Target ID & Validation

Target Identification and Validation

Identifying Promising Targets in Early Drug Discovery

  • Easily develop and perform high throughput target ID screens
  • Characterize a wide variety of analytes from small-molecule fragments to biologics

Identifying and characterizing promising therapeutic targets is the first step in the drug discovery process. Validating these targets becomes critical before investing additional time and resources for further development. Label-free binding technologies such as Bio-Layer Interferometry (BLI) and Surface Plasmon Resonance (SPR) systems are indispensable tools in target identification and validation processes where high throughput binding screens can be quickly established. 

Octet® systems are used to identify binding targets and accurately characterize rates of complex formation (ka, association), complex stability (kd, dissociation) and affinities (KD)

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Featured Applications

Large Molecules Kinetics Characterization

The Octet® family of instruments accurately measures kinetic constants by bringing the detection surface directly to the sample. This fluidics-free approach to label-free, real-time analysis streamlines laboratory workflows, expedites assay development and allows for direct measurement of crude samples.

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Protein - Small Molecules and Peptide Kinetics and Affinity Characterization

In small molecule drug discovery, the path to lead molecules can stem from many starting points including fragment screening or de novo structural design.  Determining and evaluating the affinity of small molecule binding to a therapeutic target is a significant component of the drug discovery process. The hit-to-lead and lead optimization processes are essential to accurately determine biological potency in vitro so structure-activity relationships (SAR) can be used for efficient structural design. The Octet® BLI platform and the Octet® SF3 SPR can be readily used to characterize small molecule and peptide lead candidates where both platforms provide a unique set of advantages from sensitivity, throughput and ready-to-use biosensors to match your workflow needs.

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Fragment Screening

Fragment-based drug design (FBDD) has become an increasingly popular platform for the identification of lead candidates in drug discovery programs. The detection and characterization of fragment binding events is facilitated by sensitive biophysical technologies capable of detecting low-affinity interactions of low molecular weight compounds. The Octet® SF3 system has the necessary sensitivity and throughput to provide complete fragment screens on libraries of several thousand compounds in just a few weeks per target.

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Featured Resources

Application Note

Advances in Immunogenicity Evaluation - Paving the Way for Safer Biolo...

Anti-drug antibodies (ADA) can modify the drug’s pharmacodynamics (PD) and/or pharmacokinetics (PK), making it crucial to detect them through an immun...

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eBook: How Are Label-Free Technologies Used in Early Drug Discovery?

How Are Label-Free Technologies Used in Early Drug Discovery?

Modern analytical techniques designed for real-time analysis and high-throughput capabilities significantly reduce the time to the discovery

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Application Note

Potency Assay Development Qualification and Validation Strategies

A method for evaluating the binding of an Fc gamma receptor III (FcγR) molecule to the widely characterized NIST mAb.

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A compendium for successful BLI and SPR assays
Application Guide

A Compendium for Successful BLI and SPR Assays

A comprehensive guide for designing and performing assays that accurately measure the binding kinetics of biomolecular interactions and analyte concen...

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Integrating Octet® BLI into Early Antibody Discovery Workflows

Improve the overall drug discovery process to increase the success rate of preclinical candidates

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Cross Competition or Epitope Binning Assays on Octet® RH96 System cover image
Application Note

Cross Competition or Epitope Binning Assays on the Octet® RH96 System

Epitope binning through cross competition assays is a powerful tool in evaluating functional diversity in antibody clones.

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Application Note

Customized Quantitation of Recombinant Therapeutic Proteins

Using Octet® SAX Biosensors

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A Rapid Method to Quantitatively Screen Bispecific Antibodies Using Protein A and Octet® His1K Biosensors
Application Note

Screen Bispecific Antibody Using Protein A and His1K Biosensors

Rapid quantitative screening of bispecific antibodies using Protein A and HIS1K biosensors

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Application Guide

Label-Free Technologies

For Accurate Determination of Kinetics and Affinity Rate Constants

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White Paper

Streamlining Affinity Analysis for Accelerated Lead Screening

Antibody and other protein therapeutics are a major focus in drug discovery pipelines today

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Cell Line Development: Accelerating Antibody Discovery by Monitoring Titer and Glycosylation With the Octet® Platform
Application Guide

Cell Line Development

Accelerating Antibody Discovery by Monitoring Titer and Glycosylation With the Octet® Platform

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Instant Access

Kinetic Analysis of Antibody Binding to an Expressed Membrane Protein on Captured Lipoparticles
Application Note

Kinetic Analysis of Antibody Binding to an Expressed Membrane Protein on Captured Lipoparticles

PDF | 929.6 KB
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Application Note

Generating Reliable Kinetic Data For Protein Ligand Interactions

PDF | 130.8 KB
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Application Note

Reducing Variability in Small Molecule Screening and Kinetics Applications

PDF | 1.4 MB

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Generate high-quality kinetics and affinity data in a fraction of the time compared to standard multi-cycle kinetics using the Octet® SF3.

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Octet® BLI rapidly determines recombinant protein concentration as a control parameter in cell line development.

96-Channel Ultra High Throughput Octet System: Octet® RH96

Monitors up to 96 biosensors simultaneously, enabling label-free detection for protein quantitation and kinetic characterization at unmatched speed

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16-Channel High Throughput Octet System: Octet® RH16

Ideal for high-throughput applications that demand high sensitivity and low sample volume requirements

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8-Channel System: Octet® R8

Unmatched flexibility and versatility in protein analysis

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