Detection of Infectious Diseases in Wastewater
Following the global outbreak of 2019, wastewater surveillance is highly recommended by the European Commission.
After thorough research and the collection of case studies, many cities and municipalities are adopting strategies to detect and monitor the genetic material of infectious diseases in wastewater. This serves as an early indicator to localize the spread and offers valuable information for tracking outbreaks and predicting new epicenters of infection.
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Based on the CDC’s National Wastewater Surveillance System (NWSS) and WastewaterSCAN Dashboard. Benefits of Wastewater Monitoring:
- Wastewater monitoring provides early detection of increasing cases.
- Wastewater monitoring is independent of medical systems.
- Wastewater monitoring is fast and efficient.
- Wastewater monitoring has national coverage.
- Wastewater monitoring can be used to track emerging health threats.
- Wastewater monitoring can be used to track changes in health threats.
- Wastewater monitoring is complementary to other public health surveillance data.
Global wastewater experts agree that further research and best practices are needed to develop and optimize appropriate sample collection, preparation, and testing approaches. Untreated wastewater samples need to be prepared easily and efficiently prior to the extraction and detection of infectious diseases.
See how we can support testing for infectious diseases in your wastewater samples.
Sartorius Solutions for Wastewater Monitoring
Concentration of Viruses and VLPs by Ultrafiltration
Ultrafiltration is an ideal and widely-used method for viral particle isolation and concentration from water and wasterwater samples prior to detection methods such as RT-PCR.
See how Vivaspin® ultrafilters (with 50 kDa MWCO PES membranes) have been used for concentration of wastewater samples prior to RT-qPCR detection of SARS-CoV-2 RNA: Post-lockdown detection of SARS-CoV-2 RNA in the wastewater of Montpellier, France
Julie Trottier, Regis Darques, Nassim Ait Mouheb, Emma Partiot, Wiliam Bakhache, Maika S Deffieu, Raphael Gaudin, medRxiv doi: https://doi.org/10.1101/2020.07.08.20148882
Membrane Filtration
A wide range of membrane filters for aqueous solutions with high flow rates. Available for particle retention in multiple diameters and pore sizes.
Particle Removal with Vacuum Filtration Units
Sartolab® RF/BT vacuum filtration units are single-use units designed for the filtration of sample volumes of up to 1 L. The filtration unit may be used as a standalone system or with the Sartolab® Multistation, which allows parallel filtration of up to six samples.
Filtration Holders & Support Accessories
Versatile, reusable all-glass vacuum filter unit and holder for analytical determinations and particle removal from solvents.
Pipetting and Sample Transfer – Picus® 2, Tacta® Mechanical Pipettes and SafetySpace Pipette Tips
As RNA preparation and RT-PCR are pipetting-intensive protocols, precise and accurate pipetting can easily be compromised. Given the importance of rapid and reliable test results, it is crucial that pipette and aerosol barrier filter tips enable quick execution while protecting samples from cross-contamination.
Ergonomically designed Sartorius mechanical Tacta® and Picus® 2 pipettes are easy to clean and resistant, making regular decontamination simple during coronavirus testing. Open connectivity enables you to integrate Picus® 2 to be part of an existing system.
RNase | DNase free pre-sterilized Safetyspace Filter tips help protect samples from contamination.
Nuclease-Free Ultrapure Water
Nuclease-free water can be utilized in several steps of RNA concentration, extraction and detection workflow, including resuspension of solid phase of wastewater samples after centrifugation and elution after binding on membranes followed by PCR detection. Sartorius’ Arium® Ultrapure Water Systems - equipped with an integrated or external ultrafilter - guarantee an on-demand supply of nuclease-free water.
