Affinity chromatography offers a highly specific interaction between a target molecule and its ligand. This interaction reduces major contaminants to a low level within a single step. Sartorius offers multiple affinity chromatography matrices with high binding capacities, in a variety of selectivities to purify recombinant proteins, albumin, enzymes coagulation factors (ATIII), plasminogen, growth hormones, monoclonal antibodies, and mRNA.
Choose the Right Affinity Matrix for Your Application
Chromatography matrices are not one-size-fits-all. Choosing the matrix best suited for your application will ensure streamlined process development, scale-up, and manufacturing without sacrificing purification performance.
Find the Right Solution for Your Affinity Chromatography Process
Sartorius offers multiple affinity chromatography resins with high binding capacities in a variety of selectivities to purify recombinant proteins, albumin, enzymes coagulation factors (ATIII), plasminogen and growth hormones.
Coagulation factors (ATIII), growth hormones and enzyme purification
Plasminogen, recombinant protein and glycoprotein purification
Albumin, enzymes, recombinant proteins and purification
Dynamic binding capacity (10 % BT)
> 25 mg/mL
> 10 mg/ml
Average particle size
Working pH range
1 – 10
<3 barg (44 psig)
<3 barg (44 psig)
Heparin HyperD® M resin is suited for the purification of heparin-binding proteins like coagulation factors, growth hormones, lipoproteins, and DNA/RNA processing enzymes.
Lysine HyperD® affinity resin is used for the purification of lysine-binding proteins such as plasminogen. It provides high protein-binding capacities at high flow rates.
Coming Soon: A Built-for-Purpose Membrane for Rapid Cycling Chromatography (RCC)
Rapid Cycling Chromatography in bioprocessing offers extremely high productivity compared to traditional and multi-column chromatography.
Implementing RCC requires the fine-tuned interaction of the appropriate devices, a supporting skid technology, and a purpose-designed membrane to provide high mass transfer combined with high permeability.
In addition to 10 to 15 times higher productivity, RCC allows process simplification, miniaturization and flexibility, bioburden risk mitigation and bound capital reduction.
Highly Specific Monolithic Affinity Purification
Monolithic columns combined with unique affinity ligands offer an unmatched solution for affinity purification of large biomolecules.
- Channel sizes: 2 µm
- Column volume: 1 mL – 8 L
- Working range, pH: 2 - 13
- Functionality: Thymine oligomer based affinity
- Application: mRNA
CIMmultus® Oligo dT – Affinity for RNA
Oligo dT ligands covalently bound to solid support
Hybridizes to poly-adenylated tail found on most eukaryotic mRNAs, or synthetized onto the molecule during IVT
Fast and efficient capture and purification of mRNA with a poly-adenylated tail, from various sources
Selectively removes impurities from samples
Convenient approach for initial purification of mRNA