High Throughput Platform for Biologics Development
From IgG titer to epitope binning, the Octet® RH16 system offers label-free biomolecular interaction analysis with state-of-the-art high throughput platforms that facilitate the rapid characterization and development of biologics drug molecules.
High detection sensitivity, throughput, and 384-microwell plate compatibility of the Octet® RH16 system have also made it a favorite for small molecule library screening and analysis of large cross-competition binding campaigns. The Octet® RH16 instrument also supports additional automation for extending walk-away times and reducing operator input. Target ligands can be batch immobilized onto biosensors and returned to the biosensor tray for re-use in future experiments, thus extending the number of uses for each biosensor, preserving valuable ligand, and maximizing flexibility in experimental design.
- Analyze up to 16 samples simultaneously
- Streamline workflows with automation - Automation ready and robot compatible for microplate and biosensor tray loading.
- GxP packages available - Available as complete packages with 21 CFR Part 11 software and IQOQ/PQ kits, protocols and services for regulatory compliance.
Arrange assay samples in 96- or 384-well plates depending on workflows and sample volumes
Supports larger sample and reagent capacities increasing walk-away times
The Octet® RH16 system utilizes Dip-and-Read™ technology where biosensors dip into samples housed in microplates. With no fluidics involved, Octet® systems have more uptime and lower operational costs
ELISAs are known for having variability in results, susceptibility to human error, and slow times-to-results. High throughput Octet® systems are designed to overcome those limitations. Convert your ELISA assay into an Octet® assay.
The epitope binning process identifies and arranges antibody candidates that bind similar epitope regions to an antigen into bins. Octet® epitope binning assays rely on testing the sequential binding ability of two antibodies to an antigen. Assay speed, throughput, exceptional reproducibility, and dedicated software tools make Octet® platforms ideal for analyzing large antibody libraries.
Protein Contaminant Testing
Protein contaminants are a major hurdle to overcome during the production of biopharmaceuticals. The Octet® platform's rapid high throughput protein analysis combined with the anti-CHO HCP and Residual Protein A kits provide biologics developers with a fast and easy-to-use assay for contaminant testing.
Bio-Layer Interferometry (BLI) based on fiber optic biosensors
40–80 μL/well (384-well tilted bottom microplate) and 180–220 μL/well (96-well microplate), nondestructive testing, easily recovered
Plots displaying kinetic binding, equation fits, and residuals of fits Tabulated kinetic data and data charts
Robot compatible microplate and biosensor tray loading
Orbital flow capacity
Static or 100–1500 rpm
Ambient + 4°C to 40°C, in 1°C increments
Association rate constant (ka)
101 to 107 M-1 s-1
Dissociation rate constant (kd)
10-6 to 10-1 s-1
Affinity constant (KD)
1 mM to 10 pM
Baseline noise (RMS)
≤ 4 pm (RMS)
< 0.1 nm/hour
8–16 biosensors: 0.05–300 μg/mL of hIgG at 1000 rpm**; 0.5–2000 μg/mL at 400 rpm
150 lb (68.2 Kg)
Dimensions (H x W x D)
30.1" H x 31.5" W x 31.5" D (77 cm H x 80 cm W x 80 cm D)
Proteins, antibodies, peptides, serum containing media (up to 25%), DMSO containing buffers, virus-like particles, untreated cell culture supernatants and crude cell lysates
Standard, 96-well and 384-well, black, tilted bottom microplates
Molecular weight detection
>150 Da (8–16 biosensors)