Monolithic Chromatography
Process Improvement With Monolithic Chromatography
CIM® (Convective Interaction Media) monoliths are designed as unique, single-unit structures – making them ideal for purifying very large biomolecules with a very small lab footprint. Large molecules are used commonly in modern gene therapy and vaccines products, and include virus particles, vesicles, RNA, plasmids, and other forms of DNA. These molecules require dedicated solutions for chromatographic purification.
The Sartorius family of CIM® monoliths is ideal for preparatory and analytical applications as well as immobilization screening. Pre-configured product packs are also available, a combination of columns and a protocol to suit specific needs.
Monolithic Chromatography in 3D
Large biomolecules are becoming increasingly popular in the biotherapeutic industry. Innovative solutions - such as monolithic chromatography - are needed to purify these new modalities.
Join us on a journey through the monolith matrix to discover how convective chromatography supports the robust separation of large biomolecules.
High resolution capabilities in combination with low shear flow properties for quality yields
Increased throughput from improved yields and faster run times, benefitting from high capacity and ready-to-use formats
Ready-to-use cGMP formats with proven scalability from 1 mL to 40 L
A robust tool for the purification of biomolecules in the nanometer range
Monoliths by Chemistry and Application
A Selection of Monoliths By Chemistry
CIMmultus® QA | CIMmultus® OH | CIMmultus® Oligo-dT | CIMmultus® PrimaS |
CIMmultus® DEAE | CIMmultus® C4-HLD | CIMmultus® H-Bond | |
CIMmultus® EV | |||
CIMmultus® EDA | |||
CIMmultus® SO3 | |||
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Monolithic Chromatography Across Multiple Applications
AAV & Other Viral Vectors | mRNA | pDNA | Exosomes |
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CIMmultus® OH | CIMmultus® EV | ||
CIMmultus® PrimaS | Cornerstone® Exosome | ||
HiP2 Plasmid Process Pack |
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CIM® monoliths contain highly cross-linked, porous poly-methacrylate with well-defined channel size distribution. High surface accessibility of binding sites allows capacities beyond resin-based columns for large biomolecules, as well as rapid convection-based mass transfer.
With flow-independent performance, low back-pressure, and versatility our monolithic columns facilitate faster purification of your viruses or nucleic acids. CIM® monoliths are available in a variety of chemistries and sizes ranging from screening to commercial processing for higher yields and improved speed.
Monoliths for IEX Chromatography
IEX Monolithic Chromatography
Benefit from all the attributes of monolithic chromatography with a range of strong and weak anion and cation exchangers to meet the needs for purification of large biomolecules.
Channel sizes | 1.3 | 2 | 6 µm | 1.3 | 2 | 6 µm | 1.3 | 2 | 6 µm | 2 µm | 2 µm |
Column volume | 1 mL – 8 L | 1 mL – 8 L | 1 mL –8 L | 1 mL – 8 L | 1 mL – 8 L |
Working range, pH | 2 – 13 | 2 – 13 | 2 – 9 | 2 – 9 | 2 – 13 |
Functionality | Strong cation exchange | Strong anion exchange | Weak anion exchange | Weak anion exchange | Strong anion exchange |
Typical application | Viruses, (i.e. AAV), | Viruses, (i.e. AAV), | pDNA | pDNA | Exosomes |
CIMmultus® SO3
CIMmultus® SO3 – Strong CEX
Strong cation exchanger
Working range, pH 2–13
Binds molecules with predominantly positive charge, repels molecules with predominantly negative charge
Capture, intermediate, or polishing steps in either bind-elute or flow-through mode
High capacity for purification of virus and virus-like particles
Sanitizable with 1 M NaOH
CIMmultus® QA
CIMmultus® QA – Strong AEX
Strong anion exchanger
Working range, pH 2–13
Binds molecules with predominantly negative charge, repels molecules with predominantly positive charge
Capture, intermediate, or polishing steps in either bind-elute or flow-through mode
High capacity for virus particles, IgM, exosomes
High-efficiency removal of endotoxin and DNA
Outstanding for preparative applications or analytical fingerprinting of process samples
Sanitizable with 1 M NaOH
CIMmultus® DEAE
CIMmultus® DEAE – Weak AEX
Weak anion exchanger
Working range, pH 2–9
Binds molecules with predominantly negative charge, repels molecules with predominantly positive charge
Capture, intermediate, or polishing steps in either bind-elute or flow-through mode
High capacity for plasmid DNA
Sanitizable with 1 M NaOH
CIMmultus® EDA
CIMmultus® EDA – Weak AEX & Activated Group
Weak anion exchanger
Working range, pH 2–9
Binds molecules with predominantly negative charge, repels molecules with predominantly positive charge
Capture, intermediate, or polishing steps in either bind-elute or flow-through mode
High capacity for plasmid DNA
Sanitizable with 1 M NaOH
CIMmultus® EV
CIMmultus® EV – Strong AEX
Strong anion exchanger
Working range, pH 2–13
High capacity for exosomes
High-efficiency reduction of host cell DNA
High efficiency removal of viruses, endotoxins, and non-exosomal vesicles
Sanitizable with 1 M NaOH
Monoliths for HIC
The Choice for Hydrophobic Interaction Chromatography (HIC)
Hydrophobic interaction chromatography (HIC) is a must for nucleic acid separations. When combined with the advantages of monolithic chromatography, HIC meets this need while also providing an excellent solution for the purification of large biomolecules including adenoassociated viruses (AAV).
Channel sizes | 1.3, 2, 6 µm | 2, 6 µm |
Column volume | 1 mL – 8 L | 1 mL – 8 L |
Working range, pH | 2 – 10 | 2 – 10 |
Functionality | Weak hydrophobic | Strong hydrophobic |
Typical application | Viruses, (i.e. AAV), and VLPs | Nucleic acids (pDNA, mRNA) |
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CIMmultus® OH – Hydrophobic Interaction Chromatography
Uncharged (neutral) ligand
Working range, pH 2–10
Retains very large solutes (virus particles, VLP, vesicles) in the presence of precipitating salts or polyethylene glycol
Elutes large solutes with high resolution in order of increasing size
Elution with descending salt or polyethylene glycol
Sanitizable with 1 M NaOH
CIMmultus® C4-HLD - Hydrophobic Interaction Chromatography
Strongly hydrophobic, (uncharged) ligand
Working range, pH 2–10
Highly effective for removing proteins from nucleic acids (pDNA, RNA)
Monoliths for Affinity Purification
Highly Specific Monolithic Affinity Purification
Monolithic columns combined with unique affinity ligands offer an unmatched solution for affinity purification of large biomolecules.
- Channel sizes: 2 µm
- Column volume: 1 mL – 8 L
- Working range, pH: 2 - 13
- Functionality: Thymine oligomer based affinity
- Application: mRNA
CIMmultus® Oligo dT – Affinity for RNA
Oligo dT ligands covalently bound to solid support
Hybridizes to poly-adenylated tail found on most eukaryotic mRNAs, or synthetized onto the molecule during IVT
Fast and efficient capture and purification of mRNA with a poly-adenylated tail, from various sources
Selectively removes impurities from samples
Convenient approach for initial purification of mRNA
Available with:
Mixed Mode Monolithic Columns
Unique Selectivity with Mixed Mode Monolithic Columns
The unique selectivity of mixed-mode monolithic columns ensures the proper purification of the most challenging large biomolecules.
Channel sizes | 2 µm | 1.3, 2 µm |
Column volume | 1 mL – 8 L | 1 mL – 8 L |
Working range, pH | 2 – 13 | 2 – 13 |
Functionality | Hydrogen bonding and anion exchange | Multimodal hydrogen donor-acceptor |
Application | mRNA | Viruses and extra-cellular vesicles |
CIMmultus® PrimaS
Multimodal chromatography ligand that combines elements of hydrogen bonding with anion exchange chromatography
Unique selectivities in many important product areas
Supports purification of single-stranded RNA at ambient temperature
Fractionates ssRNA by size
CIMmultus® H-Bond ADC
Multimodal hydrogen donor-acceptor
Working range, pH 2–13
Enables size-based separation of product and contaminants at acidic pH
Elution with increasing salt, pH, or sorbitol
Highly tolerant of salt
Outstanding DNA removal
Unique selectivity for preparative and analytical applications
Sanitizable with 1 M NaOH
Monolith Process Packs
Process Packs Kick-Start Your Purification Process
Process Packs support the development and scale-up of large molecule purification. They come with recommended CIMmultus® columns and detailed process instructions to achieve high-quality purification in a short time.
Column volume | 1 – 80 mL | 1 mL (larger on request) |
Included chemistries | CIMmultus® DEAE CIMmultus® C4-HLD | CIMmultus® EV |
Additional components | Detailed process instructions | Kryptonase DNAse Detailed process instructions |
HiP² Plasmid Process Pack™
HiP² Plasmid Process Pack™ is a ready-to-use, adjustable pDNA purification pack, to help develop your own pDNA purification process and transfer it to production.
Detailed experiments are included, saving save time and money.
The process is extremely flexible, and allows choosing only parts of the protocol that fit your needs. CIMmultus® HiP² Plasmid Process Pack™ contains one weak anion-exchange (DEAE) and one hydrophobic (C4 HLD) column and a purification protocol of your plasmid.
Cornerstone® Exosome Process Development Pack
Cornerstone® Exosome Process Development Pack is a ready-to-use, adjustable exosome purification pack to help you develop your own purification process and transfer to production.
Detailed experiments are included, saving time and reducing cost. Cornerstone® Exosome Process Development Pack contains enzyme-based reagent (Kryptonase), one ion-exchange (EV) 1 mL column, and a purification protocol of your sample.
For bigger volumes, please contact our sales team.
Related Assets
Application Notes
Miniaturisation of Trypsin Immobilised Enzymatic Reactor for Protein Digestion
PDF | 314.1 KB
Increasing Productivity of pDNA DSP Using Sample Displacement Chromatography
PDF | 459.9 KB
Recovery of pDIKE2 Plasmid for Hepatitis C Vaccine Using CIMmultus® C4 HLD Monolith
PDF | 338.5 KB
Poster
Implementing Chromatographic Methods for Evaluating Large-Scale Monolithic Columns for AAV Capsid Separation
PDF | 531.9 KB
Comparability Study of an Ion Exchange Monolith and Affinity Resin for the Purification of AAV8
PDF | 2.7 MB
