High Throughput Platform for Biologics Development
From IgG titer to epitope binning, the Octet® RH16 system offers label-free biomolecular interaction analysis with state-of-the-art high throughput platforms that facilitate the rapid characterization and development of biologics drug molecules.
High detection sensitivity, throughput, and 384-microwell plate compatibility of the Octet® RH16 system have also made it a favorite for small molecule library screening and analysis of large cross-competition binding campaigns. The Octet® RH16 instrument also supports additional automation for extending walk-away times and reducing operator input. Target ligands can be batch immobilized onto biosensors and returned to the biosensor tray for re-use in future experiments, thus extending the number of uses for each biosensor, preserving valuable ligand, and maximizing flexibility in experimental design.
- Analyze up to 16 samples simultaneously
- Streamline workflows with automation - Automation ready and robot compatible for microplate and biosensor tray loading.
- GxP packages available - Available as complete packages with 21 CFR Part 11 software and IQOQ/PQ kits, protocols and services for regulatory compliance.
Discover the Octet® RH16 & RH96 Systems
Features of the Octet® RH16
Arrange assay samples in 96- or 384-well plates depending on workflows and sample volumes
Supports larger sample and reagent capacities increasing walk-away times
The Octet® RH16 system utilizes Dip and Read technology where biosensors dip into samples housed in microplates. With no fluidics involved, Octet® systems have more uptime and lower operational costs
Applications
ELISA Conversion
ELISAs are known for having variability in results, susceptibility to human error, and slow times-to-results. High throughput Octet® systems are designed to overcome those limitations. Convert your ELISA assay into an Octet® assay.
Epitope Binning
The epitope binning process identifies and arranges antibody candidates that bind similar epitope regions to an antigen into bins. Octet® epitope binning assays rely on testing the sequential binding ability of two antibodies to an antigen. Assay speed, throughput, exceptional reproducibility, and dedicated software tools make Octet® platforms ideal for analyzing large antibody libraries.
Protein Contaminant Testing
Protein contaminants are a major hurdle to overcome during the production of biopharmaceuticals. The Octet® platform's rapid high throughput protein analysis combined with the anti-CHO HCP and Residual Protein A kits provide biologics developers with a fast and easy-to-use assay for contaminant testing.
Featured Publications
Technology | Bio-Layer Interferometry (BLI) based on fiber optic biosensors |
Sample volume | 40–80 μL/well (384-well tilted bottom microplate) and 180–220 μL/well (96-well microplate), nondestructive testing, easily recovered |
Data presentation | Plots displaying kinetic binding, equation fits, and residuals of fits Tabulated kinetic data and data charts |
Automation | Robot compatible microplate and biosensor tray loading |
Orbital flow capacity | Static or 100–1500 rpm |
Analysis temperature | Ambient + 4°C to 40°C, in 1°C increments |
Association rate constant (ka) | 101 to 107 M-1 s-1 |
Dissociation rate constant (kd) | 10-6 to 10-1 s-1 |
Affinity constant (KD) | 1 mM to 10 pM |
Baseline noise (RMS) | ≤ 4 pm (RMS) |
Baseline drift | < 0.1 nm/hour |
Quantitation range | 8–16 biosensors: 0.05–300 μg/mL of hIgG at 1000 rpm**; 0.5–2000 μg/mL at 400 rpm |
Quantitation precision | CV <10% |
Weight | 150 lb (68.2 Kg) |
Dimensions (H x W x D) | 30.1" H x 31.5" W x 31.5" D (77 cm H x 80 cm W x 80 cm D) |
Sample type | Proteins, antibodies, peptides, serum containing media (up to 25%), DMSO containing buffers, virus-like particles, untreated cell culture supernatants and crude cell lysates |
Sample plate | Standard, 96-well and 384-well, black, tilted bottom microplates |
Molecular weight detection | >150 Da (8–16 biosensors) |
