Smarter Technologies for Advancing the Next Generation of CAR-T Therapies
For immunotherapy researchers, Sartorius in vitro assay solutions reduce complexity through deeper insights, streamlined workflows via the collapse of various assays, validation of results by complementary technologies, and robust multiparametric software analysis.
The quest to extend the success of CD19 directed CAR-T therapy to other hematologic malignancies (e.g. CD123-specific ALL/AML), and ultimately solid tumors, ideally with off-the-shelf CAR-T solutions, is of paramount importance.
In order to achieve this, appropriate R&D in vitro methods are required to rapidly identify novel tumor antigens and engineer effective, tumor-seeking T-cells. This process includes evaluating various CAR delivery methods used to introduce genetic modifications as well as the continuous assessment of specificity, toxicity and activity of these modulated T-cells.
Also, for increased safety, we need to ensure through constant monitoring that, at no point in the experimental process, we allow for contamination (e.g. mycoplasma).
The iQue® advanced flow cytometry and Incucyte® automated, continuous live-cell analysis platforms (instrumentation, reagents and software solutions), in combination with rapid (< 3 hours) contamination testing and plate seeding (Picus® & Picus® Nxt series) solutions form the optimal combination for your CAR-T research needs.
CAR-T Application Highlights
Disrupting the Solid Tumor Environment Using CAR-T Therapies
CAR-T experts about the challenges and novel strategies for targeting CAR T and related ACT platforms to the solid tumor environment.
From Boutique to Global: Chimeric Antigen Receptor T-Cells as a Model for Clinical Development and Commercialization
Listen to Univ. of Pennsylvania's Dr. Bruce Levine as he discusses the science of CAR T-cells and their huge global impact.
Gaining Critical Characterization Insights for Development of CAR-T Therapies for Solid Tumors
Get expert views from leading CAR-T scientists as they share their valuable insights on the current status.
Challenges and Solutions in CAR-T Discovery and Development
Learn about the challenges of developing CAR-T therapies and innovative solutions to address these challenges.
Discover Our Solutions
Faster, Smarter Screening for CAR-T: iQue® Advanced Flow Cytometry Platform
A high throughput CAR-T cell analysis platform suited for T cell activation & cytokine profiling assays and multiplexed phenotypic analysis.
Speed — Fasted plate sampling, integrated analysis, multiplexed no-wash assays, and novel data reduction tools
Miniaturization — Conserve precious samples and reagents by sampling as little as a single microliter from miniaturized assay volumes
Content — High content, multiplexed analysis of cells, beads and secreted proteins
Usability — Simple, scalable, multi-user environment with walkaway automation, comprehensive analysis and visualization tools
Insight — ForeCyt Software with dynamic visualization tools provides rapid identification of wells of interest, even if run on multiple plates acquired on different days
High throughput, multiplex screening and testing of cell-mediated cytotoxicity, targeting, and CAR transduction to get from sample to actionable results - in record time.
Validate your CAR-T Hits: Incucyte® Live-Cell Analysis System
Real-time, automated imaging and analysis of CAR-T cell homing (chemotaxis), activation and function, directly from inside your incubator.
- Real-time continuous analysis means you never miss a data point
- Profile cell-specific and time-dependent biological activity
- Visualize and validate results with images and movies
- Multiplex measurements in 96- and 384-well assay formats
Validate your HT screen results by visualizing in 2D/3D CAR-T cytotoxicity , apoptosis, and T-cell health & proliferation with integrated hardware & software solutions.
Engineered for the Culture and Expansion of Lymphocytes: C4Cell®️ Nutri-T Medium
The 4Cell®️ Nutri-T medium is a xeno-free, serum-free medium, optimized for the activation and expansion of PBMC's (T cells, CAR-T) and TILs in ex vivo cell culture conditions.
4Cell®️ Nutri-T eliminates the need for serum addition, and thus simplifies the process, lowers the regulatory risk, and reduces the associated logistical burden. 4Cell®️ Nutri-T was developed using actual cancer patient cells, and it shows excellent performance for PBMC, TIL and CAR-T, even at low initial seeding densities.
Test & Monitor for Contamination: Microsart® Detection Kits
Providing fast contaminant detection during CAR-T discovery & development for increased safety. Rapid, highly specific & sensitive.
- Detect >95% of all known bacteria in one test
- Results in 3 hours: prior to treatment
- Specific TaqMan® probes reduce false-positives
- Non-infectious validation standards
- Less pipetting: controls already included
Sensitive applications involving CAR-T cells, cancer cell lines, spheroids, require optimal external environments, that are free of contaminants. Make sure that your cells are at their physiological optimum.
Plate Seeding: Picus® and Picus® Nxt Electronic Pipettes
Your pipette can be an extension of your hand, and is one of the most used (and most personal) tools in your laboratory. Manual and electronic pipette options enable multiple plate setups to optimize CAR-T cell culture and analysis conditions, while maximizing comfort and minimizing strain injuries.
Precise E:T ratios for correct % specific lysis calculations are crucial, hence minimizing the risk of error to ensure accuracy in seeding across a large number of plates is an absolute prerequisite for excellent results.
Other Featured CAR-T Research Applications
CAR-T Discovery & Development Resources
View webinars, eBooks, infographics, literature and other educational material in our CAR-T Discovery & Development Resources area.