Viral Vaccine Cell Culture Media
Specialty Media Designed to Allow Maximum Cell Growth and Virus production
Viral vaccines are produced by viral infection of susceptible animal cells or by recombinant protein expression in suitable host cells. A variety of cell lines are used for production of viral vaccines, mostly cells derived from kidneys, such as Vero, MDCK, and HEK293. Others cell types can also be used (sf9, sf21, EB66®, ...) to produce recombinant protein vaccines, also called recombinant Subunit.
Specialty media offered in this category have been designed to allow maximum cell growth and virus production.
Cell lines from different sources may have specific nutritional requirements and may therefore prefer one medium over another. More than one medium formulation should be evaluated to determine the best option.
As a partner of the vaccine industry, we at Sartorius are committed to providing extensive media solutions for developing vaccines using these platforms.
HEK293 Media
Our high performing HEK media are designed to best fit with the high variability of HEK293 cell lines, the versatility of AAV and complexity of viral vector processes from early clinical phases to large commercial scale.
Vero Cell Proliferation
4Cell® NutriVero™ Flex 10 is a chemically defined, serum-free, animal component-free medium, developed to support Vero cell growth in 2D monolayers as well as in 3D microcarriers suspension culture systems and optimized for the production of viruses.
Superior MDCK Cell Proliferation
4Cell® MDCK CD Medium is a chemically defined, serum-free, protein-free, animal component-free, hydrolysatefree medium designed to the growth and infection of Madin-Darby Canine Kidney (MDCK) cells in suspension conditions, either for research use of for further manufacturing purposes.
4Cell® Insect Cell Proliferation
The 4Cell® Insect CD Medium is a complete, chemically-defined, serum-free, protein-free, animal component-free, hydrolysate-free medium designed to support the growth of insect cell lines derived from Spodoptera frugiperda.
Customized Solutions for Unique Needs
Production-Focused Approach to Produce Your Unique Media Solutions
Perfect choice for clients who have known, desired media composition and outcome expectations. For critical raw materials, you can either request specific suppliers or rely upon Sartorius' established global network of qualified suppliers. Customized packaging, media formats, and release assays are also available upon request.
Sample media for gene therapy and viral vector vaccines.
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Related Viral Vaccine Media Products
Our portfolio includes others media for Vero, MDCK, Hek 293 cells, for production of vaccines against, for example, influenza, polio, and MMR and other cell lines used in the production of viral vectors
ProVero™ 1 Serum-Free Medium Allows for High Density Vero Cultures and Good Virus Titer
ProVero™ 1 serum-free medium is protein-free and designed to support the growth of Vero and MDCK kidney cells. ProVero™ 1 Medium includes HEPES and sodium bicarbonate buffer. The absence of proteins and the very low levels of human recombinant insulin facilitate both downstream processing and regulatory compliance. Some Vero cell strains require additional supplementation with 5.0 μg/L rhEGF for optimal cell growth.
Benefit from ProMDCK™ Outperforming Serum-Free Media
ProMDCK™ are serum-free media dedicated to the growth and infection of Madin-Darby Canine Kidney (MDCK) cells in all conditions, either for research of for further manufacturing purposes. ProMDCK™ media are optimized for both expansion and virus infection of MDCK cells in planar culture conditions (2D version) and in stirred bioreactors, bound onto microcarriers (3D version).
Insect-XPRESS™ Is a Proven Easy-To-Use Medium for Viral Infection
Insect-XPRESS™ medium is a protein-free formulation designed to support the growth of insect cell lines derived from Spodoptera frugiperda (Sf9 and Sf21). High cell densities can be achieved with suspension cultures of Sf9 cells using Insect-XPRESS™ Medium and an excess of oxygen. This formulation can also be used for stationary mono-layer cultures and shake-flask cultures. Insect-XPRESS™ Medium contains L-glutamine and supports superior production of recombinant proteins with viral vectors such as Baculovirus Expression Vector System (BEVS).
Downloads
4Cell® NutriVero™ Flex 10
- 4Cell® NutriVero™ Flex 10 Medium Datasheet | English PDF | 595.2 KB
- 4Cell® NutriVero™ Flex 10 Medium Application Note | English PDF | 642.8 KB
- 4Cell® NutriVero™ Flex 10 User Manual | English PDF | 851.9 KB
4Cell® MDCK
- 4Cell ® MDCK CD Medium: Chemically Defined (CD) Medium for MDCK Cell Growth and Virus Infection in Suspension Cultures PDF | 334.8 KB
- 4Cell® MDCK CD Medium User Manual | English PDF | 478.5 KB
- 4Cell® MDCK Liquid Medium Safety Datasheet | English PDF | 214.0 KB
- 4Cell® MDCK 10L Powder Medium Safety Datasheet | English PDF | 200.1 KB
- 4Cell® MDCK 100L Powder Medium Safety Datasheet | English PDF | 200.2 KB
- 4Cell® MDCK 10L Powder Medium Safety Datasheet | Chinese PDF | 293.1 KB
- 4Cell® MDCK 100L Powder Medium Safety Datasheet | Chinese PDF | 293.1 KB
4Cell® Insect
- 4Cell® Insect CD Medium Datasheet | English PDF | 441.9 KB
- 4Cell® Insect CD Medium User Manual | English PDF | 353.4 KB
