Bio-Layer Interferometry (BLI) Label-Free Technology

What is Bio-Layer Interferometry (BLI) Label-Free Technology?

Bio-Layer Interferometry (BLI) is a label-free technology for measuring biomolecular interactions. It is an optical analytical technique that analyzes the interference pattern of white light reflected from two surfaces: a layer of immobilized protein on the biosensor tip, and an internal reference layer.

Any change in the number of molecules bound to the biosensor tip causes a shift in the interference pattern that can be measured in real-time.

The binding between a ligand immobilized on the biosensor tip surface and an analyte in solution produces an increase in optical thickness at the biosensor tip, which results in a wavelength shift, which is a direct measure of the change in thickness of the biological layer. Interactions are measured in real time, providing the ability to monitor binding specificity, rates of association and dissociation, or concentration, with precision and accuracy.

Only molecules binding to or dissociating from the biosensor can shift the interference pattern and generate a response profile on the Octet® System. Unbound molecules, changes in the refractive index of the surrounding medium, or changes in flow rate do not affect the interference pattern. This is a unique characteristic of BLI and extends its capability to perform in crude samples used in applications for protein binding, quantitation, affinity, and kinetics.

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Resources for BLI Technology

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Octet® BLI Systems

Unmatched Versatility for Discovery, Development and Quality Control

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Octet top 10 reasons to choose BLI

Sartorius Octet® BLI Technology

Top Ten Reasons to Choose Sartorius Octet® BLI Technology to Accelerate Your Research, Development and Manufacturing

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eBook: How Are Label-Free Technologies Used in Early Drug Discovery?

How Are Label-Free Technologies Used in Early Drug Discovery?

Modern analytical techniques designed for real-time analysis and high-throughput capabilities significantly reduce the time to the discovery

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Influenza Vaccine Titre Determination Bio-Layer Interferometry (BLI)

Fast, accurate determination of vaccine titre during influenza vaccine manufacture is important in understanding process performance and correctly sca...

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Application Note

Biomolecular Binding Kinetics Assays on the Octet® Platform

Introducing label-free platforms, the technology, capabilities, and advantages of label-free binding assessment

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Label-Free Detection technologies, key considerations and applications cover

Label-Free Detection

Technologies, Key Considerations, and Applications

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