High-Speed Virus Filtration with Virosart® HF
Virosart® HF is a state-of-the-art solution for high-speed virus filtration. It is specifically designed for monoclonal antibodies (mAbs), antibody fragments (Fab), and small recombinant proteins. Its advanced polyethersulfone (PES) membrane delivers high flow rates and capacity under various processing conditions.
Virosart® HF supports robust processing by efficiently retaining both small non-enveloped viruses like MVM (LRV ≥ 4 log₁₀) and large enveloped viruses such as MuLV (LRV ≥ 6 log₁₀) from biopharmaceutical feed streams, safeguarding the integrity and quality of your product.
Challenges in Virus Clearance
Contamination of products derived from human or animal cells with viruses can have serious clinical consequences. As a result, regulatory authorities emphasize the need for robust and orthogonal virus clearance strategies. Virus filtration has long been recognized as a reliable and effective method for achieving virus clearance.
Virus Filtration Strategy for mAbs
Virosart® HF combines robust virus retention with high capacity for mAbs and recombinant proteins. It is specifically designed for easy integration into single-use processes, featuring a smart capsule design, compact footprint, and low flushing volumes.
Explore Virosart® HF
Product Overview
Performance Deep Dive
Details of Virosart® HF
Target Molecule
The main application for Virosart® HF, for virus retentive filtration, are monoclonal antibodies (mAbs), antibody fragments (Fab) or small recombinant proteins (< 150 kD).
Positioning
Virosart® HF is used towards the end of the purification process for viral filtration of the biopharmaceutical product.
Working Principle
Virus filtration works based on sizes exclusion. Small non-enveloped and large enveloped viruses are retained by the asymmetric membrane structure.
Virus Removal
- State of the art, robust virus removal performance
- ≥ 4 log10 for small non-enveloped viruses
- ≥ 6 log10 for large enveloped viruses
Cost Efficiency
- Capsule design avoids costly investments in stainless-steel filtration systems and accessories
- High filter packaging density decreases cleanroom footprint
Handling
- Includes a convenient water based integrity test to ensure reliability
- Sterile shipping options help to advance bioburden risk mitigation
Technical Proof
Retentive Capacities
The Virosart® HF delivers reliable virus removal under all circumstances with no impact from pressure variations, high loads, or process interruptions.
Performance
The Virosart® HF features high flux (340 LMH @ 2 bar) and superior capacity (>7 kg/m²), and can be optimized by a Virosart® Max pre-filter, as needed.
Knowledge Base
Download Library
Virosart® HF: High Speed Virus Filtration for mAbs and Recombinant Proteins
PDF | 3.0 MB
Training Videos
Webinars
Supporting Products & Services
Frequently Asked Questions
The Virosart® HF virus filter is used for virus filtration in biopharmaceutical applications, specifically designed to remove both small non-enveloped viruses and larger enveloped viruses from monoclonal antibodies, antibody fragments, and small recombinant proteins.
The Virosart® HF filter can effectively remove small non-enveloped viruses, such as PPV and MVM, and large enveloped viruses, such as MuLV, ensuring robust virus clearance.
The Virosart® HF filter is available in various formats, including lab, mid-scale, and process modules, with filtration areas ranging from 0.2 m² to 14.4 m², catering to different scales of development and production.
The Virosart® HF filter uses a modified polyethersulfone (PES) membrane that provides high flow rates and capacity, ensuring rapid processing and minimal hold-up volumes, which helps maintain product integrity throughout the filtration process.
Yes, the Virosart® HF filter is designed for ease of use and quick installation. It is delivered sterile, facilitating straightforward integration into biopharmaceutical processes, and is compatible with Sartocheck® technology for integrity testing.