Ion Exchange Chromatography
Ion exchange matrices are the first step in capture and polishing chromatography of various biomolecules. Satorius offers a comprehensive platform of resins, chromatography membranes, and monoliths to match nearly any application:
- Resins exhibit ideal binding capacities for small and medium-size proteins.
- Membranes and monoliths have a higher binding capacity for viruses, virus-like particles, and other large complex molecules while also delivering higher productivity thanks to reduced processing times.
- Unique salt-tolerant matrices like HyperCel Star AX anion exchange resin and Sartobind STIC® PA membrane provide simple process integration without additional feedstream manipulation.
Salt-tolerant matrices reduce or eliminate dilution or adjustment steps
Plug-and-play chromatography matrices allow high-speed processing without sacrificing capacity
Ion exchange matrices are widely established in various commercial processes
The Right Solution for Your Ion Exchange Chromatography Process
Ion exchange matrices play a key role in bioprocessing for capture and purification steps. While many ion exchange matrices are available, selecting the suitable matrix for your application ensures streamlined process development, scale-up, and commercialization.
Whether you are searching for high capacity or are working with large molecules, Sartorius has an ion exchange matrix to fit your purification needs.
Application | Capture and purification of large biomolecules | Capture of large biomolecules, flow-through purification of all biomolecules. Complementary solution to monoliths for large biomolecule purification | Capture and purification of small and medium biomolecules |
Implementation | Intrabatch multi-use, single-use between batches | Intrabatch multi-use, single-use between batches | Multi-use |
Ease of use | Ready-to-use | Ready-to-use | Bulk material (simplified packing) |
Scalability | Wide range of device sizes | Capsules and cassettes for linear scale-up | Wide range of column sizes |
Monoliths for IEX Chromatography
IEX Monolithic Chromatography
Benefit from all the attributes of monolithic chromatography with a range of strong and weak anion and cation exchangers to meet the needs for purification of large biomolecules.
Channel sizes | 1.3 | 2 | 6 µm | 1.3 | 2 | 6 µm | 1.3 | 2 | 6 µm | 2 µm | 2 µm |
Column volume | 1 mL – 8 L | 1 mL – 8 L | 1 mL –8 L | 1 mL – 8 L | 1 mL – 8 L |
Working range, pH | 2 – 13 | 2 – 13 | 2 – 9 | 2 – 9 | 2 – 13 |
Functionality | Strong cation exchange | Strong anion exchange | Weak anion exchange | Weak anion exchange | Strong anion exchange |
Typical application | Viruses, (i.e. AAV), | Viruses, (i.e. AAV), | pDNA | pDNA | Exosomes |
CIMmultus® SO3 – Strong CEX
Strong cation exchanger
Working range, pH 2–13
Binds molecules with predominantly positive charge, repels molecules with predominantly negative charge
Capture, intermediate, or polishing steps in either bind-elute or flow-through mode
High capacity for purification of virus and virus-like particles
Sanitizable with 1 M NaOH
CIMmultus® QA – Strong AEX
Strong anion exchanger
Working range, pH 2–13
Binds molecules with predominantly negative charge, repels molecules with predominantly positive charge
Capture, intermediate, or polishing steps in either bind-elute or flow-through mode
High capacity for virus particles, IgM, exosomes
High-efficiency removal of endotoxin and DNA
Outstanding for preparative applications or analytical fingerprinting of process samples
Sanitizable with 1 M NaOH
CIMmultus® DEAE – Weak AEX
Weak anion exchanger
Working range, pH 2–9
Binds molecules with predominantly negative charge, repels molecules with predominantly positive charge
Capture, intermediate, or polishing steps in either bind-elute or flow-through mode
High capacity for plasmid DNA
Sanitizable with 1 M NaOH
CIMmultus® EDA – Weak AEX & Activated Group
Weak anion exchanger
Working range, pH 2–9
Binds molecules with predominantly negative charge, repels molecules with predominantly positive charge
Capture, intermediate, or polishing steps in either bind-elute or flow-through mode
High capacity for plasmid DNA
Sanitizable with 1 M NaOH
CIMmultus® EV – Strong AEX
Strong anion exchanger
Working range, pH 2–13
High capacity for exosomes
High-efficiency reduction of host cell DNA
High efficiency removal of viruses, endotoxins, and non-exosomal vesicles
Sanitizable with 1 M NaOH
Sartobind® IEX Membranes
High Speed IEX Capture/Polishing
Sartobind® IEX membranes enable rapid purification of various biomolecules. Ready-to-use format minimizes set-up time and makes chromatography a simple and highly productive process step.
Ligand | Quaternary ammonium | Primary amine | Sulfonic acid |
IEX Type | Strong anion exchange | Salt-tolerant weak anion exchange | Strong cation exchange |
Typical DBC at 10% breakthrough | BSA: 29 mg/mL | BSA: 50 mg/mL | Lysozyme: 25 mg/mL |
Short term pH stability | 1-14 | 2-12 | 3-14 |
Sartobind® Q
Sartobind® Q is highly flexible and used in a wide variety of processes such as the removal of HCP, DNA, viruses, and endotoxins. It is also applied for virus and nucleic acid (DNA/RNA) purification.
As with all Sartobind® membranes, the large pore structure is particularly suited to large biomolecules.
Sartobind® Q Mini
The new Sartobind® Q Mini provides 10|20 mL membrane volume, which allows bioprocess customers easier scale-up and is a perfect fit for the production of diagnostic products. Sartobind® Mini is directly scalable with the existing Sartobind® portfolio which ranges from process development (Nano) to commercial manufacturing (Jumbo and cassettes).
Sartobind STIC® PA
Sartobind STIC® PA can operate at high conductivity and can remove DNA at up to 1.5 M NaCl. DNA removal is possible even in the presence of phosphate buffers, which offers some unique processing opportunities.
Sartobind STIC® PA Mini
The new Sartobind STIC® PA Mini provides 10 mL membrane volume, which allows bioprocess customers easier scale-up and is a perfect fit for the production of diagnostic products. Sartobind® Mini is directly scalable with the existing Sartobind® portfolio which ranges from process development (Nano) to commercial manufacturing (Jumbo and cassettes).
Sartobind® S
Sartobind® S membrane adsorber is approved for use in drug manufacturing to remove host cell proteins at high flow rates. As a cation exchanger, Sartobind® S displays aggregate removal capabilities and typically achieves at least ten-fold higher binding capacity for viruses and virus-like-particles (VLP).
Sartobind® S Mini
The new Sartobind® S Mini provides 10|20 mL membrane volume, which allows bioprocess customers easier scale-up and is a perfect fit for the production of diagnostic products. Sartobind® Mini is directly scalable with the existing Sartobind® portfolio which ranges from process development (Nano) to commercial manufacturing (Jumbo and cassettes).
IEX Resins
A Range of Resins for Efficient and Cost-Effective Process Design
Ion exchange resins are used in the capture and purification chromatography steps of various biomolecules. Sartorius offers a comprehensive range of chemistries and bead matrices. The resins provide high capacity at short residence times in a flexible range of conductivities up to 12 – 15 mS/cm. Unique salt-tolerant matrices, like HyperCel Star AX anion exchange resin, allow simple process integration without the need for additional feedstream manipulation.
Ligand | Quaternary amine | Primary amine | Diethylaminoethyl | Carboxymethyl |
IEX type | Strong anion exchange | Weak salt-tolerant anion exchange | Weak anion exchange | Weak cation exchange |
Working conductivity | 4-8 mS/cm | Salt tolerant: 2-15 mS/cm | 4-8 mS/cm | Salt tolerant: up to 10-19 mS/cm |
Bead material | Ceramic polymer composite (50 µm) | Rigid cellulose | Ceramic polymer composite (50 µm) | Ceramic polymer composite (50 µm) |
DBC at 10% breakthrough | BSA ≥85 mg/mL | BSA ≥100 mg/mL | BSA ≥85 mg/mL | IgG ≥60 mg/mL |
Q Ceramic HyperD®
Q Ceramic HyperD® is a strong anion exchanger that is suited for the removal of anionic impurities (i.e., host cell protein and DNA). Its 50 µm base bead offers a high resolution for purification applications when required.
HyperCel STAR AX
HyperCel Star AX is a salt-tolerant anion exchanger with a primary amine as functional group. It can easily be integrated into a purification workflow without feedstream manipulation.
DEAE Ceramic HyperD®
DEAE Ceramic HyperD® is a weak anion exchanger for purification. Changes in conductivity and pH can optimize purification steps by utilizing the charge change on protein and ligand.
CM Ceramic HyperD®
CM Ceramic HyperD® is a weak cation exchanger for polishing applications. Changes in conductivity and pH can optimize purification steps by utilizing the charge change on protein and ligand.
