Application Note: Lab Ultrafiltration – Tips and Tricks
Although performing sample concentration and buffer exchange using an ultrafiltration device is relatively simple, some tricks of the trade can improve your recovery or speed up your workflow considerably.
This guide will give you an overview of how to:
- Desalt samples and exchange buffer in Vivaspin concentrators instead of lengthy overnight dialysis
- Concentrate samples to a defined volume in Vivaspin 500, Vivaspin Turbo 4 and Vivaspin Turbo 15
- Up to double the recovery of a very dilute sample by blocking the ultrafiltration membrane prior to concentration