Request Your Animal Component-Free T Cell Media
Animal Component-Free Cell Culture Media for T cells, CAR-Ts, TILs, and PBMCs
Our two chemically defined, ready-to-use media (4Cell® Nutri-T GMP Advanced and CellGenix® GMP Advanced TCM) provide excellent performance with both healthy and patient-derived donor cells. Test both media today to find the best fit for your process and cells.
In-house production of critical raw material—recombinant human albumin (Recombumin®)
Regulatory and scientific consultation and assistance
Scale-up and customization capabilities
Research Use Only, GMP available soon
4Cell® Nutri-T GMP Advanced and CellGenix® GMP Advanced TCM are offered in convenient single-use bags of different sizes, designed to support GMP-compliant manufacturing environments. These bags are available with a choice of tubing materials—thermoplastic elastomer (TPE) or polyvinyl chloride (PVC)—to accommodate different process requirements and compatibility preferences in cell and gene therapy workflows.
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Excellent Expansion and Viability with Healthy Donor-derived T Cells
4Cell® Nutri-T Advanced Medium and CellGenix® Advanced TCM support superior T cells expansion from healthy donor-derived CD3+ T cells compared to alternative chemically defined media after 10-day culture. Viability of T cells is maintained above 90%.
Long Term High Transduction Efficiency and Robust CAR Expression
4Cell® Nutri-T Advanced Medium and CellGenix® Advanced TCM support stable CAR expression and high CAR-T cell yield compared to alternative chemically defined media after 10-day culture.
Advanced T Cell Media Support Growth of Both CD4+ and CD8+ T Cells
4Cell® Nutri-T Advanced Medium and CellGenix® Advanced TCM support the growth of both CD4+ and CD8+ T cells after 10-day culture. Donor-specific variability in CD4/CD8 ratio is observed.
Stem Cell-like Memory Phenotypes were Well Maintained
Cells cultured in 4Cell® Nutri-T Advanced Medium and CellGenix® Advanced TCM display mainly undifferentiated phenotypes in both CD4+ and CD8+ T cells. In contrast, medium supplemented with serum increased the frequencies of effector memory T cells re-expressing CD45RA (Temra).
Highly Functional & Effective CD19 CAR-T Cells
CAR-T cells cultured for 7 days in our 4Cell® Nutri-T Advanced Medium and CellGenix® Advanced TCM are highly cytotoxic and specifically kill CD19-expressing NALM6 cells.
Robust Expansion and High Viability in Patient-derived CAR-T Cells
Patient-derived CAR-T cells expanded better with high viability in 4Cell® Nutri-T Advanced Medium and CellGenix® Advanced TCM.
High Transduction Efficiency with Patient-derived CAR-T Cells
Stable CAR-expression was maintained and high frequency of CD8 T cells was observed in patient-derived CAR-T cells after 10-day culture in 4Cell® Nutri-T Advanced Medium and CellGenix® Advanced TCM.
