Microsart® Mycoplasma Detection Designed for fast, reliable detection using real-time PCR
Viable but non-culturable (VBNC) Mycoplasma can go undetected when using the traditional method of mycoplasma culturing, risking false-negative results. Our Microsart® qPCR kits offer a method for early detection of mycoplasma contamination. These kits are fast, highly specific, sensitive, and compliant with international guidelines.
Microsart® Mycoplasma qPCR detection kits: a rapid and guideline-compliant solution for Mycoplasma detection.
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Mycoplasma Testing Product Highlights
Microsart® ATMP Mycoplasma
Rapid Mycoplasma Detection in 3 Hours
Microsart® ATMP Mycoplasma is designed to rapidly detect total mycoplasma in cell cultures, cell culture-derived biologicals, and ATMPs. The technology is based on real-time polymerase chain reaction (PCR).
Why Choose the Microsart® ATMP Mycoplasma qPCR Detection?
- Microsart® ATMP Mycoplasma enables a reliable and sensitive detection of Mycoplasma DNA
- Reliable Results in 3 Hours — Avoid lengthy 28-day cultivation protocols with Microsart® Mycoplasma qPCR Kits. TaqMan® probes ensure the highest qPCR specificity without the need for a melting-curve analysis
- The Only Non-infectious, Quantified Validation Standards — Validation standards for required mycoplasma species in Microsart® Validation Standard kits are lyophilized and non-infectious. We provide step-by-step, matrix-specific validation guidance from Sartorius qPCR experts.
- Compliance with International Standards — Microsart® Mycoplasma Kits are validated according to EP 2.6.7 for sensitivity, specificity, and robustness. They also fulfill the criteria of international authorities, such as USP and JP.
- Reduce Pipetting Steps — The ready-to-use master mix has the DNA for internal control already included. Avoid false-negative results and minimize the number of reactions you need to run.
- Use Your Existing Equipment — Microsart® Mycoplasma qPCR kits can be used with any qPCR cycler able to detect the fluorescent dyes FAM® and ROX®. Our clear, color-coded reagents guide the user through the short and simple protocol. Lyophilized reagents ensure consistent quality without the need for freezer storage.
Micorsart® AMP Extraction
The kit was especially designed to complement the Microsart® AMP Mycoplasma, Microsart® ATMP Mycoplasma and Microsart® RESEARCH Mycoplasma qPCR kits and to provide best performance in means of sensitivity and robustness for the detection of mycoplasma within a test sample.
Cyclus® Bead Extraction
Magnetic Bead Based Mycoplasma Nucleic Acid extraction
Cyclus® Bead Extraction is a magnetic bead-based nucleic acid extraction kit developed for the efficient and automated isolation of Mycoplasma nucleic acids from various sample matrices, such as cell cultures, cell culture supernatants, and biopharmaceuticals.
Why Choose Cyclus® Bead Extraction ?
- Flexible Extraction Methods — Extraction can be performed manually using a magnetic rack or automatically using the KingFisher™ Flex.
- Designed for Sartorius qPCR Kits — Cyclus® Bead Extraction is designed for use with Sartorius qPCR kits, including Microsart® AMP Mycoplasma, Microsart® ATMP Mycoplasma, and Microsart® RESEARCH Mycoplasma.
- Fast Results — Extraction time is approximately 1.5 hours.
- Regulatory-Compliant Preparation — Sample preparation for Mycoplasma release testing is in accordance with EP 2.6.7.
- Convenient & Stable Kit — The kit includes 100 extractions, has a shelf life of 2 years, and is stable at room temperature.
Microsart® Mycoplasma Versions
Choose the Right Microsart® for Your Needs
Ensure Cell Culture Integrity
The Dangers of Mycoplasma Contamination
Mycoplasma contamination can quietly compromise experiments, change cell behavior, and invalidate results without any obvious signs. More than 95% of contaminations arise from just six species and can spread through water baths, pipettes, or even lab personnel. Traditional testing methods can take weeks. Microsart® Mycoplasma offers reliable detection in just 3 hours, allowing you to respond quickly and safeguard your work.
Resources for Clean and Reliable Cell Cultures
Featured Resources
Mycoplasma Testing Product Documentation & Literature
Rapid MycoplasmaDetection: Microsart® AMP Extraction and Microsart® ATMP Mycoplasma
PDF | 815.3 KB
Rapid detection of Mycoplasma and other cell wall-less bacterial species via quantitative PCR
PDF | 894.8 KB
Rapid MycoplasmaDetection: Microsart® AMP Extraction and Microsart® ATMP Mycoplasma
PDF | 815.3 KB
