Speed Up Your AAV Vector Development and Manufacturing With a HEK293 Suspension-Based Platform

Adeno-associated viral vectors (AAVs) are among the most widely used vectors for gene therapy applications. While cultivation systems such as roller bottles, CellSTACKs (Corning), CellFactories (Nunc, ThermoFisher
Scientific), fixed bed reactors, or microcarrier-based cell culture are extensively used in large-scale adherent production of viral vectors such as AAV, there are many reasons to adapt your process to suspension:

• Eliminates the need for serum and its associated negative aspects(animal-origin, transmissible spongiform encephalopathy/bovine spongiform encephalopathy risk, costs, ethics, lot-to-lot variation,
  sourcing, etc.)
• Allows flexibility regarding scale and volumes per process Improved scalability offers lower operation costs compared to multiple parallel processes
• Using controlled cultivation systems improves optimization potential Enhanced process control enables easy documentation of parameters for regulatory purposes