Ion Exchange Chromatography
Discover Products Tailored to Your Needs
Ion exchange (IEX) matrices are the first step in capture and polishing chromatography of various biomolecules. Satorius offers a comprehensive platform of resins, chromatography membranes, and monoliths to match nearly any application:
- Resins exhibit ideal binding capacities for smaller molecules like small and medium-size proteins.
- Membranes and monoliths offer superior binding capacity for viruses, virus-like particles, and other large complex molecules, while enabling faster processing for increased productivity.
- Unique salt-tolerant matrices like HyperCel Star AX anion exchange resin and Sartobind STIC® PA membrane provide simple process integration without additional feedstream adjustment.
Ion Exchange Chromatography Portfolio
The Right Solution to Selectively Purify your Target Molecule
Discover how the diversity of matrices and ligand combinations of our ion exchange chromatography products can help simplify your downstream process.
High Speed IEX Capture and Polishing
Sartobind® IEX membranes enable rapid purification of various biomolecules. The ready-for-use format minimizes set-up time and makes chromatography a simple and highly productive process step.
| Sartobind® Q | Sartobind STIC® PA | Sartobind® S | Sartobind Convec® D | |
|---|---|---|---|---|
Ligand | Quaternary ammonium | Primary amine | Sulfonic acid | Diethylamine |
IEX Type | Strong anion exchange | Salt-tolerant weak anion exchange | Strong cation exchange | Weak anion exchange |
Typical DBC 10% | BSA: 29 mg/mL | BSA: 50 mg/mL DNA: 11 mg/mL | Lysozyme: 25 mg/mL | Lentivirus: 2 x 1012 VP/mL |
| Short-Term pH Stability | 1-14 | 2-12 | 3-14 | 3–14 |
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A Range of Resins for Efficient and Cost-Effective Process Design
Ion exchange resins are used in the capture and purification chromatography steps of various biomolecules. Sartorius offers a comprehensive range of chemistries and bead matrices. The resins provide high capacity at short residence time in a flexible capacity in a range of conductivities up to 12 – 15 mS/cm.
| Q Ceramic HyperD® | HyperCel STAR AX | DEAE Ceramic HyperD® | CM Ceramic HyperD® | |
|---|---|---|---|---|
| Ligand | Quaternary amine | Primary amine | Diethylaminoethyl | Carboxymethyl |
| IEX type | Strong anion exchange | Weak salt-tolerant anion exchange | Weak anion exchange | Weak cation exchange |
| Working conductivity | 4-8 mS/cm | Salt tolerant: 2-15 mS/cm | 4-8 mS/cm | Salt tolerant: up to 10-19 mS/cm |
Bead material
| Ceramic polymer composite (50 µm) | Rigid cellulose (80 µm) | Ceramic polymer composite (50 µm) | Ceramic polymer composite (50 µm) |
| DBC at 10% breakthrough | BSA ≥85 mg/mL | BSA ≥100 mg/mL | BSA ≥85 mg/mL
| IgG ≥110 mg/mL |
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IEX Monolithic Chromatography
Benefit from all the attributes of monolithic chromatography with a range of strong and weak anion and cation exchangers to meet the needs for purification of large biomolecules.
| CIMmultus® SO3 | CIMmultus® QA | CIMmultus® DEAE | CIMmultus® EDA | |
|---|---|---|---|---|
| Channel sizes | 1.3 | 2 | 6 µm | 1.3 | 2 | 6 µm | 1.3 | 2 | 6 µm | 2 µm |
| Column volume | 1 mL – 8 L | 1 mL – 8 L | 1 mL –8 L | 1 mL – 8 L |
| Working range, pH | 2 – 13 | 2 – 13 | 2 – 9 | 2 – 9 |
Functionality
| Strong cation exchange | Strong anion exchange | Weak anion exchange | Weak anion exchange |
Typical application
| Viruses, (i.e. AAV), and VLPs | Viruses, (i.e. AAV), and VLPs | pDNA | pDNA |
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CIMmultus® SO3 – Strong CEX
Strong cation exchanger
Working range, pH 2–13
Binds molecules with predominantly positive charge, repels molecules with predominantly negative charge
Capture, intermediate, or polishing steps in either bind-elute or flow-through mode
High capacity for purification of virus and virus-like particles
Sanitizable with 1 M NaOH
CIMmultus® DEAE – Weak AEX
Weak anion exchanger
Working range, pH 2–9
Binds molecules with predominantly negative charge, repels molecules with predominantly positive charge
Capture, intermediate, or polishing steps in either bind-elute or flow-through mode
High capacity for plasmid DNA
Sanitizable with 1 M NaOH
CIMmultus® EDA – Weak AEX & Activated Group
Weak anion exchanger
Working range, pH 2–9
Binds molecules with predominantly negative charge, repels molecules with predominantly positive charge
Capture, intermediate, or polishing steps in either bind-elute or flow-through mode
High capacity for plasmid DNA
Sanitizable with 1 M NaOH
CIMmultus® QA – Strong AEX
Strong anion exchanger
Working range, pH 2–13
Binds molecules with predominantly negative charge, repels molecules with predominantly positive charge
Capture, intermediate, or polishing steps in either bind-elute or flow-through mode
High capacity for virus particles, IgM, exosomes
High-efficiency removal of endotoxin and DNA
Outstanding for preparative applications or analytical fingerprinting of process samples
Sanitizable with 1 M NaOH
Supporting Products & Services
Learn More About Ion Exchange Chromatography
Highlight Assets
Related Assets
Host Cell Protein Removal - A Comparison Between Sartobind STIC® PA and Sartobind® Q
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Endotoxin Removal - Ion Exchange Chromatography With Sartobind® Membrane Adsorbers
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Capture of an Acid-Sensitive Protein From CHO Cell Culture Supernatant
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High Throughput Chromatography Resin Screening and Purification Process Optimization
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Optimizing Adenovirus Purification Processes - Using Sartobind® Q and STIC PA Anion Exchange Membranes
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Frequently Asked Questions
Ion exchange chromatography separates biomolecules like proteins based on their net charge. In anion exchange chromatography, the stationary phase is charged positively. Therefore, negatively charged biomolecules bind to the stationary phase. In cation exchange chromatography, the stationary phase is charged negatively. Thus, positively charged biomolecules bind to the stationary phase.
Ion exchange chromatography is a universal tool that can be applied in many purification processes. For example, in mAb processes, it is used to polish chromatography following the capture step. The most common examples are virus removal, DNA removal, host cell protein removal, aggregate removal, and endotoxin removal. For other modalities, ion exchange chromatography is also commonly used as capture step, for example, viruses, VLPs, and pDNA.
Usually, with increased salt content, the charge of the stationary phase and, therefore, the ability to bind, decreases. Typically, high salt conditions are also used for the elution. Ion exchangers that are considered salt-tolerant can bind, at higher salt concentrations than traditional ion exchangers. Salt-tolerant stationary phases are easier to implement and reduce or eliminate the need for dilution for adjustment steps.
If the pH of the medium is above the isoelectric point of the biomolecule, the biomolecule has a negative net charge. If the pH of the medium is below the isoelectric point of the biomolecule, it has a positive net charge. The pH of the medium is decisive for the binding conditions of the biomolecule to the stationary phase.
Sartorius offers ion exchange ligands on membranes, resins, and monoliths. The choice of matrix is dependent on the use case. Resins offer the best performance for smaller molecules, while membranes and monoliths offer the best performance for larger molecules or whenever high flow rates are required. Our experts are happy to assist in selecting the right product.
