Oncology Research — Exploring the hallmarks of cancer with real-time live-cell analysis
The hallmarks of cancer are continually being updated. This constantly evolving picture of tumor biology is helping to shed new light on the behavior of many cancers, as well as providing new pharmacological targets and treatment strategies. Much of this progress has been driven by technological advances, and the last decade has seen huge strides made in the field of live-cell assays, using both traditional 2D techniques and advanced 3D Cell Culture Models. Understanding the mechanisms concerned in tumor cell proliferation, death, metabolism, tumor metastasis or avoiding immune destruction may lead to limiting tumor progression and, as a result, to a reduction in mortality for many cancer patients.
The live-cell analysis capabilities of the Incucyte® Live-Cell Analysis System are revolutionizing the field of oncology research. By offering the ability to study cancer cell biology over time, in a completely non-perturbing way, this system is providing unique insights into wide range of applications.
Incucyte® Applications and Assays to Enable Oncology Research
Selective Proliferative Advantage
Study the ‘competitive advantage’ cancer cells have when it comes to cell viability or division. Leverage tumor spheroid models to derive more physiologically-relevant insight.
Invasion & Metastasis
Dissect the sequential steps of metastasis, or monitor cell function & movement in 2D or 3D cultures remodeling the extracellular matrix.
Reveal the dynamic interactions between immune and cancer cells, study immune responses or phagocytosis with wide range of non-perturbing reagents.
Characterize the aberrant metabolic activities of cancer cells providing greater insight into tumor progression, as well as potentially offering new therapeutic targets.
Sustained Proliferative Signaling
- NEW Akt Activity
Analyze dynamic changes in Akt activity for deeper insight into the effects of protein kinase activity on cell proliferation.
Resources and Products
- Kerri Wolf-Dennen, Nancy Gordon & Eugenie S. Kleinerman, 2020. Exosomal communication by metastatic osteosarcoma cells modulates alveolar macrophages to an M2 tumor-promoting phenotype and inhibits tumoricidal functions. Read more
- Mara Vinci & Chris Jones, et al., 2018. Functional diversity and cooperativity between subclonal populations of pediatric glioblastoma and diffuse intrinsic pontine glioma cells. Read more
- Salo T., et al., 2018. Organotypic three-dimensional assays based on human leiomyoma-derived matrices. Read more
- Shengguang Chen et al., 2017. PM2.5 exposure decreases viability, migration and angiogenesis in human umbilical vein endothelial cells and human microvascular endothelial cells. Read more
- Fouad, Y.A. & Aanei, C., 2017. Revisiting the hallmarks of cancer. American Journal of Cancer Research. Read more
- Hanahan, D. and Weinberg, R.A, 2011. Hallmarks of Cancer: The Next Generation. Cell. Read more