Protect Your Cell Culture Process With Virosart® Media

Virus Retention for Cell Culture Media

Virus-retentive filtration is a highly effective method for reducing contamination in cell culture media. Designed specifically for chemically defined formulations, Virosart® Media employs an optimized polyethersulfone membrane to deliver high-throughput filtration, with average capacities of 800–1,000 L/m² achieved in just four hours.

Virosart® Media ensures reliable viral risk mitigation by efficiently removing both small, non-enveloped viruses such as MVM (LRV ≥ 4 log₁₀) and larger enveloped viruses such as MuLV (LRV ≥ 6 log₁₀). Available as sterile mid-scale and process-scale modules, as well as pre-assembled virus filter transfer units, Virosart® Media simplifies operation and maximizes efficiency.

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Challenges in Virus Risk Mitigation for Media Preparation

Multiple bioreactor contaminations reported over the past several years have been caused by small, non-enveloped viruses like MVM and vesivirus, originating from raw materials.

A New Virus Risk Mitigation Strategy

A new approach involves removing viruses from the media immediately after preparation, before it enters the bioreactor, to prevent potential contamination. Virus filtration is a proven and well-established technology in the market, capable of removing all types of viruses larger than 20 nm.

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Product Overview

Virosart® Media is a virus-retentive filter specifically designed for upstream applications. Unlike virus filters originally developed for downstream processes, this newly developed media filter addresses current upstream bottlenecks and offers a cost-effective solution with the highest filter capacity for cell culture media.

Virus Risk Mitigation in Media Preparation for:

  • Chemically defined cell culture media
  • Addition of media supplements, such as CaCl₂
  • Both powder and liquid media

Optimal protection for the bioreactor:

  • ≥ 4 log₁₀ reduction for small non-enveloped viruses
  • ≥ 6 log₁₀ reduction for large enveloped viruses

New high-performance PES membranes:

  • Economic solution for upstream virus media filtration
  • High capacities and flow rates
  • No impact on cell culture performance

Sterile delivery due to gamma-irradiatable capsule design:

  • Ready to use
  • Easy implementation into single-use processes

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Performance Deep Dive

Technical Validation

Performance

In total >15 different commercially available cell culture media were tested with the Virosart® Media. 

Retentive Capacities

Virosart® Media shows high retention for all tested viruses. The reliable performance of the media virus retentive filter helps to mitigate risk and enhance speed from process to commercial scale.

OrganismDescriptionLRV
MVMRelevant and model virus for small non-enveloped virus  ≥ 4 log₁₀
MuLVModel virus for large enveloped viruses     ≥ 6 log₁₀

Knowledge Base

Highlight Asset

Poster

Mitigate Virus Contamination Risks for Advanced Therapies

Ensuring virus-free raw materials for ATMP production by integrating nanofiltration as an upstream risk mitigation tool.

Download Library

Article

Virus Risk Mitigation in Cell Culture Media

PDF | 527.9 KB
Application Note

Filterability Performance

PDF | 1.3 MB
Application Note

Retention Characteristics

PDF | 799.0 KB
Application Note

Influence on Media Components on Filtration

PDF | 741.7 KB
Application Note

Impact on Cell Growth

PDF | 1.1 MB
Application Note

Lot to Lot Performance Consistency

PDF | 1.3 MB
Application Note

Virus Clearance Under Challenging Conditions

PDF | 860.8 KB
Application Note

Filtration of Calcium Chloride

PDF | 761.7 KB
Datasheet

Virosart® Media Datasheet

PDF | 919.7 KB
Poster

New Solutions for Virus Risk Mitigation in Cell Culture Media

PDF | 717.6 KB
Poster

Case Study: Virus Risk Mitigation in Cell Culture Media

PDF | 919.1 KB
Poster

Contamination Risk Mitigation in Cell Culture Media Preparation

PDF | 1.1 MB
Flyer

Virosart® Virus Filters - Reliable Retention Under All Circumstances

PDF | 528.4 KB

Training Videos

Tutorial

Virosart® HF Lab Module Product Tutorial

Discover the steps to effectively install and execute virus filtration trials. Gain valuable insights to enhance your filtration process.

Tutorial

Virosart® HF Process Module Product Tutorial

Learn the installation and execution of virus filtration at commercial scale with the expert handling tips in our comprehensive guide.

Frequently Asked Questions

Virosart® Media filters are primarily used for virus-retentive filtration of chemically defined cell culture media. They are implemented after media preparation and before transfer to the bioreactor, and are suitable for both in-line filtration (e.g., perfusion) and batch filtration processes.

Virosart® Media is highly effective in virus removal, providing logarithmic reduction values of ≥ 4 log₁₀ for small non-enveloped viruses such as MVM and ≥ 6 log₁₀ for large enveloped viruses like MuLV. This ensures reliable viral risk mitigation in upstream media virus filtration processes.

Yes, Virosart® Media filters are designed for easy integration into single-use processes. They are available in gamma-irradiatable capsule designs, enabling sterile delivery and quick installation, making them ideal for single-use applications.

The performance of Virosart® Media filters can be influenced by the specific media used, including additives like poloxamere. An efficient pre-filtration step, such as using Sartopore® 2 XLM, can enhance the capacity of the final virus filter. The optimal pre-filter to final filter ratio should be determined during the development of the process step.

Virosart® Media filters are available in lab, mid-scale, and process modules. The lab module (5.0 cm²) is used for scale-up studies, growth studies, and small-scale production. The mid-scale module (0.3 m²) is suitable for optimization and GLP spiking studies, while the process module (1.0 m²) is designed for large-scale manufacturing. Each module is available in sterile and non-sterile versions, with various connector options to suit different applications.

Supporting Products & Services

Large Scale Single-Use Virus Filtration

Maxicaps® MR Virosart® for large scale filtration eliminates 90% formerly needed tubings | connections and minimizes risks and installation efforts.

Extractables and Leachables Testing

Ensure the safe adoption of single-use systems in biopharmaceutical processes.

Find the Right Virus Filter for Your Needs

Reliable retention under all circumstances with our Virosart® virus filters.

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