ion exchange chromatography molecules

High-Capacity, High-Selectivity Matrices For Efficient Processes

Ion Exchange Chromatography

Ion exchange matrices are the first step in capture and polishing chromatography of various biomolecules. Satorius offers a comprehensive platform of resins, chromatography membranes, and monoliths to match nearly any application:

  • Resins exhibit ideal binding capacities for small and medium-size proteins.
  • Membranes and monoliths have a higher binding capacity for viruses, virus-like particles, and other large complex molecules while also delivering higher productivity thanks to reduced processing times.
  • Unique salt-tolerant matrices like HyperCel™ Star AX anion exchange resin and Sartobind® STIC membrane provide simple process integration without additional feedstream manipulation.
Flexible processing

Salt-tolerant matrices reduce or eliminate dilution or adjustment steps
 

High productivity

Plug-and-play chromatography matrices allow high-speed processing without sacrificing capacity


Industry proven

Ion exchange matrices are widely established in various commercial processes


The Right Solution for Your Ion Exchange Chromatography Process

Ion exchange matrices play a key role in bioprocessing for capture and purification steps. While many ion exchange matrices are available, selecting the suitable matrix for your application ensures streamlined process development, scale-up, and commercialization.

Whether you are searching for high capacity or are working with large molecules, Sartorius has an ion exchange matrix to fit your purification needs.


Monoliths

Membranes

Resins

Application 
 

Capture and purification of large biomolecules

Capture of large biomolecules, flow-through purification of all biomolecules. Complementary solution to monoliths for large biomolecule purification

Capture and purification of small and medium biomolecules

Implementation 

Intrabatch multi-use, single-use between batches

Intrabatch multi-use, single-use between batches

Multi-use

Ease of use 

Ready-to-use

Ready-to-use

Bulk material (simplified packing)

Scalability 

Wide range of device sizes

Capsules and cassettes for linear scale-up

Wide range of column sizes 

Learn More

Learn More

Learn More

Monoliths for IEX Chromatography

IEX Monolithic Chromatography

Benefit from all the attributes of monolithic chromatography with a range of strong and weak anion and cation exchangers to meet the needs for purification of large biomolecules.
 

CIMmultus® SO3

CIMmultus® QA

CIMmultus®
DEAE

CIMmultus® EDA

CIMmultus® EV

Channel sizes 

1.3 | 2 | 6 µm 

1.3 | 2 | 6 µm

1.3 | 2 | 6 µm

2 µm

2 µm

Column volume 

1 mL – 8 L

1 mL – 8 L

1 mL –8 L

1 mL – 8 L

1 mL – 8 L

Working range, pH 

2 – 13

2 – 13

2 – 9

2 – 9

2 – 13

Functionality 


Strong cation exchange 

Strong anion exchange 

Weak anion exchange 

Weak anion exchange 

Strong anion exchange 

Typical application 


Viruses, (i.e. AAV),
and VLPs

Viruses, (i.e. AAV),
and VLPs

pDNA

pDNA

Exosomes

Request a Quote

Request a Quote

Request a Quote

Request a Quote

Request a Quote

Learn More

Learn More

Learn More

Learn More

Learn More

CIMmultus® SO3 – Strong CEX

  • Strong cation exchanger

  • Working range, pH 2–13

  • Binds molecules with predominantly positive charge, repels molecules with predominantly negative charge

  • Capture, intermediate, or polishing steps in either bind-elute or flow-through mode

  • High capacity for purification of virus and virus-like particles

  • Sanitizable with 1 M NaOH

Request a Quote

CIMmultus® QA – Strong AEX

  • Strong anion exchanger 

  • Working range, pH 2–13 

  • Binds molecules with predominantly negative charge, repels molecules with predominantly positive charge 

  • Capture, intermediate, or polishing steps in either bind-elute or flow-through mode 

  • High capacity for virus particles, IgM, exosomes 

  • High-efficiency removal of endotoxin and DNA 

  • Outstanding for preparative applications or analytical fingerprinting of process samples 

  • Sanitizable with 1 M NaOH

Request a Quote

CIMmultus® DEAE – Weak AEX

  • Weak anion exchanger 

  • Working range, pH 2–9 

  • Binds molecules with predominantly negative charge, repels molecules with predominantly positive charge 

  • Capture, intermediate, or polishing steps in either bind-elute or flow-through mode 

  • High capacity for plasmid DNA 

  • Sanitizable with 1 M NaOH

Request a Quote

CIMmultus® EDA – Weak AEX & Activated Group

  • Weak anion exchanger 

  • Working range, pH 2–9 

  • Binds molecules with predominantly negative charge, repels molecules with predominantly positive charge 

  • Capture, intermediate, or polishing steps in either bind-elute or flow-through mode 

  • High capacity for plasmid DNA 

  • Sanitizable with 1 M NaOH

Request a Quote

CIMmultus® EV – Strong AEX

  • Strong anion exchanger 

  • Working range, pH 2–13 

  • High capacity for exosomes 

  • High-efficiency reduction of host cell DNA 

  • High efficiency removal of viruses, endotoxins, and non-exosomal vesicles 

  • Sanitizable with 1 M NaOH

Request a Quote

Sartobind® IEX Membranes

High Speed IEX Capture/Polishing

Sartobind® IEX membranes enable rapid purification of various biomolecules.  Ready-to-use format minimizes set-up time and makes chromatography a simple and highly productive process step.


Sartobind® Q

Sartobind STIC® PA

Sartobind® S

Ligand

Quaternary ammonium

Primary amine

Sulfonic acid

IEX Type

Strong anion exchange

Salt-tolerant weak anion exchange

Strong cation exchange

Typical DBC at 10% breakthrough

BSA: 29 mg/mL

BSA: 50 mg/mL
DNA: 11 mg/mL

Lysozyme:25 mg/mL

pH stability

1-14

2-12

3-14

Request a Quote

Request a Quote

Request a Quote

Learn More

Learn More

Learn More

Sartobind® Q

Sartobind® Q is highly flexible and used  in a wide variety of processes such as the removal of HCP, DNA, viruses, and endotoxins. It is also applied for virus and nucleic acid (DNA/RNA) purification.
As with all Sartobind® membranes, the large pore structure is particularly suited to large biomolecules.

Request a Quote

Sartobind STIC® PA

Sartobind STIC® PA can operate at high conductivity and can remove DNA at up to 1.5 M NaCl.  DNA removal is possible even in the presence of phosphate buffers, which offers some unique processing opportunities.

Request a Quote

Sartobind® S

Sartobind® S membrane adsorber is approved for use in drug manufacturing to remove host cell proteins at high flow rates. As a cation exchanger, Sartobind® S displays aggregate removal capabilities and typically achieves at least ten-fold higher binding capacity for viruses and virus-like-particles (VLP).

Request a Quote

IEX Resins

A Range of Resins for Efficient and Cost-Effective Process Design

Ion exchange resins are used in the capture and purification chromatography steps of various biomolecules. Sartorius offers a comprehensive range of chemistries and bead matrices. The resins provide high capacity at short residence times in a flexible range of conductivities up to 12 – 15 mS/cm. Unique salt-tolerant matrices, like HyperCel Star AX anion exchange resin, allow simple process integration without the need for additional feedstream manipulation. 

Q Ceramic HyperD®

HyperCel STAR AX

DEAE Ceramic HyperD®

CM Ceramic HyperD®

Ligand

Quaternary amine

Primary amine

Diethylaminoethyl

Carboxymethyl

IEX type

Strong anion exchange

Weak salt-tolerant anion exchange

Weak anion exchange

Weak cation exchange

Working conductivity

4-8 mS/cm

Salt tolerant: 2-15 mS/cm

4-8 mS/cm

Salt tolerant: up to 10-19 mS/cm

Bead material


Ceramic polymer composite (50 µm)

Rigid cellulose
(80 µm)

Ceramic polymer composite (50 µm)

Ceramic polymer composite (50 µm)

DBC at 10% breakthrough

BSA ≥85 mg/mL

BSA ≥100 mg/mL

BSA ≥85 mg/mL


IgG ≥60 mg/mL

Request a Quote

Request a Quote

Request a Quote

Request a Quote

Learn More

Learn More

Learn More

Learn More

Q Ceramic HyperD®

Q Ceramic HyperD® is a strong anion exchanger that is suited for the removal of anionic impurities (i.e., host cell protein and DNA). Its 50 µm base bead offers a high resolution for purification applications when required.

Request a Quote

DEAE Ceramic HyperD®

DEAE Ceramic HyperD® is a weak anion exchanger for purification. Changes in conductivity and pH can optimize purification steps by utilizing the charge change on protein and ligand.

Request a Quote

CM Ceramic HyperD®

CM Ceramic HyperD® is a weak cation exchanger for polishing applications. Changes in conductivity and pH can optimize purification steps by utilizing the charge change on protein and ligand.  

Request a Quote

HyperCel STAR AX

HyperCel Star AX is a salt-tolerant anion exchanger with a primary amine as functional group. It can easily be integrated into a purification workflow without feedstream manipulation.

Request a Quote

Consult Our Experts

icon-chromatography-resin

Chromatography Resins

Learn More
icon-chromatography-membrane

Chromatography Membranes

Learn More
icon-chromatography-analytical

Biomolecule Analysis Tools

Learn More