Extraction of mRNA From IVT Mixtures With CIMmultus^® Oligo dT Column

The increasing demand for messenger RNA (mRNA) as a therapeutic product requires larger production scales and more efficient extraction techniques. The fast and efficient way to purify polyadenylated mRNA using affinity chromatography on the CIMmultus^® Oligo dT column is presented. 

The poly-adenylated tail of mRNA interacts with covalently bound oligo dT ligands in high-salt loading conditions, where electrostatic repulsion between negatively charged backbones of both mRNA and oligo dT are reduced, and H-bonding in the T-A base pair is emphasized. High salt concentration also screens out attractive electrostatic interactions between mRNA and other components in the process sample, thus facilitating an aggregate reduction in purified products.