publications
Technology | Bio-Layer Interferometry (BLI) based on fiber optic biosensors |
Sample volume | 40–80 μL/well (384-well tilted bottom microplate) and 180–220 μL/well (96-well microplate), nondestructive testing, easily recovered |
Data presentation | Sensorgrams displaying kinetic traces or concentration binding rates Epitope binning and cross-blocking matrices and trace overlays Tabulated kinetic or concentration data |
Automation | Robot compatible microplate and biosensor tray loading |
Orbital flow capacity | Static or 100–1500 rpm |
Analysis temperature | Ambient + 4°C to 40°C, in 1°C increments |
Association rate constant (ka) | 101 to 107 M-1 s-1 |
Dissociation rate constant (kd) | 10-6 to 10-1 s-1 |
Affinity constant (KD) | 1 mM – 10 pM |
Baseline noise (RMS) | <3 pm (8–16 biosensors); <8 pm (32–96 biosensors) |
Baseline drift | < 0.1 nm/hour |
Quantitation range | 32–96 biosensors: 0.1–100 μg/mL of hIgG at 1000 rpm**; 1.0–700 μg/mL at 400 rpm 8–16 biosensors: 0.05–300 μg/mL of hIgG at 1000 rpm**; 0.5–2000 μg/mL at 400 rpm |
Quantitation precision | CV <10% |
Weight | 200 lbs (90.7 kg) |
Dimensions (H x W x D) | 30.1 in x 31.5 in x 31.5 in (77 cm x 80 cm x 80 cm) |
Sample type | Proteins, antibodies, peptides, serum containing media (up to 25%), DMSO containing buffers, virus-like particles, untreated cell culture supernatants and crude cell lysates |
Sample plate | Standard, 96-well and 384-well, black, tilted bottom microplates |
Molecular weight detection | >150 Da (8–16 biosensors), >5000 Da (32–96 biosensors) |
- Brochure: Octet Systems PDF | 5.2 MB
- Selection Guide: Octet Biosensors PDF | 299.7 KB
- Datasheet: 384 Well Tilted Bottom Microplate PDF | 189.0 KB
- Datasheet: Octet CFR Software Validation Package PDF | 261.1 KB
- Datasheet: Octet Data Analysis HT Software Version 12 PDF | 649.8 KB
- Datasheet: Octet HTX Systems PDF | 1.7 MB
- Datasheet: Octet Software Comparisons PDF | 183.4 KB
- Datasheet: Octet BLI Discovery and Octet Analysis Studio Software Version 10.0+ PDF | 1.2 MB
- Datasheet: Octet System Qualification Products And Services PDF | 83.9 KB
- Datasheet: Sidekick Offline Biosensor Immobilization Station PDF | 222.7 KB
- Application Note: Enhancing Efficiency And Economics In Process Development And Manufacturing Of Biotherapeutics PDF | 543.3 KB
- Application Note: Converting ELISA Assay Into Octet Quantitation Assay PDF | 304.4 KB