Integrated Production Process of mRNA from E. coli to Highly Purified mRNA
In vitro transcription (IVT) is the enzymatic process used for mRNA vaccine production. It differs from biological fermentation processes as it requires linearized plasmid DNA. This linear isoform is produced with restriction enzymes from open circular and supercoiled pDNA. However, employing a traditional pDNA manufacturing process, which removes linear and open-circular isoforms, reduces the production yield.
In this video Aleš Štrancar, Managing Director – BIA Separations, a Sartorius company, discusses treating pDNA and mRNA as a single production process. Learn about this new purification approach from E. Coli through mRNA production that leads to improved recoveries, extra-low protein impurities, and efficient dsRNA removal.
Key Learning Objectives
- Explore a new purification approach starting from E.coli through to mRNA production, resulting in improved recoveries, extra-low protein impurities, and very efficient dsRNA removal
- See the potential impact of implementing rapid analytics of the IVT reaction, including optimized conditions and improved transcription numbers
- Learn how optimized conditions for pDNA linearization minimize protein contamination, facilitate purification, and may prevent aggregate formation
Meet Our Expert
Managing Director | BIA Separations
Ales Strancar is the Managing Director of BIA Separations, a Sartorius company, and one of CIM's primary inventors (Convective Interaction Media), a new generation of chromatographic support. He has authored/co-authored more than 60 scientific papers dealing with separation and purification technologies. He is a co-author of 5 granted USA patents and their foreign equivalents in biomolecule separations and purification. As well he is a co-author of several book chapters dealing with novel chromatography technologies for biomolecule separation.