Final Concentration and Formulation of Lentiviral Vectors Using Sartocon^® Hydrosart^® TFF Cassettes

Lentiviral vector (LV) manufacturing requires efficient downstream processing to ensure high titer, purity, and functionality. The final concentration and buffer exchange step is critical for preparing LVs for long-term storage and clinical use, and tangential flow filtration (TFF) offers a scalable solution for this purpose.  

This study provides insights into the development of a TFF unit operation using Sartocon^® Hydrosart^® 300 kDa TFF cassettes to concentrate and formulate LV feeds in a suitable buffer system, performed at the end of a typical downstream workflow in the Sartoflow^® Smart TFF benchtop system. Our findings aim to guide and contribute to the design of a robust, scalable, and efficient TFF strategy tailored for LV manufacturing.