High-Performance Label-free Analysis: Fast, Sensitive, Scalable
The Octet® R8e BLI System is the latest innovation in label-free, real-time biomolecular analysis, offering the most advanced detection capabilities in biolayer interferometry (BLI) technology.
Designed to support complex workflows in drug discovery, process development, and GxP environments, the Octet® R8e delivers superior sensitivity, flexible assay design, and longer run times.
Complete with 21 CFR Part 11 compliance solutions, this next-level BLI system facilitates straightforward and confident data-driven decision-making.
Introducing the Octet® R8e
- Best-in-BLI Sensitivity: Improved signal-to-noise expands assay range to low-molecular-weight and low-concentration analytes.
- Flexible Throughput: Compatibility with 96-well and 384-well microplates allows sample volumes as low as 40 μL.
- Extended Walk-Away Operation: New evaporation control protects sample. quality during long runtimes of up to 16 hours.
- Industry-Level Support: Easy assay transferability across the platform, along with 21 CFR Part 11 compliance options, ensures adherence to regulatory standards.
Key Features of the Octet® R8e
See what the Octet® R8e BLI System has to offer in label-free biomolecular interaction analysis.
Sensitivity
Best-in-class sensitivity with higher signal-to-noise ratio allows for precise detection of low molecular weight and low concentration analytes
Data Acquisition
Enhanced data acquisition captures crucial data points for reliable kinetics determination, even at the fastest speeds
Evaporation Control
Maintains analyte concentrations for up to 16 hours, enabling accurate data generation during extended and overnight use
Flexibility
Adaptability with 96- or 384-well plate formats, both standard and tilted, allows for flexible experimental throughput and sample requirements tailored to your needs
Fluidic-Free Design
Unprecedented time and cost savings owing to the simplified setup and operation of the system
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See the Octet® R8e in action and discover how it can transform your research
Explore the Octet® R8e Protein Analysis System
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| Detection Technology | Biolayer Interferometry (BLI) |
| Biosensor Type | Disposable, single-use fiber optic biosensors with optional reuse by regeneration and re-racking in the sensor tray |
| Information Provided | Yes/No binding - Kinetic and affinity analysis (kobs, ka, kd, KD) - Specific and selective detection of molecules, even in crude samples - Relative and absolute quantitation of specific proteins in crude matrices or purified samples - Relative potency (EC50) and Inhibition (IC50) analysis? |
| Sample Types | Proteins, antibodies, peptides, DNA, RNA, liposomes, viruses and VLPs in various media including serum, buffers containing DMSO, periplasmic fractions, bacterial cells, nanoparticles, untreated cell culture supernatants and crude cell lysates |
| Number of Spectrometers | 8 |
| Maximum Simultaneous Reads | 8 |
| Data Collection Rate | Variable |
| Sample Position and Format | One standard 96-well or 384-well black, flat bottom microplate, or 384-well black tilted well plate |
| Minimum Sample Volume | 40 µL |
| Orbital Flow Capacity | Static or 100–1500 rpm |
| Analysis Temperature Range | 15–40°C |
Kinetics | |
| Workflow | Up to 8 assays in parallel |
| Molecular Weight Detection | >100 Da |
| Association Rate Constant (ka) | 101 to 107 M-1 s-1 |
| Dissociation Rate Constant (kd) | 10-6 – 0.1 s-1 |
| Affinity Constant (KD) | 1 mM – 10 pM |
| Baseline Noise (RMS) | < 0.001 nm |
| Baseline Drift | < 0.1 nm/hour |
Quantitation | |
| Workflow | Up to 8 assays in parallel |
| Direct quantitation range for ProA biosensor | 0.01 - 4000 μg/mL |
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Frequently Asked Questions
The Octet® R8e features advanced evaporation control for the 96-well plate format, allowing for up to 16 hours of uninterrupted operation while maintaining analyte integrity, reducing the need for manual intervention, and improving data accuracy. This means the system delivers consistent, reliable results even during long or over-night experiments.
The Octet® R8e System is a cutting-edge, 8-channel biolayer interferometry (BLI) system designed for real-time, label-free biomolecular analysis. It features a fluidic-free design that minimizes maintenance, simplifies workflows, and ensures consistent data accuracy. The Octet® R8e boasts best-in-class sensitivity, extended walk-away operation, and supports GxP compliance. With its high throughput and robust performance, the system is ideal for complex drug discovery and process development applications.
With compatibility for both 96- and 384-well plates, the Octet® R8e is designed to support high-throughput workflows. The system’s 8 channels enable parallel data acquisition, allowing labs to process multiple samples simultaneously, boosting efficiency and maximizing productivity.
Yes, the Octet® R8e is designed for seamless assay transferability across platforms. Methods developed on older Octet® BLI systems can be easily transferred without compromising data integrity or comparability, ensuring smooth transitions and consistent results across your lab’s operations.
The Octet® R8e requires as little as 40 µL of sample per well, making it highly efficient and cost-effective, especially when working with precious or limited sample quantities. This low sample volume capability helps conserve reagents and minimize costs. Furthermore, the measurement is non-destructive and the sample plate can be recovered for further usage.
